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An Oral Vaccine in Maize Protects Against Transmissible Gastroenteritis Virus in Swine

  • J. Jilka
Chapter

Abstract

Transmissible gastroenteritis (TGE) is a highly contagious enteric disease that is characterized by vomiting, severe diarrhea and high mortality in piglets less than two weeks of age. The development of edible vaccines offers the potential to aid in the control of enteric diseases such as TGE. Edible vaccines from plant material could be directly delivered in the feed and could be produced cheaply in large volumes thus avoiding many costs associated with the administration of conventional vaccines. Vaccines from plants are particularly suitable for stimulation of mucosal immunity, since edible plant products can be delivered orally to reach the gut mucosal tissue and elicit an immune response at mucosal surfaces. Recent advances in technology make it now possible to express vaccine antigens at high levels in plants. Corn expressing the S protein of TGEV was fed to 13-day-old piglets for ten days and subsequently challenged with a virulent Purdue strain of TGEV. This group of piglets was significantly protected from the disease as contrasted to the control group that was fed only corn. Results from a second trial duplicated these results demonstrating that the delivery of antigens delivered in an edible oral form are efficacious

Keywords

antigen mucosal immunity transgenic plants TGEV enteric pathogen 

References

  1. Ahmad S, Lohman B, Marthas M, Giavedoni L, Amad Z, Haigwood NL, Scandella CJ, Gardner MB, Luciw PA and Yilma T, 1994. Reduced virus load in rhesus macaques immunized with recombinant gp160 and challenged with simian immunodeficiency virus. AIDS Res. Hum. Retroviruses, 10: 195–204PubMedCrossRefGoogle Scholar
  2. Arakawa T, Chong DK, Merritt JL and Langridge WH, 1997. Expression of cholera toxin B subunit oligomers in transgenic potato plants. Transgenic Res, 6: 403–413PubMedCrossRefGoogle Scholar
  3. Arakawa T, Yu J and Langridge WH, 1999. Food plant-delivered cholera toxin B subunit for vaccination and immunotolerization. Adv Exp Med Biol, 464: 161–178PubMedCrossRefGoogle Scholar
  4. Bergmeier LA, Mitchell EA, Hall G, Cranage MP, Cook N, Dennis M and Lehner T, 1998. Antibody-secreting cells specific for simian immunodeficiency virus antigens in lymphoid and mucosal tissues of immunized macaques. AIDS, 12: 1139–1147PubMedCrossRefGoogle Scholar
  5. Czerkinsky C, Svennerholm AM and Holmgren J, 1993. Induction and assessment of immunity at enteromucosal surfaces in humans: implications for vaccine development. Clin Infect Dis 16 Suppl, 2: 5106–116Google Scholar
  6. Garvey PB, Hammond J, Dienelt MM, Hooper DC, Fu ZF, Dietzschold B, Koprowski H and Michaels FH, 1995. Expression of the rabies virus glycoprotein in transgenic tomatoes. Biotechnology, 13: 1484–1487CrossRefGoogle Scholar
  7. Gomez N, Carrillo C, Salinas J, Parra F, Borca MV and Escribano JM, 1998. Expression of immunogenic glycoprotein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants. Virology, 249: 352–358PubMedCrossRefGoogle Scholar
  8. Guidry JJ, Cardenas L, Cheng E and Clements JD, 1997. Role of receptor binding in toxicity, immunogenicity, and adjuvanticity of Eschericia coli heat-labile enterotoxin. Immunology 65: 4943–4950Google Scholar
  9. Hag TA, Mason HS, Clements J and Arntzen CJ, 1995. Production of an orally immunogenic bacterial protein in transgenic plants: proof of concept of edible vaccines. Science, 268: 714–716CrossRefGoogle Scholar
  10. Holmgren J, Czerkinsky C, Lycke N and Svennerholm AM, 1994. Strategies for the induction of immune responses at mucosal surfaces making use of cholera toxin B subunit as immunogen, carrier, and adjuvant. Am J Trop Med Hyg, 50: 42–54PubMedGoogle Scholar
  11. Hood EE, Withcher DR, Maddock S, Meyer T. Baszczynski CBM, Flynn P, Register J, Marshal L, Bond D, Kulisek E, Kusnadi A, Evangelista R, Nikolov Z, Wooge C, Mehigh RJ, Hernan R, Kappel WK, Ritland D, Li PC and Howard JA, 1997. Commercial production of avidin from transgenic maize: characterization of transformant, production, processing, extraction and purification. Mol Breeding, 3: 291–306CrossRefGoogle Scholar
