Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

  • Agnieszka KaczorEmail author
  • Marta Pilarczyk
Part of the Challenges and Advances in Computational Chemistry and Physics book series (COCH, volume 14)


Carotenoids are particularly convenient subjects to study by means of Raman spectroscopy due to very high Raman scattering cross-section associated with their chain vibrations. Raman studies of carotenoids in single cells have a variety of applications starting from monitoring of growth and morphogenesis of unicellular algae and ending with differentiation of cancerous versus non-cancerous tissues in humans. Examples illustrating the potential of Raman spectroscopy to investigate carotenoids structure and distribution with a particular impact on studies using chemometric and computational methods as a tool to analyze experimental data are reviewed in this chapter.


carotenoids Raman imaging chemometrics quantum-chemical calculations unicellular algae 


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© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Faculty of ChemistryJagiellonian UniversityKrakowPoland
  2. 2.Jagiellonian Centre of Experimental Therapeutics (JCET)Jagiellonian UniversityKrakowPoland

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