Thidiazuron: A Potent Phytohormone for In Vitro Regeneration

  • Sujatha Govindaraj
Chapter

Abstract

Thidiazuron (C9H8N4OS) is one of the most effective substituted phenylureas that has been examined for cytokinin-like activity in plant tissue cultures. A wide range of physiological responses were ascertained in response to TDZ application in several plant species. Apart from its cytokinin-like activity, TDZ has been steered to modulate the endogenous auxin levels. However, it remains to be resolved whether it possesses an auxin activity or if it is concerned with auxin metabolism. It induces numerous morphogenic responses, starting from tissue proliferation to induction of shoot buds and somatic embryos. It has been shown to promote shoot regeneration expeditiously than that of other cytokinins, and organized centers of growth are attained at much lower concentrations. Other prospects embody modification in cell membrane, energy levels, nutrient absorption, transport, assimilation, etc. TDZ exhibits the distinctive property of mimicking both auxin and cytokinin effects on growth and differentiation of cultured explants, though structurally it is different from either auxins or purine-based cytokinins. The effectiveness of TDZ as an inductive chemical for ontogenesis is not restricted to tissue culture systems, and the regeneration is settled in vivo further. During this review, many recently revealed studies on characterization of TDZ-induced in vitro regeneration are bestowed and mentioned.

