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Serotonin Binding in Rat Brain: Circadian Rhythm and Effect of Sleep Deprivation

  • W. Wesemann
  • N. Weiner
  • M. Rotsch
  • E. Schulz
Conference paper
Part of the Journal of Neural Transmission book series (NEURAL SUPPL, volume 18)

Summary

Crude membrane fractions isolated from rat brain were used to measure specific serotonin (5-HT) binding. In untreated animals a 24 hours rhythm in 5-HT binding was evident. Sleep deprivation (SD) was applied to enhance sleep pressure. After 12, 24, and 72 hours SD the high affinity 5-HT binding, KD1 = 1−3 nM, remained unchanged, the apparent dissociation constant of the low affinity 5-HT binding, KD2 = 20−30 nM, was found to be enhanced almost by factor 2. The number of binding sites were only moderately affected by SD. If SD was followed by 1 or 4 hours recovery sleep, the dissociation constant KD2 was decreased towards control values.

The brain levels of 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) were not significantly changed immediately after 72 hours SD. A statistically significant increase was found only for 5-HIAA 4 hours after 72 hours SD.

The results are consistent with the hypothesis that changes in the sensitivity of 5-HT binding may be involved in the regulation of the sleep-waking cycle.

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Copyright information

© Springer-Verlag Wien 1983

Authors and Affiliations

  • W. Wesemann
    • 1
  • N. Weiner
    • 2
  • M. Rotsch
    • 2
  • E. Schulz
    • 2
  1. 1.Abteilung Neurochemie, Physiologisch-Chemisches Institut IIPhilipps-UniversitätMarburg/LahnFederal Republic of Germany
  2. 2.Abteilung Neurochemie, Physiologisch-Chemisches Institut IIPhilipps-UniversitätMarburgFederal Republic of Germany

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