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5-Hydroxytryptamine Receptors in Neurones and Glia

  • G. Fillion
  • Dominique Beaudoin
  • Maria-Paule Fillion
  • J.-C. Rousselle
  • Christine Robaut
  • Y. Netter
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 18)

Summary

Crude membranal fractions isolated from mammalian brain tissue contain two classes of recognition sites capable of binding [3H]5-HT with high affinity constants.

These classes of sites are characteristics of the postsynaptosomal membrane fraction for the higher affinity and of a glial cell membrane fraction for the lowest. They are observed with similar properties in cultured neuronal and glial cell respectively. Two 5-HT stimulated adenylate cyclases are present in crude membrane fraction; they are also separable as neuronal and glial components.

These observations correspond likely to the existence of two classes of receptors for 5-HT. Their mechanisms of regulation involve presumably structural conformation changes of the recognition site coupled to various states of the activity of the receptor.

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Copyright information

© Springer-Verlag Wien 1983

Authors and Affiliations

  • G. Fillion
    • 1
  • Dominique Beaudoin
    • 2
  • Maria-Paule Fillion
    • 2
  • J.-C. Rousselle
    • 2
  • Christine Robaut
    • 2
  • Y. Netter
    • 3
  1. 1.Unit of Pharmacology, Department of Experimental PhysiopathologyPasteur InstituteParisFrance
  2. 2.Department of PharmacologyPasteur InstituteParisFrance
  3. 3.Laboratoire de Biochimie CellulaireParisFrance

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