Keywords

Thidiazuron In vitro morphogenesis Callusing Somatic embryos Metabolism 

References

  1. Ahmad N, Anis M (2007) Rapid plant regeneration protocol for cluster bean (Cyamopsis tetragonoloba L. Taub.) J Hortic Sci Biotechnol 82:585–589CrossRefGoogle Scholar
  2. Ahmed MR, Anis M (2012) Role of TDZ in the quick regeneration of multiple shoots from nodal explant of Vitex trifolia L. an important medicinal plant. Appl Biochem Biotechnol 168:957–966CrossRefPubMedGoogle Scholar
  3. Alatar AA (2015) Thidiazuron induced efficient in vitro multiplication and ex vitro conservation of Rauvolfia serpentina – a potent antihypertensive drug producing plant. Biotechnol Biotechnol Equip 29(3):489–497CrossRefGoogle Scholar
  4. Arndt FR, Rusch R, Stillfried HV, Hanisch B, Martin WC (1976) A new cotton defoliant. Plant Physiol 57:S–99Google Scholar
  5. Asghar S, Abbas SJ, Wahab F, Khan NH, Ahmad N, Chen L, He X, Qin Y (2013) Direct induction of somatic embryogenesis and plant regeneration from cotyledon explants of Myrica rubra Sieb. & Zucc. Afr J Agric Res 8(2):216–223Google Scholar
  6. Babaei N, Abdullah NAP, Saleh G, Abdullah TL (2014) An efficient in vitro plantlet regeneration from shoot tip cultures of Curculigo latifolia, a medicinal plant. Sci World J 2014:1–9Google Scholar
  7. Baghel S, Bansal YK (2015) Thidiazuron promotes in vitro plant regeneration and phytochemical screening of Guizotia abyssinica Cass. – a multipurpose oil crop. World J Pharm Pharm Sci 4(1):1193–1217Google Scholar
  8. Baskakov YA, Shapovalov AA, Zhirmunskaya NM, Ovsyannikov TV (1981) Interrelationship of growth-regulating activity and phytotoxicity of synthetic cytokinins. Dokl Akad Nauk SSSR 267:1514–1517Google Scholar
  9. Burkhanova EA, Fedina AB, Baskakov YA, Yu A, Kulaeva ON (1984) Comparative study of the action of 6-benzylaminopurine, thidiazuron, and cartolin on growth of intact pumpkin seedlings. Sov Plant Physiol 31:8–12Google Scholar
  10. Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(Y2-Isopentyl) [8-14c] adenosine in callus tissues of Phaseolus lunatus L. J Plant Physiol 73(3):796–802CrossRefGoogle Scholar
  11. Caramori LPC, Favaro S, Vieira LGE (2001) Thidiazuron as a promoter of multiple shoots in cotton explants (Gossypium hirsutum L.) Acta Sci 23(5):1195–1197Google Scholar
  12. Carl AH, John EP (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119CrossRefGoogle Scholar
  13. Cassells A, Curry R (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micro-propagators and genetic engineers. Plant Cell Tissue Org Cult 64(2–3):145–157CrossRefGoogle Scholar
  14. Chandrasekhar T, Mohammad Hussain T, Rama Gopal G, Srinivasa Rao V (2006) Somatic embryogenesis of Tylophora indica (Burm.f.) Merril., an important medicinal plant. Int J Appl Sci Eng 4(1):33–40Google Scholar
  15. Davies PJ (1995) The plant hormones: their nature, occurrence, and functions. In: Davies PJ (ed) Plant hormones: physiology, biochemistry and molecular biology. Kluwer, Boston, pp 3–38CrossRefGoogle Scholar
  16. Donna IL, John EP (2004) Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr., leaf explants. Sci Hortic 101:121–126CrossRefGoogle Scholar
  17. Faisal M, Anis M (2006) Thidiazuron induced high frequency axillary shoot multiplication in Psoralea corylifolia L. Biol Plant 50:437–440CrossRefGoogle Scholar
  18. Faisal M, Shahzad A, Anis M (2008) Somatic embryogenesis and plant regeneration from nodal explants in Psoralea corylifolia L. Int J Plant Dev Biol 2(2):111–113Google Scholar
  19. Faisal M, Alatar AA, Hegazy AK, Alharbi SA, El-Sheikh M, Okla MK (2014) Thidiazuron induced in vitro multiplication of Mentha arvensis and evaluation of genetic stability by flow cytometry and molecular markers. Ind Crop Prod 62:100–106CrossRefGoogle Scholar
  20. Fathima N, Anis M (2011) Thidiazuron induced high frequency axillary shoot multiplication in Withania somnifera L. Dunal J Med Plants Res 5(30):6681–6687Google Scholar
  21. Feng JH, Chen JT (2014) A novel in vitro protocol for inducing direct somatic embryogenesis in Phalaenopsis aphrodite without taking explants. Sci World J 2014:1–7Google Scholar
  22. Gamborg OL, Phillips GC (1995) Plant cell, tissue and organ culture–fundamental methods. Springer – Verlag, BerlinCrossRefGoogle Scholar
