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Studies on Ox Red Cell Membranes: Detection of Red Cell Antigens in Ox Red Cell Protein

  • R. L. Spooner
  • A. H. Maddy
Chapter

Summary

Ox red cell membranes have been extracted with butanol. The presence of F, V, J, L, R′, S′, and T′ has been detected in the protein fraction but all activity for other blood group antigens was lost. Protein has been used for isoimmunization in cattle and produced high titre antibodies specific for the injected antigen.

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References

  1. Bednekoff, A. G., Stone, W. M., Irwin, M. R. and Link, K.P., 1958. Chemical studies on the J substance of cattle serum, Proc. Xth Int. Congr. Genet., 2.Google Scholar
  2. Hayashi, J. A., Stone, W. M., Link, K. P. and Irwin, M. R., 1958. The J substance of cattle. V. Immunochemical studies of the J substance from bovine gastric mucosa, J. Immunol., 81, 82–90.PubMedGoogle Scholar
  3. Herbert, W. J., 1965. Multiple emulsions; a new form of mineral-oil antigen adjuvant, Lancet Oct. ,16, 771.CrossRefGoogle Scholar
  4. Maddy, A. H., 1966. The properties of the protein of the plasma membrane of ox erythrocytes, Biochim. Biophys. Acta, 117, 193–200.PubMedCrossRefGoogle Scholar
  5. Watkins, W. M., 1967. “Blood-group substances” from “Specificity of cell surfaces” (Eds. Davis and Warren), Prentice-Hall, Inc., 257–279.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1970

Authors and Affiliations

  • R. L. Spooner
    • 1
    • 2
  • A. H. Maddy
    • 1
    • 2
  1. 1.Cattle Blood Typing ServiceA.R.C. Animal Breeding Research OrganizationEdinburghUK
  2. 2.Department of ZoologyUniversity of EdinburghUK

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