Regulated multicistronic expression technology for mammalian metabolic engineering

  • Martin Fussenegger
  • Samuel Moser
  • James E. Bailey
Part of the Current Applications of Cell Culture Engineering book series (CACC, volume 3)


Contemporary basic research is rapidly revealing increasingly complex molecular regulatory networks which are often interconnected via key signal integrators. These connections among regulatory and catalytic networks often frustrate bioengineers as promising metabolic engineering strategies are bypassed by compensatory metabolic responses or cause unexpected, undesired outcomes such as apoptosis, product protein degradation or inappropriate post-translational modification. Therefore, for metabolic engineering to achieve greater success in mammalian cell culture processes and to become important for future applications such as gene therapy and tissue engineering, this technology must be enhanced to allow simultaneous, in cases conditional, reshaping of metabolic pathways to access difficult-to-attain cell states. Recent advances in this new territory of multigene metabolic engineering are intimately linked to the development of multicistronic expression technology which allows the simultaneous, and in some cases, regulated expression of several genes in mammalian cells. Here we review recent achievements in multicistronic expression technology in view of multigene metabolic engineering.

Key words

autoregulation cell-cycle engineering eukaryotic operon IRES multigene engineering picornavirus pTRIDENT regulated expression 


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Copyright information

© Springer Science+Business Media Dordrecht 1998

Authors and Affiliations

  • Martin Fussenegger
    • 1
  • Samuel Moser
    • 1
  • James E. Bailey
    • 1
  1. 1.Institute of BiotechnologySwiss Federal Institute of Technology, ETH ZurichZurichSwitzerland

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