  12. Ishida Y, Saito H, Ohta S, Hiei Y, Komari T and Kumashiro T, 1996. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol, 14: 745–750PubMedCrossRefGoogle Scholar
  13. Kapusta J, Modelska A, Figlerowicz M, Pniewski T, Letellier M, Lisowa O, Yusibov V, Koprowski H, Plucienniczak A and Legocki AB, 1999. A plant-derived edible vaccine against hepatitis B virus. FASEB J, 13: 1796–1799PubMedGoogle Scholar
  14. Kusnadi AR, 1998. Production and purification of two recombinant proteins from transgenic corn. Biotechnol Prog, 14: 149–155PubMedCrossRefGoogle Scholar
  15. Kusnadi A, Evangelista R, Hood EE, Howard JA and Nikolov Z, 1998. Processing of transgenic corn seed and its effect on the recovery of recombinant beta-glucuronidase. Biotechnol Bioeng, 60: 44–52PubMedCrossRefGoogle Scholar
  16. Lu X, Kiyono H, Lu D, Kawabata S, Torten J, Srinivasan S, Dailey PJ, McGhee JR, Lehner T and Miller CJ, 1998. Targeted lymph-node immunization with whole inactivated simian immunodeficiency virus (SIV) or envelope and core subunit antigen vaccines does not reliably protect rhesus macaques from vaginal challenge with SIVmac251. AIDS, 12: 1–10PubMedCrossRefGoogle Scholar
  17. Mason HS, Ball JM, Shi JJ, Jiang X, Estes MK and Arntzen CJ, 1996. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci USA, 93: 5335–5340PubMedCrossRefGoogle Scholar
  18. Mason HS, Haq TA, Clements JD and Arntzen CJ, 1998. Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene. Vaccine, 16: 1336–1343PubMedCrossRefGoogle Scholar
  19. Modelska A, Dietzschold B, Sleysh N, Fu ZF, Steplewski K, Hooper DC, Koprowski H and Yusibov V, 1998. Immunization against rabies with plant-derived antigen. Proc Nati Acad Sci USA, 95: 2481–2485CrossRefGoogle Scholar
  20. Moldoveanu Z, Vzorov AN, Huang WQ, Mestecky J and Compans RW, 1999. Induction of immune responses to SIV antigens by mucosally administered vaccines [In Process Citation]. AIDS Res Hum Retroviruses, 15: 1469–1476PubMedCrossRefGoogle Scholar
  21. Pen J, Molendijk L, Quax WJ, Sijmons PC, Ooyen AJ van, Elzen PJ van den, Rietveld K, and Hoekema A, 1992. Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction. Bio/Technology, 10: 292–296PubMedCrossRefGoogle Scholar
  22. Rogers IC, 1985. Two barley alpha-amylase gene families are regulated differently in aleurone cells. J Biol Chem, 260: 3731–3738PubMedGoogle Scholar
  23. Ruedl C and Wolf H, 1995. Features of oral immunization. Int Arch Allergy Immunol, 108: 334–339PubMedCrossRefGoogle Scholar
  24. Saif LJ and Wesley RD, 1992. Transmissible gastroenteritis. In Diseases of swine, 7`h Edition, Iowa State University Press 29, pp 362–386Google Scholar
  25. Saif LJ, Cott JL van and Brim TA, 1994. Immunity to transmissible gastroenteritis virus and porcine respiratory coronavirus infections in swine. Vet Immunol Immunopathol, 43: 89–97PubMedCrossRefGoogle Scholar
  26. Thanavala Y, Yang Y-F, Lyons P, Mason HS and Arntzen CJ, 1995. Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc Natl Acad Sci USA, 92: 3358–3361PubMedCrossRefGoogle Scholar
  27. Trudel J, Potvin C and Asselin A, 1992. Expression of active hen egg white lysozyme in transgenic tobacco. Plant Sci, 87: 55–67CrossRefGoogle Scholar
  28. Tuboly T, Yu W, Bailey A, Degrandis S, Erickson L, Du S and Nagy E, 2000. Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants. Vaccine, 18: 2023–2028PubMedCrossRefGoogle Scholar
  29. Wigdorovitz A, Carrillo C, Dus Santos MJ, Trono K, Peralta A, Gomez MC, Rios RD, Franzone PM, Sadir AM, Escribano IM and Borca MV, 1999. Induction of a protective antibody response to foot and mouth disease virus in mice following oral or parenteral immunization with alfalfa transgenic plants expressing the viral structural protein VP 1. Virology, 255: 347–353Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2002

Authors and Affiliations

  • J. Jilka
    • 1
  1. 1.Prodigene Inc.TexasUSA

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