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Optimization of the Amplicons Detection System of Loop-Mediated Isothermal Amplification on Microfluidic Compact Disk

  • Shah Mukim Uddin
  • Fatimah Ibrahim
  • Jongman ChoEmail author
  • Kwai Lin Thong
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 64)

Abstract

Salmonellosis caused by the bacteria in the genus Salmonella remains the most important foodborne disease in both developing and developed countries. Since several decades various microbial detection and quantification methods have been developed. One of recently developed gene amplification method is loop-mediated isothermal amplification (LAMP) which has been developed on tube-based platform. To facilitate the detection of pathogenic diseases in remote areas, a microfluidic platform has been developed called lab-on-a-CD. This paper presents an improved endpoint detection system for LAMP on Lab-on-a-CD platform. A set of ultraviolet (UV) emitter and color sensor have been used in this detection system to detect the emission level of LAMP amplicons. A LAMP assay has been performed with Salmonella bacteria DNA and calibrated the duty cycle of UV emitter to optimize the amplicons detection system.

Keywords

Pathogens Salmonella Food safety Diagnosis Loop-mediated isothermal amplification LAMP Microfluidics Compact disc Lab-on-a-CD 

Notes

Acknowledgements

We would like to thank Koh Xiu Pei from Microbiology Unit, Institute of Biological Sciences, Faculty of Science, University of Malaya for the assistance to conduct LAMP assay in tube-based method.

This work is financially supported by University of Malaya (UM) research grant, UM Research Collaborative Grant Scheme (Project No: CG003-2013) and MOSTI Science Fund (Project No: GA013-2013).

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Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Shah Mukim Uddin
    • 1
    • 2
  • Fatimah Ibrahim
    • 1
    • 2
  • Jongman Cho
    • 1
    • 2
    • 4
    Email author
  • Kwai Lin Thong
    • 2
    • 3
  1. 1.Faculty of Engineering, Department of Biomedical EngineeringUniversity of MalayaKuala LumpurMalaysia
  2. 2.Faculty of EngineeringCentre for Innovation in Medical Engineering (CIME), University of MalayaKuala LumpurMalaysia
  3. 3.Microbiology Unit, Faculty of ScienceInstitute of Biological Sciences, University of MalayaKuala LumpurMalaysia
  4. 4.Department of Biomedical EngineeringInje UniversityGimhaeSouth Korea

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