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A Study of Lactic Dehydrogenase Isoenzymes in Pig

  • J. Hyldgaard-Jensen
  • S. E. Jensen
  • J. Moustgaard
  • B. Palludan
  • M. Valenta
Chapter

Summary

In the present report some results from an investigation of LDH isoenzymes in plasma, tissues and spermatozoa from pig are presented.

Three different LDH isoenzyme systems were discovered. Their characteristic behaviour is described and discussed. Polymorphism in subunit C was found. The polymorph types were found in a relatively high frequency.

Separation of LDH isoenzymes were carried out by using both agar gel and starch gel electrophoresis. In both methods certain modifications were done in order to improve sensitivity and? accuracy for the estimation of LDH isoenzymes. Investigation of LDH isoenzymes in tissues, healthy and unhealthy, indicates the possibility to obtain not only quite reliable informations about changes in progression of the disease-but also about the local metabolic conditions. The finding of two extra LDH isoenzyme systems is considered to further increase the specificity of such determinations in the clinical enzymology.

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Copyright information

© Springer Science+Business Media Dordrecht 1970

Authors and Affiliations

  • J. Hyldgaard-Jensen
    • 1
  • S. E. Jensen
    • 1
  • J. Moustgaard
    • 1
  • B. Palludan
    • 1
  • M. Valenta
    • 1
  1. 1.Department of Physiology, Endocrinology and Blood GroupingThe Royal Veterinary and Agricultural CollegeCopenhagenDenmark

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