Porcine-murine heterohybridomas as stabb fusion partners for the production of porcine IgA
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The immunoprophylaxis of neonates against intestinal pathogens remains a challenge. The approach is generally based on the entero-mammary linkage of the gestationing animal. However, a clear correlation between the virulence of the pathogen and IgA inducing capability exists. Therefore, our investigation aimed at the possibility of inducing mucosal immunity via the anti-idiotype pathway. Recent advances in human MAB production (for review Teng, N.N. 1985) and IgA producing hybridomas (Colwell, D., 1986, Dean, C., 1986) made research on this concept in a veterinary species feasible. PM-1 is a stable, non-Ig producing murine-porcine heteromyeloma capable of high fusion rates and stable porcine MAB production upon fusion with porcine lymphocytes of various sources (triple heterohybrids). PM-1 was obtained by intensive subcloning, in selective medium, of hybridomas resulting from the fusion of the murine myeloma P3x63-Ag8.653 and the porcine lymphoblastoid cell line P-16. Fusions of PM-1 with PBL, MNL, TDL and SL resulted fusion rates of 20–100% (hybridomas/wells inoculated). A total of 359 hybridomas were monoclonal Ig-producers. The majority of those analysed produced the IgM isotype. Only one heteorhybrid, YF 42-1, resulting from a PM-1 x PBL fusion was identified as producing dimeric IgA. Fusion with TDL, obtained from pigs after surgical removal of the mesenteric lymph nodes, resulted in 16 IgM and 2 IgG producing hybridomas. No IgA producing hybridomas were obtained from PM-1 x TDL fusions.
KeywordsMesenteric Lymph Node Fusion Rate Intestinal Lymph High Fusion Rate Double Minute Chromosome
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