Analysis of Chlorophyll Fluorescence: A Reliable Technique in Determination of Stress on Plants

  • M. K. Adak


Photosynthetic research has been adopted the most informative and still remained unanswered in many aspects for plant growth and development. The basic and applied research for photosynthesis under ambient as well as natural or induced stress condition is well characterized in different plant species by the use of fluorescence technique. The description of photosynthetic process in terms of photochemistry and its quenching in heat and other forms has added much information to characterize the responses of plant genotypes. The principle, experimental setup, standardization with materials to materials, application and exercise, and finally derivation of data have been under revision for improved instrumentations. The imaging analysis of leaf under photosynthetic condition and its any deviation under stress condition has warranted the fluorescence technique as most reliable for in vivo, however, noninvasive and reliable in understanding plant’s behavior. Moreover, the amalgamation of fluorescence study through pulse amplitude modulation technique with CO2 gas exchange device has added advantages to the simultaneous recording of light-generated products in carbon reduction cycle. In reference to appropriate application of PAM measured florescence and imaging of chlorophyll fluorescence in determination, quantification and steady monitoring are customized according to instruments variation. Therefore, in this mini review, a specific focus has been set for an overview of fluorescence methodology with useful parameters, its proper use in plants’ responses, allied external factors affecting the photochemistry, the relationship with carbon reduction with acquired light energy, and further scope for up-gradation.


Chlorophyll excitation Maximum fluorescence Pulse amplitude modulated fluorescence Light harvestibg complex Photosynthetic efficiency Photon flux density Plants irradiation stress 


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© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  • M. K. Adak
    • 1
  1. 1.Plant Physiology and Plant Molecular Biology Research Laboratory, Department of BotanyUniversity of KalyaniKalyaniIndia

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