Diagnosis by Enzyme-Linked Immunosorbent Assay

  • Daniel J. Ellens
Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)


A variety of enzyme-linked immunosorbent assay (ELISA) systems are being applied in the detection and quantification of both antibodies and antigens in bacterial, viral and parasitic infections. To date, the use of ELISA in research on neonatal diarrhoea has been described for the detection of rotavirus, bovine coronavirus and enteropathogenic Escherichia coli infections.

Some features of the double antibody sandwich ELISA system are discussed with particular reference to those elements most important in determining the specificity and sensitivity of the test.


Horse Radish Peroxidase Double Antibody Sandwich Alkaline Phosphatase Substrate Candida Antigen Bovine Coronavirus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Berg, R.A., Rennard, S.I., Murphy, B.R., Yolken, R.H., Dolin, R. and Straus, S.E.: New enzyme immunoassays for measurement of influenza A/Victoria/3/75/virus in nasal washes. The Lancet, vol. I: 851–853, 1980.CrossRefGoogle Scholar
  2. Clark, M.F. and Adams, A.N.: Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34: 475–483, 1977.PubMedCrossRefGoogle Scholar
  3. De Leeuw, P.W., Ellens, D.J., Straver, P.J., Van Balken, J.A.M., Moerman, A. and Baanvinger, Th.: Rotavirus infection in calves in dairy herds. Res.Vet.Sci. 29: 135–141, 1980.PubMedGoogle Scholar
  4. Ellens, D.J., De Leeuw, P.W., Straver, P.J. and Van Balken, J.A.M.: Comparison of five diagnostic methods for the detection of rotavirus antigens in calf faeces. Med.Microbiol.Immunol. 166: 157–163, 1978.PubMedCrossRefGoogle Scholar
  5. Engvall, E. and Perlman, P.: Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 8: 871, 1971.PubMedCrossRefGoogle Scholar
  6. Harris, C.C., Yolken, R.H., Krokan, H. and Hsu, I.C.: Ultrasensitive enzymatic radioimmunoassay: Application to detection of cholera toxin and rotavirus. Proc. Natl. Acad. Sci. U.S.A. 76: 5336–5339, 1979.PubMedCrossRefGoogle Scholar
  7. Kalica, A.R., Purcell, R.H., Sereno, M.M., Wyatt, R.G., Kim, H.W., Channock, R.M. and Kapikian, A.J.: A microtiter solid phase radioimmunoassay for the detection of the human reovirus-like agent in stools. J.Immun. 118: 1275–1279, 1977.PubMedGoogle Scholar
  8. Norde, W.: Adsorption of proteins at solid surfaces. In: Adhesion and adsorption of polymers. Polymer science and technology, vol. 12B, Plenum Press, N.Y., Lieng Huang Lee ed., p. 801, 1980.Google Scholar
  9. Ruitenberg, E.J., Van Amstel, J.A., Brosi, B.J.M. and Steerenberg, P.A.: Mechanization of the enzyme-linked immunosorbent assay (ELISA) for large scale screening of sera. J.Immunol.Meth. 16: 351–359, 1977.CrossRefGoogle Scholar
  10. Segal, E., Berg, R.A., Pizzo, P. and Bennett, J.E.: Detection of candida antigen in sera of patients with candiasis by an enzyme-linked immunosorbent assay-inhibition technique. J.Clin.Microbiol. 10: 116–119, 1979.PubMedGoogle Scholar
  11. Svennerholm, A.M. and Holmgren, J.: Identification of E. coli, heat-labile enterotoxin (LT) by means of a ganglioside immunosorbent assay (Gm1-ELISA). Current Microbiology, 1: 19–24, 1978.CrossRefGoogle Scholar
  12. Van Weemen, B.K. and Schuurs, A.H.W.M.: Immunoassay using antigen-enzyme conjugates. FEBS Lett. 15: 232–236, 1971.PubMedCrossRefGoogle Scholar
  13. Wilson, B. and Nakane, P.K.: Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies. In: Immunofluorescence and related staining techniques. Elsevier/North-Holland Biomedical Press. W. Knapp, K. Holubar and G. Wicks, eds., p. 215, 1978.Google Scholar
  14. Yolken, R.H., Barbour, B., Wyatt, R.G., Kalica, A.R., Kapikian, A.Z. and Channock, R.M.: Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science 201: 259–262, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • Daniel J. Ellens
    • 1
  1. 1.Department of VirologyCentral Veterinary InstituteLelystadthe Netherlands

Personalised recommendations