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Correlation between aggregative and Chemotaxis inducing activities of five chemotactic peptides (Abstract)

  • B. Damerau
  • E. Grünefeld
  • W. Vogt
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)

Abstract

Aggregation of leukocytes by chemotactic agents is possibly the in vitro equivalent of leukocyte adherence in vivo, the first step of leukoycte migration to inflammatory sites. We therefore compared the aggregative and the Chemotaxis inducing activity of five chemotactic peptides, namely the complement-derived peptide C3a and C5a and the synthetic N-formyl-methionyl peptides f-Met-Leu-Phe-OH, f-Met-Leu-Leu-OH and f-Met-Leu-OH, using identical cell preparations in both assays. Peripheral human leukocytes were prepared by sedimentation of blood in a dextran-containing medium, suspended in Gey’s solution with 0.5% human serum albumin to a final concentration of 107 cells/ml in the aggregation assay (nephelometric determination in an aggregometer) or to a concentration of 106 cells/ml in the Boyden chamber experiments (filters of 3 µm pore size; leading front method).

Keywords

Human Serum Albumin Leukocyte Adherence Chemotactic Activity Inflammatory Site Total Inhibition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • B. Damerau
    • 1
  • E. Grünefeld
    • 1
  • W. Vogt
    • 1
  1. 1.West Germany

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