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Detection of Cyanotoxins of Cyanobacterial (Microcystis aeruginosa) Strain Using Microtox® Bioluminescence Bioassay

  • B. Balaji Prasath
  • P. Santhanam
  • R. Nandakumar
  • T. Jayalakshmi
Chapter

Abstract

Cyanobacterial bloom sample was collected from Muttukadu backwaters, Chennai, Tamil Nadu, India. The bloom sample was observed under the microscope. The sample was identified as containing primarily of Microcystis aeruginosa. The occurrences of toxic cyanobacterial blooms in aquatic environments, associated with animal deaths, were noticed on 1 June 2012 and have increased the need for rapid, reliable and sensitive methods to detect algal biotoxins. Microtox® bioluminescence assay determines the toxicity of microcystin produced by cyanobacterial bloom. It is suggested that the Microtox® bioluminescence assay may prove useful in the preliminary screening of cyanobacterial blooms for microcystin-based toxicity.

Keywords

Microcystis aeruginosa Microtox® assay and cyanobacterial blooms 

Notes

Acknowledgement

The authors thank the Head of the Department of Marine Science and authorities of Bharathidasan University for the facilities provided and also the DBT, Government of India, for providing financial assistance to establish the microalgae culture facility through the extramural project (BT/PR 5856/AAQ/3/598/2012). One of the authors (BB) thanks the DST, Government of India, for Fellowship, and author (RN) thanks the CSIR, Government of India, for Senior Research Fellowship.

References

  1. Agrawal, M.K., S.K. Ghosh, D. Bagchi, J. Weckesser, M. Erhard, and S.N. Bagchi. 2006. Occurrence of microcystin-containing Isolation and Characterization of Microcystin 24 toxic water blooms in Central India. Journal of Microbiology and Biotechnology 16: 212–218.Google Scholar
  2. Ahmed, M.S., S. Hillerand, and B. Lucka. 2008. Microcystis aeruginosa Bloom and the Occurrence of Microcystins (Heptapeptides Hepatotoxins) From an Aquaculture Pond in Gazipur, Bangladesh. Turkish Journal of Fisheries and Aquatic Sciences 8: 37–41.Google Scholar
  3. Balaji Prasath, B., R. Nandakumar, T. Jayalakshmi, and P. Santhanam. 2014. First report on the intense cyanobacteria Microcystis aeruginosa Kützing, 1846 bloom at Muttukkadu Backwater, Southeast coast of India. Indian Journal of Geo-Marine Sciences 43 (2): 258–262.Google Scholar
  4. Blaha, L., and B. Marsalek. 2000. Methods for detection and quantification of cyanobacterial toxins – A review. Algological Studies 99: 1–22.Google Scholar
  5. Carmichael, W.W., V. Beasley, D.L. Bunner, J.N. Eloff, I.R. Falconer, P.R. Gorham, K.I. Harada, M.J. Yu, T. Krishnamurthy, R.E. Moore, K. Rinehart, M. Runnegar, O.M. Skulberg, and M. Watnabe. 1988. Naming of cyclic heptapeptide toxins of cyanobacteria (blue-green algae). Toxicon 26: 971–973.CrossRefGoogle Scholar
  6. Frias, H.V., M.A. Mendes, K.H.M. Cardozo, V.M. Carvalho, D. Tomazela, P. Colepicolo, and E. Pinto. 2006. Use of electrospray tandem mass spectrometry for identification of microcystins during a cyanobacterial bloom event. Biochemical and Biophysical Research Communications 344: 741–746.CrossRefGoogle Scholar
  7. Kaiser, K.L.E., and J.M. Ribo. 1988. Photobacterium phosphoreum toxicity bioassay. II. Toxicity data compilation applications. Toxicity Assessment 3: 195–237.CrossRefGoogle Scholar
  8. Lappalainen, J., R. Juvonen, K. Vaajasaari, and M. Karp. 1999. A new flash method for measuring the toxicity of solid and coloured samples. Chemosphere 38: 1069–1083.CrossRefGoogle Scholar
  9. Lappalainen, J., R. Juvonen, J. Nurmi, and M. Karp. 2001. Automated color correction method for Vibrio fischeri toxicity test. Comparison of standard and kinetic assays. Chemosphere 45: 635–641.CrossRefGoogle Scholar
  10. Lawton, L.A., D.L. Campbell, K.A. Beattie, and G.A. Codd. 1990. Use of a rapid bioluminescence assay for detecting cyanobacterial microcystin toxicity. Letters in Applied Microbiology 11: 205–207.CrossRefGoogle Scholar
  11. O’Neil, J.M., T.W. Davis, M.A. Burford, and C.J. Gobler. 2012. The rise of harmful cyanobacteria blooms: The potential roles of eutrophication and climate change. Harmful Algae 14: 313–334.CrossRefGoogle Scholar
  12. Oh, H.M., S.J. Lee, J.H. Kim, H.S. Kim, and B.D. Yoon. 2001. Seasonal variation and indirect monitoring of microcystin concentrations in Daechung reservoir, Korea. Applied and Environmental Microbiology 67 (4): 1484–1489.CrossRefGoogle Scholar
  13. Oudra, B., M. Loudiki, B. Sbiyyaa, R. Martins, V. Vasconcelos, and N. Namikoshi. 2001. Isolation, characterization and quantification of microcystins (heptapeptides hepatotoxins) in Microcystis aeruginosa dominated bloom of Lalla Takerkoust lake reservoir (Morocco). Toxicon 39: 1375–1381.CrossRefGoogle Scholar
  14. Prakash, B.V., N.L. Sirisha, V.V. Satyanarayana, L. Sridevi, and B.V. Ramachandra. 2009. Aetiopathological and clinical study of erythroderma. Journal of the Indian Medical Association 107: 102–110.Google Scholar
  15. Ribo, J.M., and K.L.E. Kaiser. 1987. Photobacterium phosphoreum toxicity bioassay. I. test procedures and applications. Toxicity Assessment 2: 305–323.Google Scholar
  16. Sangolkar, L.A., S. Sarika, A. Maske, L. Pradeep, Sanjay Muthal, M. Kashyap, and Tapan Chakrabarti. 2009. Isolation and characterization of microcystin producing Microcystis from Central Indian water bloom. Harmful Algae 8: 674–684.CrossRefGoogle Scholar
  17. Sivonen, K., and G. Jones. 1999. Cyanobacterial toxins. In Toxic Cyanobacteria in Water: A Guide to Their Public Health Consequences, Monitoring and Management, ed. I. Chorus and J. Bartram, 41–111. London: E & FN Spon.Google Scholar
  18. Wang, B., and W. Li. 2002. Physiological reactions of potamogetoumalaiauus to different N and P concentrations in the growth medium. Acta Ecologica Sinica 22: 1616–1621.Google Scholar
  19. Watanabe, M.F., K. Harada, W.W. Carmichael, and H. Fujiki. 1996. Toxic microcystis, 262. New York: CRC Press.Google Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • B. Balaji Prasath
    • 2
    • 1
  • P. Santhanam
    • 2
  • R. Nandakumar
    • 2
  • T. Jayalakshmi
    • 2
    • 3
  1. 1.Beijing Normal University-Hong Kong Baptist University, United International CollegeZhuhaiChina
  2. 2.Marine Planktonology & Aquaculture Laboratory, Department of Marine Science, School of Marine SciencesBharathidasan UniversityTiruchirappalliIndia
  3. 3.National Centre for Sustainable Coastal Management, Ministry of Environment, Forest and Climate Change (MoEF&CC)ChennaiIndia

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