Physicochemical and Immunological Basis of Immunoglobulin Heterogeneity

  • B. Zabłocki


The history of immunoglobulins is still relatively short, their definition was introduced only 31 years ago by Tiselius (1937), Tiselius and Kabat (1939). Peculiar characteristics of these proteins are: (a) antibody activity (b) they are the slowest migrating electrophoretic group of serum proteins, (c) extensive heterogeneity, (d) they are formed in plasmacytes and lymphoid cells.


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  1. Allen, P. Z., Shisinha S. and Vaughan, J. H., 1965. J. Immunol., 95, 918.PubMedGoogle Scholar
  2. Anders, G. A., Hsu, K. C. and Seegal, B.C., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 527–570.Google Scholar
  3. Backhausz, R., Veres, G. and Veto, I., 1960. Ann. Immunol. Hungariae., 3, 116.Google Scholar
  4. Baglioni, C. and Cioli, D., 1966. J. Exp. Med., 124, 307.PubMedCentralPubMedGoogle Scholar
  5. Baglioni, C., Zonta, L. A., Cioli, D. and Carbonara, A., 1966. Science, 152, 1517.PubMedGoogle Scholar
  6. Baker, A. R., Bloch, K. J. and Austen, K. F., 1964. J. Immunol., 93, 525.PubMedGoogle Scholar
  7. Bassett, E. W., Tanenbaum, S. W., Pryzwansky, K., Beiser, S. M. and Kabat, E. A., 1965. J. Exp. Med., 122, 251.PubMedCentralPubMedGoogle Scholar
  8. Benacerraf, B., Ovary, Z., Bloch, K. J. and Franklin, E. C., 1963. J. Exp. Med., 117, 937.PubMedCentralPubMedGoogle Scholar
  9. Berken, A., and Benacerraf, B., 1966. J. Exp. Med., 123, 119.PubMedCentralPubMedGoogle Scholar
  10. Bernier, G. M. and Putnam, F. W., 1963. Nature, 200, 223.PubMedGoogle Scholar
  11. Berson, S. A., Yalow, R. S., Baumant, A., Rothschild, M. A. and Newerby, K., 1956. J. Clin. Invest., 35, 170.PubMedCentralPubMedGoogle Scholar
  12. Boyden, S. V., 1951. J. Exp. Med., 93, 107.PubMedCentralPubMedGoogle Scholar
  13. Buchowicz, I., 1966. Postçpy Biochemii, 12, 5.Google Scholar
  14. Campbell, R. M., Cuthbetson, D. P., Matthews, C. M. E., McFarlane, A. S., 1956. Int. J. Appl. Radiation and Isotopes, 1, 66.Google Scholar
  15. Charlwood, P. A., 1966. Brit. Med. Bull., 22, 121.PubMedGoogle Scholar
  16. Coe, J. E., 1966. Immunochemistry, 3, 427.Google Scholar
  17. Cohen, S., 1963. Biochem. J., 89, 334.PubMedGoogle Scholar
  18. Cohen, S., 1965. Biochem. J., 95, 136.Google Scholar
  19. Cohen, S., Chaplin, H. and Press, E. M., 1965. Biochem. J., 95, 259.Google Scholar
  20. Cohen, S. and Milstein, C., 1967. Nature, 214, 449.PubMedGoogle Scholar
  21. Cohen, S. and Porter, R. R., 1964. Adv. Immunol., 4, 287.Google Scholar
  22. Cohn, E. J. et al, 1946. J. Am. Chem. Soc, 68, 459.PubMedGoogle Scholar
  23. Cohn, E. J. et al, 1950. J. Am. Chem. Soc, 72, 465.Google Scholar
  24. Connell, G. E. and Painter, R. H., 1966. Canad. J. Biochem., 44, 371.Google Scholar
