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Influence of Hg2+ on the Excited States of DNA: Photochemical Consequences

  • Ronald O. Rahn
Conference paper
Part of the The Jerusalem Symposia on Quantum Chemistry and Biochemistry book series (JSQC, volume 10)

Abstract

Metal ions have been widely used as probes in excited-state studies of nucleic acids. One of the first demonstrations of triplet energy transfer in poly(A) and DNA was made by Bersohn and Eisenberg (1964), using Mn2+ as a triplet state quencher. These observations were extended by Eisinger and Shulman (1966) to include Co2+, Ni2+, and Cu2+ all of which were shown to quench long-range triplet transfer in poly(A) without quenching the fluorescence. Hélène and co-workers (see Hélène, 1973) showed that similar quenching mechanisms operated in adenosine aggregates but that, in addition, Cu+2 quenched the adenosine fluorescence by singlet transfer to an adenosine-Cu2+ complex. Energy transfer methods, employing europium ions as energy traps have also been used to study fluorescence lifetimes and intersystem crossing yields of nucleic acid monomers at 25°C (Lamola and Eisinger, 1971).

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References

  1. Bersohn, R. and Isenberg, I. (1963) Biochem. Biophys. Res. Coiranun., 13, 205CrossRefGoogle Scholar
  2. Chen, R. F. (1971) Arch. Biochem. Biophys. 142, 552CrossRefGoogle Scholar
  3. Eisinger, J. (1966) in Electron Spin Resonance and the Effects of Radiation on Biological Systems (Snipes, W., ed), p. 76, National Academy of Sciences, WashingtonGoogle Scholar
  4. Eisinger, J. and Shulman, R. G. (1966) Proc. Natl. Acad. Sci. U.S.A. 55, 1387CrossRefGoogle Scholar
  5. Hauswirth, W. (1971) Thesis, Oregon State UniversityGoogle Scholar
  6. Helene,C. (1973) in Physico-chemical Properties of Nucleic Acids, Vol. 1 (Duchesne, J., ed.), p. 119, Academic Press, New YorkGoogle Scholar
  7. Katz, S. (1963) Biochim. Biophys. Acta 68, 240CrossRefGoogle Scholar
  8. Lamola, A. A. and Eisinger, J. (1971) Biochim. Biophys. Acta 240, 313CrossRefGoogle Scholar
  9. Munchausen, L. L. and Rahn, R. O. (1975) Biochim. Biophys. Acta 414, 242CrossRefGoogle Scholar
  10. Rahn, R. O. and Landry, L. C. (1970) Proc. Natl. Acad. Sci. U.S.A. 67, 1390Google Scholar
  11. Rahn, R. O. and Landry, L. C. (1971) Biochim. Biophys. Acta 247, 197CrossRefGoogle Scholar
  12. Rahn, R. O. and Landry, L. C. (1973) Photochem. Photobiol. 18, 20Google Scholar
  13. Sutherland, B. M. and Sutherland, J. C. (1969a) Biophys. J. 9, 1329CrossRefGoogle Scholar
  14. Yamane, T. and Davidson, N. (1961) J. Amer. Chem. Soc. 83, 2599CrossRefGoogle Scholar
  15. Yamane, T. and Davidson, N. (1962) Biochim. Biophys. Acta 55, 780CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1977

Authors and Affiliations

  • Ronald O. Rahn
    • 1
  1. 1.Biology DivisionOak Ridge National LaboratoryOak RidgeUSA

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