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Aggregation of rat polymorphonuclear leukocytes in vitro by chemotactic factors (Abstract)

  • M. E. Shipley
  • F. M. Cunningham
  • M. J. H. Smith
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)

Abstract

Rat leukocytes containing 84 ± 4% polymorphonuclear leukocytes (PMNs) were obtained by peritoneal lavage 24 h after the injection of 6 ml of 12% sodium caseinate. The cells were washed and resuspended at a concentration of 1–1.3 × 107 cells/ml in Eagle’s minimum essential medium buffered to pH 7.4 with 30mmol/l HEPES. The change in particle numbers reflecting aggregation was measured by the decrease in optical density in a Payton aggregometer (37°C, 900 rev/min stirring speed). Both formyl-methionyl-leucyl-phenylalanine (FMLP), over the range 10-5-10-8 mol/1 and zymosan activated serum or plasma, over the range 0.5–10% v/v, produced dose related aggregation responses. The reaction was diminished in the presence of 0.5% human serum albumin and potentiated in the presence of cytochalasin B (optimal concentration 250 ng ml-1). The optimal cell concentration was found to be 1.2 × 107 cells/ml and the technique was highly reproducible (coefficient of variation <8.5%). The aggregation of peripheral rat PMNs in response to FMLP was significantly reduced when compared to that of elicited peritoneal cells.

Keywords

Human Serum Albumin Particle Number Polymorphonuclear Leukocyte Complement Component Peritoneal Lavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • M. E. Shipley
    • 1
  • F. M. Cunningham
    • 1
  • M. J. H. Smith
    • 1
  1. 1.UK

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