  25. Coons, A. H., Creech, H. J. and Jones, R. N., 1941. Broc. Soc. Exp. Biol. (N. Y.), 47, 200.Google Scholar
  26. Coons, A. H. and Kaplan, M. H., 1950. J. Exp. Med., 91, 1.PubMedCentralPubMedGoogle Scholar
  27. Crumpton, M. J. and Wilkinson, J. M., 1963. Biochem. J., 88, 228.PubMedGoogle Scholar
  28. Edelman, G. M. and Benacerraf B., 1962. Proc. Nat. Acad. Sci. (Wash.), 48, 1035.PubMedGoogle Scholar
  29. Edelman G. M., Benacerraf, B. and Ovary, Z., 1963. J. Exp. Med., 118, 229.PubMedCentralPubMedGoogle Scholar
  30. Edelman, G. M. and Poulik, M. D., 1961. J. Exp. Med., 113, 861.PubMedCentralPubMedGoogle Scholar
  31. Edman, P. and Sjoquist, J., 1956. Acta Chem. Scand., 10, 1507.Google Scholar
  32. Eisen, H. N., 1964a. Methods in Medical Research, 10, 115.PubMedGoogle Scholar
  33. Eisen, H. N., 1964b. Methods in Medical Research, 10, 94.PubMedGoogle Scholar
  34. Eisen, H. N., 1964c. Methods in Medical Research, 10, 106.PubMedGoogle Scholar
  35. Eisen, H. N. and Karush, F., 1949. J. Am. Chem. Soc., 71, 363.PubMedGoogle Scholar
  36. Elek, S. D., 1948. Brit. Med. J., 1, 483.Google Scholar
  37. Elliott, M., Englberger, F. M. and Gaddone, S. M., 1962. J. Immunol., 89, 517.Google Scholar
  38. Fahey, J. L., 1961a. J. Exp. Med., 114, 385.PubMedCentralPubMedGoogle Scholar
  39. Fahey, J. L., 1961b. J. Exp. Med., 114, 389.Google Scholar
  40. Fahey, J. L., 1962. Adv. Immunol., 2, 42.Google Scholar
  41. Fahey J. L., Wunderlich, J. and Mishell, R., 1964. J. Exp. Med., 120, 223.PubMedCentralPubMedGoogle Scholar
  42. Farah, F. S., Kern, M. and Eisen, H. N., 1960. J. Exp. Med., 112, 1195.PubMedCentralPubMedGoogle Scholar
  43. Feinstein, A., Gell, P. G. H. and Kelus, A. S., 1963. Nature, 200, 653.PubMedGoogle Scholar
  44. Fleischman, J.B., Pain, R. and Porter, R. R., 1961. Arch. Biochem. Biophys., Suppl., 1, 174Google Scholar
  45. Fleischman, J. B., Porter, R. R. and Press, E. M., 1963. Biochem. J., 88, 220.PubMedGoogle Scholar
  46. Fongereau, M. and Edelman, G. M., 1965. J. Exp. Med., 121, 373.Google Scholar
  47. Franek, F., 1961. Biochem. Biophys. Res. Comm., 4, 28.PubMedGoogle Scholar
  48. Franek, F. and Nezlin, R. S., 1963. Folia Microbiol. (Prague), 8, 128.Google Scholar
  49. Frangione, B., Franklin, E. C., Fudenberg, H.H. and Koshland, M. E., 1966. J. Exp. Med., 124, 715.PubMedCentralPubMedGoogle Scholar
  50. Franklin, E. C., 1960. J. Clin. Invest., 39, 1933.PubMedCentralPubMedGoogle Scholar
  51. Franklin, E. C., 1964. Progress in Allergy, 8, 58.Google Scholar
  52. Franklin, E. C., Fudenberg, H. H., Meltzer, M. and Stanwort, D. R., 1962. Proc.Nat. Acad. Sci. (Wash.), 48, 904.Google Scholar
  53. Freeman, T., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 597–607.Google Scholar
  54. Fudenberg, H. H., 1965. Ann. Rev. Microbiol, 19, 301.Google Scholar