  23. George EF (2008) Plant tissue culture procedure – background. In: George EF, Hall MA, De Kler KGJ (eds) Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht, pp 1–28Google Scholar
  24. Giridhar P, Kumar V, Indu EP, Ravishankar GA, Chandrasekar A (2004) Thidiazuron induced somatic embryogenesis in Coffea Arabica L. and Coffea canephora P ex Fr. Acta Bot Croat 63(1):25–33Google Scholar
  25. Grabkowska R, Sitarek P, Wysokińska H (2014) Influence of thidiazuron (TDZ) pretreatment of shoot tips on shoot multiplication and ex vitro acclimatization of Harpagophytum procumbens. Acta Physiol Plant 36:1661–1672CrossRefGoogle Scholar
  26. Grossmann K (1991) Induction of leaf abscission in cotton is a common effect of urea and adenine-type cytokinins. Plant Physiol 95:234–237CrossRefPubMedPubMedCentralGoogle Scholar
  27. Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: a multi-dimensional plant growth regulator. Afr J Biotechnol 10(45):8984–9000CrossRefGoogle Scholar
  28. Hamza AM, Omaima M, Kafie AE, Kasem MM (2011) Direct micropropagation of English Lavender (Lavandula angustifolia Munstead) plant. J Plant Prod 2(1):81–96Google Scholar
  29. Huettman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119CrossRefGoogle Scholar
  30. Jafari S, Daneshvar MH, Salmi MS, Abadi LJ (2017) Indirect organogenesis and plant regeneration in common sage (Salvia officinalis L.): an important medicinal plant of Iran. Mod Appl Sci 11(5):22–29CrossRefGoogle Scholar
  31. Jariteh M, Ebrahimzadeh H, Niknam V, Mirmasoumi M, Vahdati K (2015) Developmental changes in protein, proline and some antioxidant enzymes activities in somatic and zygotic embryos of Persian walnut (Juglans regia L.) Plant Cell Tissue Org Cult 122:101–115CrossRefGoogle Scholar
  32. Ji ZL, Wang SY (1988) Reduction of abscisic acid content and induction of sprouting in potato, Solanum tuberosum L., by thidiazuron. J Plant Growth Regul 7:37–44CrossRefGoogle Scholar
  33. Jones MPA, Yi ZJ, Murch SJ, Saxena PK (2007) Thidiazuron induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems. Plant Cell Rep 26:13–19CrossRefPubMedGoogle Scholar
  34. Kane ME (2005) Shoot culture procedures. In: Trigiano RN, Gray DJ (eds) Plant development and biotechnology. CRC Press, Washington, DCGoogle Scholar
  35. Kher MM, Joshi D, Nekkala S, Nataraj M, Raykundaliya DP (2014) Micropropagation of Pluchea lanceolata (Oliver & Hiern.) using nodal explant. J Hortic Res 22(1):35–39CrossRefGoogle Scholar
  36. Kokotkiewicz A, Luczkiewicz M, Hering A, Ochocka R, Gorynski K, Bucinski A, Sowinski P (2012) Micropropagation of Cyclopia genistoides, an endemic South African plant of economic importance. Zurich Nat Sci 67:65–76Google Scholar
  37. Kozai T, Xiao Y (2006) A commercialized photoautotrophic micropropagation system. In: Gupta SD, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, pp 355–371Google Scholar
  38. Lata H, Chandra S, Wang YH, Raman V, Khan IA (2013) TDZ-induced high frequency plant regeneration through direct shoot organogenesis in Stevia rebaudiana Bertoni: an important medicinal plant and a natural sweetener. Am J Plant Sci 4:117–128CrossRefGoogle Scholar
  39. Lee JM (2004) Plant cell culture and its applications. In: Goodman RM (ed) Encyclopedia of plant and crop sciences. Marcel Dekker, New York, pp 931–933CrossRefGoogle Scholar
  40. Li Z, Traore A, Maximova S, Guiltinan MJ (1998) Somatic embryogenesis and plant regeneration from floral explants of cacao (Theobroma cacao L.) using thidiazuron. In: In Vitro Cell Dev Biol Plant, vol 34, pp 293–299Google Scholar
  41. Lin CH, Lee LY, Tseng MJ (1994) The effect of stratification and thidiazuron treatment on germination and protein synthesis of Pyrus serotina Rehd. cv. Niauli. Ann Bot 73:515–523CrossRefGoogle Scholar
  42. Malabadi RB, Vijayakumar S, Nataraj K, Mulgund GS (2010) Induction of somatic embryogenesis and plant regeneration in grapes (Vitis vinifera L.) Bot Res Int 3(2):48–55Google Scholar
  43. Mok MC, Mok DWS (1985) The metabolism of [14C]-thidiazuron in callus cultures of Phaseolus lunatus. Physiol Plant 65:427–432CrossRefGoogle Scholar
  44. Moyo M, Aremu AO, Vanstaden J (2015) Insights into the multifaceted application of microscopic techniques in plant tissue culture systems. Planta 242(4):773–790CrossRefPubMedGoogle Scholar
