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Patch-Clamp Measurements of Elementary Chloride Currents Activated by the Putative Inhibitory Transmitters GABA and Glycine in Mammalian Spinal Neurons

  • B. Sakmann
  • O. P. Hamill
  • J. Bormann
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 18)

Summary

Methods for measuring whole-cell and unitary Cl currents in the soma membrane of cultured spinal cord neurons by the patch-clamp technique are described. To separate single channel Cl currents activated by the two putative inhibitory transmitters GABA and glycine from other membrane currents, isotonic KCl pipette solutions are used for measurements on “cell-attached” membrane patches. To isolate unitary Cl currents in “cell-free” membrane patches, symmetrical, isotonic Tris Cl solutions are used in both the pipette and the bath. Single Cl channels are opened following binding of two agonist molecules to their respective receptors. The gating behavior of single receptor-channel complexes is described in terms of the activation and desensitization mechanism suggested originally by del Castillo and Katz (1957) and Katz and Thesleff (1957).

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Copyright information

© Springer-Verlag Wien 1983

Authors and Affiliations

  • B. Sakmann
    • 2
  • O. P. Hamill
    • 1
  • J. Bormann
    • 1
  1. 1.Max-Planck-Institut für biophysikalische ChemieGöttingenFederal Republic of Germany
  2. 2.Max-Planck-Institut für biophysikalische ChemieGöttingenFederal Republic of Germany

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