  55. Fudenberg, H. H., Feinstein, D., McGehee, W. and Franklin, E. C., 1966. Vox Sang., 11, 45.PubMedGoogle Scholar
  56. Gitlin, D. and Merler, E., 1961. J. Exp. Med., 114, 207.Google Scholar
  57. Grabar, P. and Burtin, P., 1964. Immunoelectrophoretic analysis, Elsevier, Amsterdam.Google Scholar
  58. Grabar, P. and Williams, C. A., 1953. Biochim. Biophys. Acta, 10, 193.PubMedGoogle Scholar
  59. Gray, W. R. and Hartley, B. S., 1963. Biochem. J., 89, 59P.Google Scholar
  60. Grey, H. M. and Kunkel, H. G., 1964. J. Exp. Med., 120, 252.Google Scholar
  61. Grossberg, A. and Pressman, D., 1964. Methods in Medical Research, 10, 103.PubMedGoogle Scholar
  62. Haber, E. and Bennett, J. C., 1962. Proc. Nat. Acad. Sci., 48, 1935.PubMedGoogle Scholar
  63. Hanson, L. A. and Johansson, B. G., 1963. Clin. Chim. Acta, 8, 66.PubMedGoogle Scholar
  64. Harboe, M. and Deverill, J., 1964. Scand. J. Haemat., 1, 223.PubMedGoogle Scholar
  65. Heimberger, N., Heide, K., Haupt, H. and Schultze, H. E., 1964. Clin. Chim. Acta, 10, 293.Google Scholar
  66. Herbert, W. J., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 720–744.Google Scholar
  67. Heremans, J. F., 1960. Les Globulines sériques du système gamma, Ar scia, Brussels.Google Scholar
  68. Hill, W. C. and Cebra, J. J., 1964. Biochemistry, 4, 2575.Google Scholar
  69. Hilschman, N. and Craig, L.C., 1965. Proc. Nat. Acad. Sci. (Wash, ), 153, 1403.Google Scholar
  70. Hsiao, S. and Putnam, F. W., 1961. J. Biol. Chem., 236, 122.PubMedGoogle Scholar
  71. Hunter, W. M., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 608–654.Google Scholar
  72. Ingraham, J. S., 1958. Proc. Soc. Exp. Biol. (N.Y.), 99, 452.PubMedGoogle Scholar
  73. Ingraham, J. S. and Bussard, A., 1964. J. Exp. Med., 119, 667.PubMedCentralPubMedGoogle Scholar
  74. Irrverre, F., 1965. Biochim. Biophys. Acta, 111, 551.Google Scholar
  75. Ishizaka, K., Ishizaka, T. and Hathorn E. M., 1964. Immunochemistry, 1, 197.PubMedGoogle Scholar
  76. Ishizaka, K., Ishizaka, T. and Hornbrook, M. M., 1966. J. Immunol., 97, 75.PubMedGoogle Scholar
  77. James, K., Henney, C. S. and Stanworth, D. R., 1963. Nature, 202, 563.Google Scholar
  78. Jerne, N. K. and Nordin, A. A., 1963. Science, 140, 405.Google Scholar
  79. Johson, A., Day, E. D. and Pressman, D., 1960. J. Immunol., 84, 213.Google Scholar
  80. Kabat, E.A. and Mayer, M. M., 1962. Experimental immunochemistry, Thomas, Springfield.Google Scholar
  81. Koshland, M. E., Englberger, F. M. and Shapanka, R., 1964. Science, 143, 1330.Google Scholar
  82. Koshland, M. E. and Englberger, F. M., 1963. Proc. Nat. Acad. Sel, 50, 61.Google Scholar
  83. Koshland, M. E., Englberger, F. M. and Gaddone, S. M., 1963. J. Biol. Chem., 238, 1349.PubMedGoogle Scholar
  84. Koshland, M. E., Englberger, F. M. and Shapanka, R., 1966. Biochemistry, 5, 641.PubMedGoogle Scholar
