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Pig Serum Protein Phenotyping by Electrophoresis in Thin Acrylamide Gel Slabs

  • P. Akroyd
Chapter

Summary

An improved method of acrylamide gel electrophoresis enables 20 sera to be separated in the same gel slab in 3 hr into 20–30 discrete protein zones each. The thin acrylamide gel slab is formed inside a simple glass cell mounted vertically between electrode tanks. The resulting protein zones can be specified by their migration distance relative to the albumin front — 100. The advantages and disadvantages of acrylamide are compared with starch gels.

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References

  1. Akroyd, P., 1967. Acrylamide gel slab electrophoresis in a simple glass cell for improved resolution and comparison of serum proteins, Anal. Biochem., 19, 399–410.PubMedCrossRefGoogle Scholar
  2. Akroyd, P., 1968. Chromatographic and electrophoretic techniques, vol. IL Zone electrophoresis, 458–474, 2 nd edition, Heinemann, Gt. Britain. Editor Ivor Smith.Google Scholar
  3. Ornstein, L. and Davis, B. J., 1964. Disc electrophoresis, Ann. N.Y. Acad. Sci., 121, 321–349; 404–427.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1970

Authors and Affiliations

  • P. Akroyd
    • 1
  1. 1.Colworth HouseUnilever Research LaboratoryBedfordGreat Britain

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