Influence of Hg2+ on the Excited States of DNA: Photochemical Consequences
Metal ions have been widely used as probes in excited-state studies of nucleic acids. One of the first demonstrations of triplet energy transfer in poly(A) and DNA was made by Bersohn and Eisenberg (1964), using Mn2+ as a triplet state quencher. These observations were extended by Eisinger and Shulman (1966) to include Co2+, Ni2+, and Cu2+ all of which were shown to quench long-range triplet transfer in poly(A) without quenching the fluorescence. Hélène and co-workers (see Hélène, 1973) showed that similar quenching mechanisms operated in adenosine aggregates but that, in addition, Cu+2 quenched the adenosine fluorescence by singlet transfer to an adenosine-Cu2+ complex. Energy transfer methods, employing europium ions as energy traps have also been used to study fluorescence lifetimes and intersystem crossing yields of nucleic acid monomers at 25°C (Lamola and Eisinger, 1971).
Unable to display preview. Download preview PDF.
- Eisinger, J. (1966) in Electron Spin Resonance and the Effects of Radiation on Biological Systems (Snipes, W., ed), p. 76, National Academy of Sciences, WashingtonGoogle Scholar
- Hauswirth, W. (1971) Thesis, Oregon State UniversityGoogle Scholar
- Helene,C. (1973) in Physico-chemical Properties of Nucleic Acids, Vol. 1 (Duchesne, J., ed.), p. 119, Academic Press, New YorkGoogle Scholar
- Rahn, R. O. and Landry, L. C. (1970) Proc. Natl. Acad. Sci. U.S.A. 67, 1390Google Scholar
- Rahn, R. O. and Landry, L. C. (1973) Photochem. Photobiol. 18, 20Google Scholar