Production of hydrogen peroxide from neutrophils of rats (Abstract)
The production-release of hydrogen peroxide (H2O2) from peritoneal neutrophils of rats has been investigated. Cells are elicited by intraperitoneal injections of Mycobacterium butyricum in saline 24 h before cell harvest. Among many other possibilities, phorbol myristate acetate, M. butyricum and Agkistrodonpiscivorus venom are the most effective stimulators of H2O2 production. The various routine kinetic parameters of these reactions have been studied and some will be presented here. Various non-steroidal antiinflammatory drugs have been examined for their effects on H2O2 production and, among these, phenylbutazone is the most potent inhibitor. C21 steroidal anti-inflammatory drugs (6 alpha-methylprednisolone succinate and Cortisol succinate, especially) are potent inhibitors of H2O2 production by stimulated and non-stimulated cells. Arachidonic acid and linolenic acid, among others, are extremely potent inhibitors of H2O2 production-release from stimulated neutrophils and these effects do not appear to be related either to the cyclo-oxygenase initiated or the lipoxygenase initiated ‘cascade’. These and other studies, as well as their probable significance, if any, will be discussed.