Detection of Cyanotoxins of Cyanobacterial (Microcystis aeruginosa) Strain Using Microtox® Bioluminescence Bioassay

  • B. Balaji Prasath
  • P. Santhanam
  • R. Nandakumar
  • T. Jayalakshmi


Cyanobacterial bloom sample was collected from Muttukadu backwaters, Chennai, Tamil Nadu, India. The bloom sample was observed under the microscope. The sample was identified as containing primarily of Microcystis aeruginosa. The occurrences of toxic cyanobacterial blooms in aquatic environments, associated with animal deaths, were noticed on 1 June 2012 and have increased the need for rapid, reliable and sensitive methods to detect algal biotoxins. Microtox® bioluminescence assay determines the toxicity of microcystin produced by cyanobacterial bloom. It is suggested that the Microtox® bioluminescence assay may prove useful in the preliminary screening of cyanobacterial blooms for microcystin-based toxicity.


Microcystis aeruginosa Microtox® assay and cyanobacterial blooms 



The authors thank the Head of the Department of Marine Science and authorities of Bharathidasan University for the facilities provided and also the DBT, Government of India, for providing financial assistance to establish the microalgae culture facility through the extramural project (BT/PR 5856/AAQ/3/598/2012). One of the authors (BB) thanks the DST, Government of India, for Fellowship, and author (RN) thanks the CSIR, Government of India, for Senior Research Fellowship.


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© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • B. Balaji Prasath
    • 2
    • 1
  • P. Santhanam
    • 2
  • R. Nandakumar
    • 2
  • T. Jayalakshmi
    • 2
    • 3
  1. 1.Beijing Normal University-Hong Kong Baptist University, United International CollegeZhuhaiChina
  2. 2.Marine Planktonology & Aquaculture Laboratory, Department of Marine Science, School of Marine SciencesBharathidasan UniversityTiruchirappalliIndia
  3. 3.National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change (MoEF&CC)ChennaiIndia

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