The Neutralisation of the Indirect Immunofluorescence Test and the Immunodiffusion Test: Two Detection Methods for Neonatal Calf Diarrhoea Viruses. A Comparative Study with Elisa and Electron Microscopy

  • E. Van Opdenbosch
  • G. Wellemans
  • D. J. Ellens
  • P. W. De Leeuw
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)


A comparative study of the neutralisation of the indirect immunofluorescence (NIIF) test, the immunodiffusion (ID) tes, enzyme-linked immunosorbent assay (ELISA) and electron microscopy (EM) for the detection of rotaviruses in calf faeces was done at the Nationaal Instituut voor Diergeneeskundig Onderzoek (NIDO) in Belgium and the Centraal Diergeneeskundig Instituut (CDI) in The Netherlands. A total of 93 faecal samples was examined. Sixty-two per cent was positive for rotavirus by EM, 59% with the NIIF test, 58% with the ID test and 57% with ELISA. All samples negative by EM were also negative in the NIIF and the ID test but three of these samples were found positive in the ELISA.

Forty-three faecal samples were tested for the presence of bovine Coronavirus. Eleven samples were scored positive by EM of which ten were positive in the NIIF test and five in ELISA. Two other samples were found positive by ELISA only.


Faecal Sample Neonatal Calf Healthy Calf Calf Diarrhoea Bovine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. 1.
    Ellens, D.J., De Leeuw, P.W.: An enzyme linked immunosorbent assay (ELISA)for the diagnosis of rotavirus infections in calves. J.Clin. Microbiol. 6: 530–532, 1977.PubMedGoogle Scholar
  2. 2.
    Ellens, D.J., De Leeuw, P.W., Straver, P.J. and Van Balken, J.A.M.: Comparison of five diagnostic methods for the detection of rotavirus antir gens in calf faeces. Med.Mierob.Immunol. 166: 157–163, 1978.CrossRefGoogle Scholar
  3. 3.
    Ellens, D.J., Van Balken, J.A.M. and De Leeuw, P.W.: Diagnosis of bovine coronavirus infections with hemadsorption-elution-hemagglutination assay (HEMA) and with enzyme-linked immunosorbent assay (ELISA).Proc. Second Int.Symp. on Neonatal Diarrhea, University of Saskatchewan, Canada, p.322–329, 1978.Google Scholar
  4. 4.
    Scherrer, B.S.: Application d’une technique immunoenzymologique (ELISA) à la détection du rotavirus bovin et des anticorps dirigés contre lui. Ann.Microbiol. (Institut Pasteur) 128A: 499–519, 1977.Google Scholar
  5. 5.
    Van Opdenbosch, E., Dekegel, D. and Wellemans, G.: De immunodiffusietest voor het opsporen van het Rotavirus in meststalen en darmfragmenten. Vl.Dierg. Tijdschrift 47: 276–291, 1978.Google Scholar
  6. 6.
    Wellemans, G., Van Opdenbosch, E., et Dekegel, D.: La neutralisation de l’immunofluorescence indirecte (NIFI): une technique spécifique et quantitative pour la mise en évidence d’antigènes viraux. Ann.Med.Vét. 123: 185–194, 1979.Google Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • E. Van Opdenbosch
    • 1
  • G. Wellemans
    • 1
  • D. J. Ellens
    • 2
  • P. W. De Leeuw
    • 2
  1. 1.Nationaal Instituut voor Diergeneeskundig OnderzoekBrusselBelgium
  2. 2.Centraal Diergeneeskundig InstituutLelystadThe Netherlands

Personalised recommendations