Station de Recherches de Virologie et d’Immunologie Inra, 78850-Thiverval-Grignon, France
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The wells in a polystyrene plate (solid phase) are coated with a specific anti rotavirus Ig preparation (sensitization of the wells). Plates are then washed in order to remove immunoglobulins that are not adsorbed. Upon addition of faecal extracts, rotavirus particles and/or antigens, if they are present, will be trapped by the immobilized antibodies. After removal of unbound material, (washing) a conjugate consisting of antirotavirus antibodies coupled to alkalin phosphatase, is added in the wells; this conjugate will react with any rotaviral antigen already trapped in the wells. A further washing removes excess conjugate and finally, the bound conjugate is visualized by adding the enzyme substrate (p-nitrophenylphosphate).
KeywordsPolystyrene Plate Faecal Extract Rotaviral Antigen Porcin Milk Positive Reference Serum
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