Advertisement

Chlorophyll Fluorescence Signatures as Vitality Indicator in Forest Decline Research

  • Hartmut K. Lichtenthaler
  • Ursula Rinderle

Abstract

The chlorophyll-fluorescence signatures of the needles of healthy (damage class 0/1, Althof, 450 m above sea level) and damaged spruce trees (damage class 3/4, Mauzenberg 700 m altitude) in the northern Black Forest were determined by using a self-constructed portable field fluorometer as well as the PAM-fluorometer. These new instruments allow measurement of the red-laser-induced chlorophyll fluorescence induction kinetics (Kautsky effect) at 690 and 730 nm, the determination of the Rfd-values (Rfd 690 and Rfd 730) as vitality index, and the stress-adaptation index Ap as well as the photochemical Q-quenching and the non-photochemical E-quenching of the spruce needles. The differences in the fluorescence signatures and photosyn-thetic activity between the needles of healthy and damaged trees are presented. In the needles of the damaged trees the Rfd-values, the capacity for photosynthesis and the reoxidation of QA are strongly reduced, whereas the non-photochemical E-quenching is increased.

The chlorophyll fluorescence is a very powerful non-destructive method for fast outdoor screening of tree physiology and for ground-truth measurements in remote sensing of forest decline as well as detection of damage to the photosynthetic apparatus.

Key Words

Chlorophyll fluorescence forest decline Kautsky effect PAM-fluorometer photosynthesis Rfd-values vitality index 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. LICHTENTHALER HK, 1984. Luftschadstoffe als Auslöser des Baumsterbens. Naturwiss. Rundschau 37: 271–277.Google Scholar
  2. LICHTENTHALER HK, 1986. Laser-induced chlorophyll fluorescence of living plants. In: Proc. Internat. Geoscience and Remote Sensing Symposium IGARSS Zürich 1986, Vol. III, pp. 1571–1579, ESA Publications Division, Noordwijk.Google Scholar
  3. LICHTENTHALER HK, 1987. Chlorophylls and carotenoids, the pigments of photosynthetic biomembranes. Methods in Enzymology: 148, 350–382.CrossRefGoogle Scholar
  4. LICHTENTHALER HK and BUSCHMANN C, 1984a. Beziehungen zwischen Photosynthese und Baumsterben. Allgem. Forstzeitschrift 39: 12–16.Google Scholar
  5. LICHTENTHALER HK, and BUSCHMANN C, 1984b. Das Waldsterben aus botanischer Sicht. G. Braun Verlag, Karlsruhe.Google Scholar
  6. LICHTENTHALER HK and RINDERLE U, 1988. The role of chlorophyll fluorescence in the detection of stress conditions in plants. CRC Critical Reviews in Analytical Chemistry 19, Suppl. I: S 29–S 85.Google Scholar
  7. LICHTENTHALER HK, SCHMUCK G, DOLL M and BUSCHMANN C, 1985. Untersuchungen über die Funktionsfähigkeit des Photosyntheseapparates bei Nadeln gesunder und geschädigter Koniferen. PEF-Bericht KfK-PEF 2, PEF Projektleitung ed., pp. 81–105, Kernforschungszentrum Karlsruhe.Google Scholar
  8. LICHTENTHALER HK, BUSCHMANN C, RINDERLE U and SCHMUCK G, 1986. Application of chlorophyll fluorescence in ecophysiology. Radiation and Environmental Biophysics 25: 297–308.CrossRefPubMedGoogle Scholar
  9. NAGEL EM, BUSCHMANN C and LICHTENTHALER HK, 1987. Photoacoustic spectra of needles as an indicator for the activity of the photosynthetic apparatus of healthy and damaged conifers. Physiol. Plantarum 70: 427–437.CrossRefGoogle Scholar
  10. SCHMUCK G and LICHTENTHALER HK, 1986. Application of laser-induced chlorophyll a fluorescence in the forest decline research. In: Proc. Internat. Geoscience and Remote Sensing Symposium IGARSS Zürich 1986, Vol. III, pp. 1587–1590, ESA Publications Division, Noordwijk.Google Scholar
  11. SCHREIBER U, 1986. Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth. Res.: 9, 261–272.CrossRefPubMedGoogle Scholar
  12. SCHREIBER U, SCHLIWA U and BILGER W, 1986. Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10: 51–62.CrossRefPubMedGoogle Scholar
  13. STRASSER R, SCHWARZ B and BUCHER J, 1987. Simultane Messung der Chlorophyll Fluoreszenz-Kinetik bei verschiedenen Wellenlängen als rasches Verfahren zur Frühdiagnose von Immissionsbelastungen an Waldbäumen. Ozoneinwirkung auf Buchen und Pappeln. European Journal of Forest Pathology: 17, 149–157.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Hartmut K. Lichtenthaler
    • 1
  • Ursula Rinderle
    • 1
  1. 1.Botanisches Institut II (Plant Physiology and Plant Biochemistry)University of KarlsruheKarlsruheGermany

Personalised recommendations