  85. Lamm, M. E., Nussezweig, V. and Benacerraf, B., 1966. Immunology, 10, 309.PubMedGoogle Scholar
  86. Laurell, C. B., 1965. Analyt. Biochem., 10, 358.PubMedGoogle Scholar
  87. Lawler, S. D. and Cohen, S., 1965. Immunology, 8, 206.PubMedGoogle Scholar
  88. Lichter, E. A., 1964. Proc. Soc. Exp. Biol Med., 113, 555.Google Scholar
  89. Longsworth, L. G., 1959. Electrophoresis, Ed. M. Bier, Academic Press, New York, pp. 91, 137.Google Scholar
  90. Mannik, M. and Kunkel, H. G., 1963. J. Exp. Med., 117, 213.PubMedCentralPubMedGoogle Scholar
  91. Margoliasch, P., 1962. J. Biol. Chem., 237, 2161.Google Scholar
  92. Meltzer, M., Franklin, E. C., Fudenberg, H. H. and Frangione, B., 1964. Proc. Nat. Acad. Sci. (Wash.), 51, 1007.PubMedGoogle Scholar
  93. Metzger, H. and Singer, S. J., 1963. Science, 142, 674.PubMedGoogle Scholar
  94. Metzger, H., Wofsy, L. and Singer, S. J., 1963. Biochemistry, 2, 979.PubMedGoogle Scholar
  95. Middlebrook, G. and Dubos, K. J., 1948. J. Exp. Med., 88, 521.PubMedCentralPubMedGoogle Scholar
  96. Müller-Eberhard, H. J., 1960. Scand. J. Clin. Labor. Invest., 12, 33Google Scholar
  97. Nairn, R. C., 1964. Fluorescent protein tracing, Livingstone, Edinburgh.Google Scholar
  98. Nezlin, R. S., 1966. Biokhimia ajititiel, Izd. “Nauka”, Moscow, 59–178.Google Scholar
  99. Nissonoff, A., Wissler, F. C. and Lipman, L. N., 1960. Science, 132, 1770.Google Scholar
  100. Nissonoff, A., Wissler, F. C., Lipman, L. N. and Woernley, D. L., 1960. Arch. Biochem. Biophys., 89, 230.Google Scholar
  101. Nissonoff, A., Wissler, F. C. and Woernley, D. L., 1959. Biochem. Biophys. Res. Comm., 1, 318.Google Scholar
  102. Oakley, C. L. and Fulthrope, A. J., 1953. J. Path. Bact., 65, 49.PubMedGoogle Scholar
  103. Olins, D. E. and Edelman, G. M., 1962. J. Exp. Med., 116, 635.PubMedCentralPubMedGoogle Scholar
  104. Onoue, K., Yagi, Y. and Pressman, D., 1966. J. Exp. Med., 123, 173.PubMedCentralPubMedGoogle Scholar
  105. Ouchterlony, O., 1948. Acta Path. Microbiol. Scand., 52, 186.Google Scholar
  106. Ouchterlony, O., 1958. Progressin Allergy, 5, 1.Google Scholar
  107. Ouchterlony, O., 1962. Progress in Allergy, 6, 30.PubMedGoogle Scholar
  108. Oudin, J., 1946. C. R. Acad. Sci., 222, 115.Google Scholar
  109. Oudin, J., 1952. Methods in Medical Research, 5, 335.PubMedGoogle Scholar
  110. Ovary, Z., 1952. Int. Arch. Allergy Appl. Immunol., 3, 293.PubMedGoogle Scholar
  111. Ovary, Z., 1960. Immunology, 3, 19.PubMedGoogle Scholar
  112. Ovary, Z., 1964. Immunological Methods, Ed. Ackroyd J. F., Blackwell, Oxford, 259–283.Google Scholar
  113. Ovary, Z., Bloch, K. J. and Benacerraf, B., 1964. Proc. Soc. Exp. Biol. Med., 116, 840.PubMedGoogle Scholar
  114. Parker, C. W., 1963. Conceptual advances in immunology and oncology, Harper, New York, 191–205.Google Scholar
