Diagnosis by Enzyme-Linked Immunosorbent Assay
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A variety of enzyme-linked immunosorbent assay (ELISA) systems are being applied in the detection and quantification of both antibodies and antigens in bacterial, viral and parasitic infections. To date, the use of ELISA in research on neonatal diarrhoea has been described for the detection of rotavirus, bovine coronavirus and enteropathogenic Escherichia coli infections.
Some features of the double antibody sandwich ELISA system are discussed with particular reference to those elements most important in determining the specificity and sensitivity of the test.
KeywordsHorse Radish Peroxidase Double Antibody Sandwich Alkaline Phosphatase Substrate Candida Antigen Bovine Coronavirus
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