Quantitative measurement of human leukocyte aggregation as a possible correlation with cell mediated immunity

  • B. Rouveix
  • P. Badenoch-Jones
  • J. L. Turk
Part of the Inflammation: Mechanisms and Treatment book series (FTIN, volume 4)


The study of cell mediated immunity has been facilitated by the development of in vitro methods, especially in experimental animals. However, there are no wholly satisfactory in vitro methods for use with humans. Inhibition of migrating indicator cells resulting from the interaction of sensitized lymphocytes with specific antigen and the lymphocyte transformation test (LTT) are widely used. Both of these techniques have been extensively studied and compared with in vivo delayed hypersensitivity reactions, as assessed by skin testing1–5. They both have disadvantages. The migration inhibition is cumbersome, difficult to set up and is sometimes no better than semi-quantitative. In the lymphocyte transformation test, stimulation of antibody-producing cells by antigen may also be measured. It is not surprising, therefore, that the results obtained with these tests are not always correlated with each other and with skin test results1,5–7. The direct migration inhibition of leukocytes from sensitized subjects by specific antigen is thought to be due to release of lymphognes (leukocyte inhibitory factor, LIF) from lymphocytes which inhibits the spontaneous migration of mainly polymorphonuclear leukocytes. Indirect migration inhibition can be measured by assaying culture supernatants of sensitized lymphocytes and specific antigen on the migration of normal leukocytes.


Migration Inhibition Factor Cell Mediate Immunity Peritoneal Exudate Cell Macrophage Aggregation Migration Inhibition 
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  1. 1.
    Fleer, A., Van der Hart, M., Blok-Shut, B. J.T.H. and Schellekens, P.T.H. A. (1976). Correlation of PPD and BCG-induced leukocyte migration inhibition, delayed cutaneous sensitivity, lymphocyte transformation in vitro and humoral antibodies to PPD in man. Eur. J. Immunol., 6, 163PubMedCrossRefGoogle Scholar
  2. 2.
    Marsman, A. J. W., Van der Hart, M., Walig, C. and Eijsvoogel, V. P. (1972). Migration inhibition experiments with mixtures of human peripheral blood lymphocytes and guinea pig peritoneal exudate cells. Eur. J. Immunol., 2, 546.PubMedCrossRefGoogle Scholar
  3. 3.
    Mitchell, C. G., Smith, M. G. H., Golding, P. L., Eddleston, A. L. W. F., and Williams, R. (1972). Evaluation of the leukocyte migration inhibition test as a measure of delayed hypersensitivity in man. Suppression of migration inhibition by puromycin. Clin. Exp. Immunol., 11, 535PubMedGoogle Scholar
  4. 4.
    Rosenberg, S. A. and David, J. R. (1970). Inhibition of leukocyte migration: an evaluation of this in vitro assay of delayed hypersensitivity in man to a soluble antigen. J. Immunol., 105, 1447Google Scholar
  5. 5.
    Thomas, J. H., Clements, D. and Grzybowski, S. (1971). In vitro lymphocyte responses and skin test reactivity following BCG vaccination. Clin. Exp. Immunol., 9, 611PubMedGoogle Scholar
  6. 6.
    Kaltreider, H.B., Soghar, D., Taylor, J. B. and Decker, J. L. (1969). Capillary tube migration for detection of human delayed hypersensitivity: difficulties encountered with “buffy coat” cells and tuberculin antigen. J. Immunol., 103, 179PubMedGoogle Scholar
  7. 7.
    Locksin, M.D., (1969). Failure to demonstrate leukocyte migration inhibition in human tuberculin sensitivity. Proc. Soc. Exp. Biol. Med., 132, 928Google Scholar
  8. 8.
    Bloom, B.R. (1971). In Bloom, B.R. and Glade, P. R. (eds.) In vitro methods in cell-mediated immunity, pp. 3–93. (New York: Academic Press)Google Scholar
  9. 9.
    Lolekha, S., Dray, S. and Gotoff, S. P. (1970). Macrophage aggregation in vitro: a correlate with delayed hypersensitivity. J. Immunol., 104, 296PubMedGoogle Scholar
  10. 10.
    Gotoff, S. P., Vizralj, I.F. and Malecki, T. J. (1970). Macrophage aggregation in vitro: a model for transplantation immunity. Transplantation, 10, 433PubMedCrossRefGoogle Scholar
  11. 11.
    Gotoff, S. P., Lolekha, S., Lopata, M., Kopp, J., Kopp, R.L., and Malecki, T.J. (1973). The macrophage aggregation assay cell mediated immunity in man. Studies on patients with Hodgkin’s disease and sarcoidosis. J. Lab. Clin. Med., 82, 682PubMedGoogle Scholar
  12. 12.
    Ford, P. M., Ford, S. E. and Gibson, G. (1977). Evaluation of antigen-induced buffy coat leukocyte aggregation as a simple test for allergic reactivity. Int. Arch. Allergy Appl. Immunol., 53, 56PubMedCrossRefGoogle Scholar
  13. 13.
    Nicholls, E. M. (1974). Aggregation of buffy coat leukocytes. A simple sensitive assay for cell mediated immunity. Clin. Exp. Immunol., 17, 673PubMedGoogle Scholar
  14. 14.
    Rouveix, B., Badenoch-Jones, P. and Turk, J. L. (1979). A sensitive technique for measuring macrophage aggregation: a comparison with macrophage migration inhibition for the detection of lymphokine activity. Immunology, 36, 589PubMedGoogle Scholar
  15. 15.
    Badenoch-Jones, P., Rouveix, B. and Turk, J. L. (1980). Studies on lymphokine-induced macrophage aggregation. Specificity and quantitative aspects. Int. Arch. Allergy Appl. Immunol., 62, 188PubMedCrossRefGoogle Scholar

Copyright information

© MTP Press Limited 1980

Authors and Affiliations

  • B. Rouveix
    • 1
  • P. Badenoch-Jones
    • 2
  • J. L. Turk
    • 2
  1. 1.France
  2. 2.UK

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