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Station de Recherches de Virologie et d’Immunologie Inra, 78850-Thiverval-Grignon, France

  • R. Scherrer
  • Chantal Feynero
  • R. L’Haridon
Chapter
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Part of the Current Topics in Veterinary Medicine and Animal Science book series (CTVM, volume 13)

Abstract

The wells in a polystyrene plate (solid phase) are coated with a specific anti rotavirus Ig preparation (sensitization of the wells). Plates are then washed in order to remove immunoglobulins that are not adsorbed. Upon addition of faecal extracts, rotavirus particles and/or antigens, if they are present, will be trapped by the immobilized antibodies. After removal of unbound material, (washing) a conjugate consisting of antirotavirus antibodies coupled to alkalin phosphatase, is added in the wells; this conjugate will react with any rotaviral antigen already trapped in the wells. A further washing removes excess conjugate and finally, the bound conjugate is visualized by adding the enzyme substrate (p-nitrophenylphosphate).

Keywords

Polystyrene Plate Faecal Extract Rotaviral Antigen Porcin Milk Positive Reference Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© ECSC, EEC, EAEC, Brussels-Luxembourg 1981

Authors and Affiliations

  • R. Scherrer
    • 1
  • Chantal Feynero
    • 1
  • R. L’Haridon
    • 2
  1. 1.Station de Recherches de Virologie et d’Immunologie INRAThiverval-GrignonFrance
  2. 2.Laboratoire de Pathologie du Bétail ENVMaisons-AlfortFrance

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