Regulation of Morphogenesis and Improvement in Shoot Multiplication in Vitex Species Using Thidiazuron

  • Naseem Ahmad
  • Mohammad Faisal
  • Sheikh Altaf Hussain
  • Mohammad Anis


Thidiazuron (TDZ), a phenyl urea derivative, possesses strong cytokinin-like activity with a wide range of morphogenic responses in all plant species including the woody ones, where other cytokinins are least effective. The current communication reviews the effect of various concentrations and exposure durations of TDZ in in vitro morphogenesis in selected Vitex species. Four-week exposed nodal explants at 5 μM TDZ before transfer to secondary medium gave maximum shoot multiplication rate in V. negundo, while in V. trifolia 1-week exposed explants at 5.0 μM TDZ showed maximum regeneration frequency and shoot multiplication after 8 weeks of transfer to secondary medium. Best somatic embryogenesis has also been reported in leaf explants of V. doniana on TDZ-enriched medium with amino acid tryptophan. Best rhizogenesis of these TDZ-induced shootlets were reported on IBA in V. negundo and on NAA in V. trifolia. A 10-minute pulse treatment to excised shootlets at 500 μM IBA gave the maximum ex vitro rooting in V. negundo, while best in vitro rooting in V. trifolia was reported on ½ MS medium supplemented with 0.5 μM NAA. Rooted plantlets were acclimatized successfully in growth chamber and then transferred to field conditions with high survival rate without any morphological variation.


Nodal segment Thidiazuron Somatic embryogenesis In vitro rooting 



Benzyl adenine


Indole-3-butyric acid


Murashige and Skoog medium


α-Napthalene acetic acid




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Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • Naseem Ahmad
    • 1
  • Mohammad Faisal
    • 2
  • Sheikh Altaf Hussain
    • 1
  • Mohammad Anis
    • 1
  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Department of Botany & MicrobiologyCollege of Science, King Saud UniversityRiyadhSaudi Arabia

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