Advertisement

Super-Resolution Microscopy

  • Kazuya KabayamaEmail author
  • Ryugo Tero
Chapter

Abstract

The term “super-resolution microscopy” (SRM) includes all the optical methodologies featuring spatial resolutions exceeding the diffraction limits of optical microscopes.

Keywords

PALM STORM STED SIM Localization accuracy Stimulated emission Moiré pattern 

References

  1. 1.
    Schermelleh, L., Heintzmann, R., Leonhardt, H.: A guide to super-resolution fluorescence microscopy. J. Cell Biol. 190, 165–175 (2010)CrossRefGoogle Scholar
  2. 2.
    Yildiz, A., Forkey, J.N., McKinney, S.A., Ha, T., Goldman, Y.E., Selvin, P.R.: Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science 300, 2061–2065 (2003)CrossRefGoogle Scholar
  3. 3.
    Betzig, E., Patterson, G.H., Sougrat, R., Lindwasser, O.W., Olenych, S., Bonifacino, J.S., Davidson, M.W., Lippincott-Schwartz, J., Hess, H.F.: Imaging intracellular fluorescent proteins at nanometer resolution. Science 313, 1642–1645 (2006)CrossRefGoogle Scholar
  4. 4.
    Uno, S.N., Kamiya, M., Yoshihara, T., Sugawara, K., Okabe, K., Tarhan, M.C., Fujita, H., Funatsu, T., Okada, Y., Tobita, S., Urano, Y.: A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging. Nat. Chem. 6, 681–689 (2014)CrossRefGoogle Scholar
  5. 5.
    Hofmann, M., Eggeling, C., Jakobs, S., Hell, S.W.: Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins. Proc. Natl. Acad. Sci. U.S.A. 102, 17565–17569 (2005)CrossRefGoogle Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2018

Authors and Affiliations

  1. 1.Department of Chemistry, Graduate School of ScienceOsaka UniversityOsakaJapan
  2. 2.Department of Environmental and Life SciencesToyohashi University of TechnologyToyohashiJapan

Personalised recommendations