Laboratory manual of the Central Veterinary Institute, Virology Department, 8221 RA Lelystad, The Netherlands
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A polystyrene solid phase is coated with antibodies directed against bovine-rotavirus antigens. Upon addition of a faecal extract, rotavirus antigens (if present) will be trapped by specific antibodies covalently linked to the enzyme peroxidase (conjugate). The bound conjugate is visualized through addition of a chromogenic substrate. Specificity of the reaction is checked by blocking the conjugate reaction with a specific anti-rotavirus serum.