  115. Parker, C. W., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 423–462.Google Scholar
  116. Patterson, R., Pruzansky, J. J. and Janis, B., 1964. J. Immunol., 93, 51.PubMedGoogle Scholar
  117. Patterson, R. and Spark, D. B., 1962. J. Immunol., 88, 262.PubMedGoogle Scholar
  118. Pennell, R. B., 1960. The plasma proteins, Ed. Putnam F.W., Academic Press, New York, vol. 1, p. 9.Google Scholar
  119. Peterson, E. A. and Sober, H. A., 1956. J. Am. Chem. Soc, 78, 751.Google Scholar
  120. Peterson, E. A. and Sober, H.A., 1960. The plasma protein, Ed. Putnam F. W., Academic Press, New York.Google Scholar
  121. Pinckard, R. N. and Weir, D. M., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 493–526.Google Scholar
  122. Porter, R. R., 1959, Biochem. J., 73, 119.PubMedGoogle Scholar
  123. Press, E. M., 1965. Symposium on Molecular and Cellular Basis of Antibody Formation, Prague, p. 93.Google Scholar
  124. Press, E. M., Piggot, P. J. and Porter, R. R., 1966. Biochem. J., 99, 356.PubMedGoogle Scholar
  125. Press, E. M. and Porter, R. R., 1966. Immunoglobulins. Glycoproteins, Ed. Gottschalk A. and Neuberger A., Elsevier, Amsterdam.Google Scholar
  126. Pressman, D., Campbell, D. H. and Pauling, L., 1942. J. Immunol., 44, 101.Google Scholar
  127. Pressman, D. and Roholt, O. A., 1961. Proc. Nat. Acad. Sci. (Wash.), 47, 160C.Google Scholar
  128. Putnam, F. W., 1965. The proteins, Ed. Neurath H., vol. Ill, Academic Press, New York, 153–267.Google Scholar
  129. Putnam, F. W. and Easley, C. W., 1965. J. Biol. Chem., 240, 1626.PubMedGoogle Scholar
  130. Putnam, F. W., Easley, C. W. and Lynn, L. T., 1962. Biochem. Biophys. Acta, 58, 279.PubMedGoogle Scholar
  131. Raymond, S., 1962. Clin. Chem., 8, 455.PubMedGoogle Scholar
  132. Rockey, J. H., Klinman, N. R. and Karush, F., 1964. J. Exp. Med., 120, 589.PubMedCentralPubMedGoogle Scholar
  133. Rosevar, J. W. and Smith, E. L., 1958. J. Am. Chem. Soc, 80, 250.Google Scholar
  134. Rosevar, J. W. and Smith, E. L., 1961. J. Biol. Chem., 236, 425.Google Scholar
  135. Sanger, F., 1945. Biochem. J., 39, 507.PubMedGoogle Scholar
  136. Scheidegger, J. J., 1955. Int. Arch. Allergy, 7, 103.PubMedGoogle Scholar
  137. Schultze, H. E., Haupt, H., Heide, K., Heimberger, N. and Schwick, H. G., 1965. Immuno-chemistry, 2, 273.Google Scholar
  138. Seijen, H. G. and Gruber, M., 1963. J. Molec. Biol, 7, 209.PubMedGoogle Scholar
  139. Sela, M. and Mozes, E., 1966. Proc. Nat. Acad. Sci. (Wash.), 55, 445.PubMedGoogle Scholar
  140. Sell, S., 1967. Immunochemistry, 4, 49.PubMedGoogle Scholar
  141. Singer, S. J., 1959. Nature, 183, 1523.PubMedGoogle Scholar
  142. Skalba, D., 1967. Postepy Mikrobiologii, 6, 403.Google Scholar
  143. Skeggs, L. T., Jr. and Hochstrasser, H., 1962. Ann. N.Y. Acad. Sci., 102, 144.PubMedGoogle Scholar
  144. Skvaril, F., 1960. Nature, 185, 475.PubMedGoogle Scholar
