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Determination of free lipid A and endotoxin (Abstract)

  • P. C. Fink
  • J. Kozak
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)

Abstract

The wide spectrum of endotoxic activities among the different lipopolysac-charides has been shown recently to reside in the similar antigenic lipid A structure, which is an ubiquitous moiety in bacterial endotoxins (lipo-polysaccharides). Based upon this concept, a heterogeneous enzyme-immunoassay to first determine free lipid A has been set up. Different antisera were obtained from rabbits and goats immunized with lipid A vaccine. The specificity of anti-lipid A IgG antibodies obtained after ammonium sulphate precipitation and DEAE-A-50 chromatography was assayed by passive haemolysis inhibition test.

Keywords

Ammonium Sulphate Positive Blood Culture Sulphate Precipitation Ammonium Sulphate Precipitation Healthy Blood Donor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • P. C. Fink
    • 1
  • J. Kozak
    • 1
  1. 1.W. Germany

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