Specificity of glucocorticoid inhibition of synovial prostaglandin synthesis

  • R. M. McMillan
  • Constance E. Brinckerhoff
  • Edward D. HarrisJr.
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)


Inhibition of prostaglandin (PG) synthesis contributes to the antiinflammatory activity of a number of anti-rheumatic compounds. Inhibition of PG synthesis by non-steroidal anti-inflammatory drugs (e.g. aspirin, indomethacin) has been observed in experiments with cell-free preparations of PG synthase as well as in intact tissues and cells1–3. These agents appear to act primarily by blocking the enzyme cylo-oxygenase which catalyses the initial step in synthesis of PGs from their fatty acid precursor, arachidonic acid4.


Phorbol Myristate Acetate Synovial Fibroblast Synovial Cell Phorbol Myristate Acetate Fatty Acid Precursor 
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  1. 1.
    Vane, J. R. (1971). Inhibition of prostaglandin synthesis as a mechanism of action for aspirinlike drugs. Nature (London), New Biol., 231, 232Google Scholar
  2. 2.
    Ferreira, S.H., Moncada, S., and Vane, J. R. (1971). Indomethacin and aspirin abolish prostaglandin release from the spleen. Nature (London), New Biol., 231, 237CrossRefGoogle Scholar
  3. 3.
    Smith, J. B. and Willis, A. L. (1971). Aspirin selectively inhibits prostaglandin production in human platelets. Nature (London), New Biol., 231, 235Google Scholar
  4. 4.
    Roth, G. J., Stanford, N., and Majerus, P. W.(1975). Acetylation of prostaglandin synthase by aspirin. Proc. Natl. Acad.Sci. (USA). 72, 3073CrossRefGoogle Scholar
  5. 5.
    Newcombe, D. S., Fahey, J. V. and Ishikawa, Y. (1977). Hydrocortisone inhibition of the bradykinin activation of human synovial fibroblasts. Prostaglandins, 13, 235Google Scholar
  6. 6.
    Tashjian, A.H., Jr., Voelkel, E.F., McDonough, J., and Levine, L. (1975). Hydrocortisone inhibits prostaglandin production by mouse fibrosarcoma cells. Nature (London), 258, 739CrossRefGoogle Scholar
  7. 7.
    Kantrowitz, F., Robinson, D. R., McGuire, M. B., and Levine, L. (1975). Corticosteroids inhibit prostaglandin production by rheumatoid synovia. Nature (London), 258, 737CrossRefGoogle Scholar
  8. 8.
    Blackwell, G. J., Flower, R.J., Nijkamp, R. P., and Vane, J. R. (1978). Phospholipase A2 activity of guineapig isolated perfused lungs: stimulation and inhibition by antiinflammatory steroids. Br. J. Pharmacol, 62, 79PubMedGoogle Scholar
  9. 9.
    Flower, R. J., and Blackwell, G. J. (1979). Anti-inflammatory steroids induce biosynthesis of a phosopholipase A2 inhibitor which prevents prostaglandin generation. Nature (London), 278, 456CrossRefGoogle Scholar
  10. 10.
    Robinson, D. R., Bastian, D. and Servello, L. (1979). Mechanism of corticosteroid inhibition of prostaglandin synthesis by rheumatoid synovial tissue. Arthritis Rheum, 22, 650Google Scholar
  11. 11.
    Danon, A., and Assouline, G. (1978). Inhibition of prostaglandin biosynthesis by corticosteroids requires RNA and protein synthesis. Nature (London), 273, 552CrossRefGoogle Scholar
  12. 12.
    Foley, J. E., Jeffries, M., and Munck, A. (1980). Glucocorticoid effectson incorporation of lipid and protein precursors into rat thymus cell fractions. J. Steroid Biochem. (In press)Google Scholar
  13. 13.
    Jenrenaud, B. and Renold, A. E. (1960). Studies on rat adipose tissue in vitro. VII. Effects of adrenal cortical hormones. J. Biol. Chem., 235, 2217Google Scholar
  14. 14.
    McMillan, R.M., Fahey, J.V., Brinckerhoff, C. E., and Harris, E. D., Jr. (1980). Secretion of inflammatory mediators from synovial fibroblasts: dissociation of collagenase and prostaglandin release. Samuelsson, B., Ramwell, P. W. and Paoletti, R. (eds.) In Advances in Prostaglandin and Thromboxane Research. Vol 8, p 1701. (New York: Raven Press)Google Scholar
  15. 15.
    Hseuh, W., Isakson, P. C. and Needleman, P.(1977). Hormone selective lipase activation in the isolated rabbit heart. Prostaglandins, 13, 1073CrossRefGoogle Scholar
  16. 16.
    Needleman, P. (1978). Characterization of the reaction sequence involved in phospholipid labeling and deacylation and prostaglandin synthesis and actions. J. Allergy Clin. Immunol., 62, 96PubMedCrossRefGoogle Scholar
  17. 17.
    Hong, S.L. and Deykin, D. (1979). Specificity of phospholipases in methylcholantrhene-transformed mouse fibroblasts activated by bradykinin, thrombin, serum and ionophore A23187. J. Biol. Chem., 254, 11463PubMedGoogle Scholar
  18. 18.
    McMillan, R. M.and Harris. E. D., Jr. (unpublished data)Google Scholar

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • R. M. McMillan
    • 1
  • Constance E. Brinckerhoff
    • 1
  • Edward D. HarrisJr.
    • 1
  1. 1.USA

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