Immunoelectrophoretical Study on Seminal Plasma and Spermatozoa from Bulls

  • L. Veselský


By means of Immunoelectrophoresis we have observed the formation of antibodies and immunoelectrophoretic differences between spermatozoa and seminal plasma of bulls and the corresponding antibodies. We have found that bull seminal plasma forms with rabbit antiserum against seminal plasma a precipitation arc which passes through the β1 region and reaches the albumin region, further three arcs passing through the β1 region and reaching the α1 region and then one arc which is placed in the β1 region and reaches region α2 and yet another one which may be found in the β2 region. Ejaculated bull spermatozoa form with rabbit antiserum against ejaculated spermatozoa a continuous arc from the antigen reservoir up to the albumin region. This continuous arc, however, splits in the α1 and albumin regions. Another precipitation arc is formed in region β1. Ampullar spermatozoa of bulls form with rabbit antiserum against ampullar spermatozoa similar reactions. Spermatozoa from the tail of the epididymis of bulls form with rabbit antiserum against spermatozoa from the tail of the epididymis a precipitation arc which is continuous up to the α1 region only. Another arc is formed in region β1. Cross reactions between individual antigens were also investigated and their reciprocal immunological relationship was detected.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Aronsson, T. and Grønwall, A., 1957. Cited by Michalec et al., 1959. Elektroforeza na papíře a jiných nosičich, 1–135, NČSAV, Praha.Google Scholar
  2. Brummerstedt-Hansen, E., 1961. Immunoelectrophoretic investigations on the blood serum of adult pigs, Acta Vet. Scand., 2, 254–262.Google Scholar
  3. Edwards, R. G., 1960. Complement-fixing activity of normal rabbit serum with rabbit spermatozoa and seminal plasma, J. Repr. Fert., 1, 268–282.CrossRefGoogle Scholar
  4. Grabar, P., 1958. The use of immunochemical methods in studies on proteins, Advances in Protein Chemistry, 12, 1–33, Academic Press Inc.. New York.Google Scholar
  5. Henle, W., 1938. The specificity of some mammalian spermatozoa, J. Imm., 34, 325–336.Google Scholar
  6. Landsteiner, K., 1899. Cited by Weil, A. J., 1961. Antigens of the adnexal glands of the male genital tract, Fert. Ster., 12, 538–543.Google Scholar
  7. Maruta, H. and Moyer, D. L., 1967. Immunologic studies of the antigens of guinea pig semen, Fert. Ster., 18, 649–658.Google Scholar
  8. Matousek, J., 1961. Antigenic factor J in some body fluids of cattle, Folia Biol. (Praha), 7, 252–257.Google Scholar
  9. Matousek, J., 1962. The study of the antigenic characteristics of sperm from bulls, The 8th Animal Blood Group Conference in Europe, Ljubljana, Yugoslavia.Google Scholar
  10. Matousek, J., 1964. Antigenic characteristics of spermatozoa from bulls, rams and boars. III. Absorptions analyses, precipitins and fructolysis in relation to the antigenicity of bull spermatozoa, J. Repr. Fert., 8, 13–21.CrossRefGoogle Scholar
  11. Mudd S. and Mudd, E. B. H., 1929. The specificity of mammalian spermatozoa with special reference to electrophoresis as a means of serological differentiations, J. Imm., 17, 39–51.Google Scholar
  12. Pernot, E., 1956. Recherches sur les constituants antigéniques des spermatozoïdes de cobayes, Bull. Soc. Chim. Biol., 38, 1041–1054.PubMedGoogle Scholar
  13. Pernot, E. and Szumowski, P., 1958. Étude electrophorétique et immunoélectrophorétique des proteins du plasma séminal de taureau, Bull. Soc. Chim. Biol., 40, 1423–1434.PubMedGoogle Scholar
  14. Ross, V., 1946. Precipitin reactions of human seminal plasma, J. Imm., 52, 87–96.Google Scholar
  15. Scheidegger, J., 1955. Une micro-méthode de l’immunoélectrophorése, Int. Arch. Allergy Appl. Immun., 7, 103–109.CrossRefGoogle Scholar
  16. Schmid, D. O., Connealy, P. N. and Stone, W. H., 1964. Blood group antigens on bull spermatozoa, Meeting of the Midwestern Section A.S.A.S., November 27–28, Chicago, Illinois.Google Scholar
  17. Weil, A. J., Kotsevalov, O. and Wilson, L., 1956. Antigens of human seminal plasma, Proc. Soc. Exp. Biol. Med., 92, 606–610.PubMedCrossRefGoogle Scholar
  18. Weil, A. J. and Finkler, A. E., 1958. Antigens of rabbit semen, Proc. Soc. Exp. Biol. Med., 98, 794–797.PubMedCrossRefGoogle Scholar
  19. Weil, A. J., 1960. Immunological differentiation of epididymal and seminal spermatozoa of the rabbit, Science, 131, 1040–1041.PubMedCrossRefGoogle Scholar
  20. Weil, A. J. and Rodenburg, J. M., 1960. Immunological differentiations of hunam testicular (spermatocele) and seminal spermatozoa, Proc. Soc. Exp. Biol. Med., 105, 43–45.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1970

Authors and Affiliations

  • L. Veselský
    • 1
  1. 1.Laboratory of Physiology and GeneticsCzechoslovak Academy of SciencesLiběchovCzechoslovakia

Personalised recommendations