  45. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  46. Murthy BNS, Murch SJ, andSaxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In: In Vitro Cell Dev Plant, vol 34, pp 267–275Google Scholar
  47. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87CrossRefPubMedGoogle Scholar
  48. Paul S, Dam A, Bhattacharya A, Bandyopadhyay TK (2011) An efficient regeneration system via direct and indirect somatic embryogenesis for the medicinal tree Murraya koenigii. Plant Cell Tissue Organ Cult 105:271–283CrossRefGoogle Scholar
  49. Phillips GC (2004) In vitro morphogenesis in plants – recent advances (invited review). In: In Vitro Cell Dev Biol Plant, vol 40, pp 342–345Google Scholar
  50. Pospisilova J, Ticha I, Kadlecek S et al (1999) Acclimatization of micropropagated plants in ex vitro conditions. Biol Plant 42:481–497CrossRefGoogle Scholar
  51. Prakash E, Khan SV, Meru E, Rao KR (2001) Somatic embryogenesis in Pimpinella tirupatiensis Bal. and Subr., an endangered medicinal plant of Tirumala hills. Curr Sci 81(9):1239–1242Google Scholar
  52. Reynolds AG, Wardle DA, Zurowski C, Looney NE (1992) Phenylureas CPPU and thidiazuron affect yield components, fruit composition, and storage potential of four seedless grape selections. J Am Soc Hortic Sci 1(17):85–89Google Scholar
  53. Saini R, Chopra AR (2012) In vitro plant regeneration via somatic embryogenesis in rice bean Vigna umbellata (Thunb.) Ohwi and Ohashi: an underutilized and recalcitrant grain legume. J Environ Res Dev 6(3):452–457Google Scholar
  54. Sajid ZA, Aftab F (2009) Effect of thidiazuron (TDZ) on in vitro micropropagation of Solanum tuberosum L. cvs. Desiree and Cardinal. Pak J Bot 41(4):1811–1815Google Scholar
  55. Sheibani M, Azghandi AV, Hemati SH (2007) Induction of somatic embryogenesis in Saffron using Thidiazuron (TDZ). Pak J Biol Sci 10(20):3564–3570CrossRefPubMedGoogle Scholar
  56. Siddique I, Anis M (2006) Thidiazuron induced high frequency shoot bud formation and plant regeneration from cotyledonary node explants of Capsicum annuum L. Indian J Biotechnol 5:303–308Google Scholar
  57. Sivanesan I, Hwang SJ, Jeong BR (2010) Influence of plant growth regulators on axillary shoot multiplication and iron source on growth of Scrophularia takesimensis Nakai-a rare endemic medicinal plant. Afr J Biotechnol 7:4484–4490Google Scholar
  58. Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plants tissue cultured in vitro. Symp Soc Exp Biol 11:118–131PubMedGoogle Scholar
  59. Sugawara F, Yamamoto N, Tanaka O (1994) Plant regeneration in in vitro culture of leaf, stem and petiole segments of Actinidia polygama Miq. Plant Tissue Cult Lett 11:14–18CrossRefGoogle Scholar
  60. Sujatha G, Ranjitha Kumari BD (2007) High-frequency shoot multiplication in Artemisia vulgaris L. using thidiazuron. Plant Biotechnol Rep 1:149–154CrossRefGoogle Scholar
  61. Thomas TD (2003) Thidiazuron induced multiple shoot induction and plant regeneration from cotyledonary explants of mulberry. Biol Plant 46(4):529–533CrossRefGoogle Scholar
  62. Thomas TD, Puthur JT (2004) Thidiazuron induced high frequency shoot organogenesis in callus from Kigelia pinnata L. Bot Bull Acad Sin 45:307–313Google Scholar
  63. Venglat SP, Sawhney VK (1994) Ectopic formation of trichomes and stomata in floral organs of Arabidopsis thaliana induced by thidiazuron. Can J Bot 72:671–677CrossRefGoogle Scholar
  64. Vogel G (2005) Deriving ‘controversy-free’ ES cells is controversial. Science 310:416–417CrossRefPubMedGoogle Scholar
  65. Wang SY, Steffens GL, Faust M (1986) Breaking bud dormancy in apple with a plant bioregulator, thidiazuron. Phytochemistry 25:311–317CrossRefGoogle Scholar
  66. Wang R, He LS, Xia B, Tong JF, Li N, Peng F (2009) A micropropagation system for cloning of hemp (Cannabis sativa L.) by shoot tip culture. Pak J Bot 41(2):603–608Google Scholar
  67. Zubkova NF, Bukashkina ZV, Markina LG, Belova NA (1991) Defoliating effect of fusicoccin. Agrokhimiya 12:86–92Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Sujatha Govindaraj
    • 1
  1. 1.Department of BotanyPeriyar E. V. R. College (Autonomous)TiruchirappalliIndia

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