  145. Smith, E. L. and Jager, B. V., 1952. Ann. Rev. Microbiol, 6, 207.Google Scholar
  146. Smithies, O., 1959. Adv. Protein Chem., 14, 65.PubMedGoogle Scholar
  147. Sober, H., Hartley, R., Carrol, W. and Peterson, E., 1965. The proteins, Ed. Neurath H., vol. III, Academic Press, New York, 1–97.Google Scholar
  148. Stanworth, D. R., 1967. Handbook of experimental immunology, Ed. Weir D.M., Blackwell, Oxford, 44–111.Google Scholar
  149. Stanworth, D. R. and Pardoe, G.I., 1967. Handbook of experimental immunology, Ed. Weir D. M., Blackwell, Oxford, 134–328.Google Scholar
  150. Stavitsky, A.B., 1964. Immunological methods, Ed. Acroyd J. F., Blackwell, Oxford, 363–396.Google Scholar
  151. Svenson, H., 1960. A laboratory manual of analytical methods in protein chemistry, Eds. Alexander P., Bloc R. J., Macmillan, New York, 193.Google Scholar
  152. Terry, W. D. and Fahey, J. L., 1964. Science, 146, 400.PubMedGoogle Scholar
  153. Thorbecke, G. J., Benacerraf, B. and Ovary, Z., 1963. J. Immunol., 91, 670.PubMedGoogle Scholar
  154. Tiselius, A., 1937. Biochem. J., 31, 1464.PubMedGoogle Scholar
  155. Tiselius, A. and Kabat, E. A., 1939. J. Exp. Med., 69, 119.PubMedCentralPubMedGoogle Scholar
  156. Titani, K., Whitley, E., Avogadro, L. and Putnam, F. W., 1965. Science, 149, 1090.PubMedGoogle Scholar
  157. Trautman, R., 1964. Instrumental methods of experimental biology, Ed. Neuman D. N., MacMillan, New York.Google Scholar
  158. Utsumi, S. and Karush, F., 1963. Fed. Proc, 22, 466.Google Scholar
  159. Utsumi, S. and Karush, F., 1965. Biochemistry, 4, 1766.Google Scholar
  160. Vaerman, J. P., Epstein, W., Fudenberg, H. H. and Ishizaka, K., 1964. Nature, 203, 1046.PubMedGoogle Scholar
  161. Weir, D. M. (Ed.), 1967. Handbook of experimental immunology, Blackwell, Oxford, pp. 1245.Google Scholar
  162. Williams, J. W., 1963. Ultracentrifugation in theory and experiments, Academic Press, New York.Google Scholar
  163. Winsten, S., Friedman, H. and Schwartz, E. E., 1963. Analyt. Biochem., 6, 404.PubMedGoogle Scholar
  164. Wofsy, L., Metzger, H. and Singer, S. J., 1962. Biochemistry, 1, 1031.PubMedGoogle Scholar
  165. Wofsy, L. and Singer, S. J., 1963. Biochemistry, 2, 104.PubMedGoogle Scholar
  166. Wunderlich, J., Mishell, R. and Fahey, J. L., 1964. J. Exp. Med., 120, 243.PubMedCentralPubMedGoogle Scholar
  167. Wunderly, C., 1960. J. Chromatog., 3, 536.Google Scholar
  168. Zablocki, B., 1965. Postepy Mikrobiologii, 4, 135.Google Scholar
  169. Zablocki, B., 1966. Postepy Mikrobiologii, 5, 17.Google Scholar

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© Springer Science+Business Media Dordrecht 1970

Authors and Affiliations

  • B. Zabłocki
    • 1
  1. 1.Department of MicrobiologyUniversity of ŁódźŁódźPoland

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