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In-vitro-Fertilisation und intrazytoplasmatische Spermieninjektion

  • Thomas EbnerEmail author
  • Klaus Diedrich
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Zusammenfassung

Konnten anfänglich nur Paare mittels konventioneller IVF behandelt werden, bei denen eine tubare oder unerklärte Sterilität vorlag, erlaubte die Entwicklung invasiverer Befruchtungstechniken (z. B. der ICSI) letztendlich auch eine Therapie bei ausgeprägtem männlichem Faktor. Erst nach der Gewinnung der Cumulus-Eizell-Komplexe wird anhand der Samenqualität des Partners entschieden, welche Technik angewandt wird. Die herkömmliche IVF ist im Wesentlichen der in vivo stattfindenden Befruchtung nachempfunden, d. h., in einem Kulturschälchen werden die Eizellkomplexe mit einer festgelegten Zahl an Spermien versetzt und traditionellerweise über Nacht inseminiert. Bei einer ICSI hingegen müssen vor der geplanten Injektion die die Eizelle umgebenden Cumuluszellen enzymatisch/mechanisch entfernt werden. Das mittels unterschiedlichster Präparationstechniken gewonnene Spermium muss vor Verwendung immobilisiert werden.

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Literatur

  1. Abdelmassih S, Cardoso J, Abdelmassih V, Dias JA, Abdelmassih R, Nagy ZP (2002) Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates. Hum Reprod 17:2694–2699PubMedCrossRefGoogle Scholar
  2. Aktan TM, Montag M, Duman S, Gorkemli H, Rink K, Yurdakul T (2004) Use of a laser to detect viable but immotile spermatozoa. Andrologia 36:366–369PubMedCrossRefGoogle Scholar
  3. Balaban B, Lundin K, Morrell JM, Tjellström H, Urman B, Holmes PV (2003) An alternative to PVP for slowing sperm prior to ICSI. Hum Reprod 18:1887–1889PubMedCrossRefGoogle Scholar
  4. Baltaci V, Ayvaz OU, Unsal E, Aktaş Y, Baltacı A, Turhan F, Ozcan S, Sönmezer M (2010) The effectiveness of intracytoplasmic sperm injection combined with piezoelectric stimulation in infertile couples with total fertilization failure. Fertil Steril 94:900–904PubMedCrossRefGoogle Scholar
  5. Barak Y, Menezo Y, Veiga A, Elder K (1999) A physiological replacement for polyvinylpyrrolidone (PVP) in assisted reproductive technology. Hum Fertil 4:99–103CrossRefGoogle Scholar
  6. Bartoov B, Berkovitz A, Eltes F, Kogosovsky A, Yagoda A, Lederman H, Artzi S, Gross M, Barak Y (2003) Pregnancy rates are higher with intracytoplasmic morphologically selected sperm injection than with conventional intracytoplasmic injection. Fertil Steril 80:1413–1419PubMedCrossRefGoogle Scholar
  7. Bedaiwy MA, Falcone T, Mohamed MS, Aleem AA, Sharma RK, Worley SE, Thornton J, Agarwal A (2004) Differential growth of human embryos in vitro: role of reactive oxygen species. Fertil Steril 82:593–600PubMedCrossRefGoogle Scholar
  8. Blake M, Garrisi J, Tomkin G, Cohen J (2000) Sperm deposition site during ICSI affects fertilization and development. Fertil Steril 73:131–137CrossRefGoogle Scholar
  9. Boitrelle F, Ferfouri F, Petit JM, Segretain D, Tourain C, Bergere M, Bailly M, Vialard F, Albert M (2011) Large human sperm vacuoles observed in motile spermatozoa under high magnification: nuclear thumbprints linked to failure of chromatin condensation. Hum Reprod 26:1650–1658PubMedCrossRefGoogle Scholar
  10. Boitrelle F, Albert M, Petit JM, Ferfouri F, Wainer R, Bergere M, Bailly M, Vialard F, Selva J (2013) Small human sperm vacuoles observed under high magnification are pocket-like nuclear concavities linked to chromatin condensation failure. Reprod Biomed Online 27:201–211PubMedCrossRefGoogle Scholar
  11. Bungum M, Bungum L, Humaidan P (2006) A prospective study, using sibling oocytes, examining the effect of 30 seconds versus 90 minutes gamete co-incubation in IVF. Hum Reprod 21:518–523PubMedCrossRefGoogle Scholar
  12. Casper RF, Meriano JS, Jarvi KA, Cowan L, Lucato ML (1996) The hypo-osmotic swelling test for selection of viable sperm for intracytoplasmic sperm injection in men with complete asthenozoospermia. Fertil Steril 65:972–976PubMedCrossRefGoogle Scholar
  13. Cohen J, Malter H, Fehilly C, Wright G, Elsner C, Kort H, Massey J (1988) Implantation of embryos after partial opening of oocyte zona pellucida to facilitate sperm penetration. Lancet 2:162PubMedCrossRefGoogle Scholar
  14. Daya S, Kohut J, Gunby J, Younglai E (1990) Influence of blood clots in the cumulus complex on oocyte fertilization and cleavage. Hum Reprod 5:744–746PubMedCrossRefGoogle Scholar
  15. Dozortsev D, Nagy P, Abdelmassih S, Oliveira F, Brasil A, Abdelmassih V, Diamond M, Abdelmassih R (2004) The optimal time for intracytoplasmic sperm injection in the human is from 37 to 41 hours after administration of human chorionic gonadotropin. Fertil Steril 82:1492–1496PubMedCrossRefGoogle Scholar
  16. Ebner T, Yaman C, Moser M, Sommergruber M, Hartl J, Tews G (2001) Laser assisted immobilization of spermatozoa prior to intracytoplasmic sperm injection in humans. Hum Reprod 16:2628–2631PubMedCrossRefGoogle Scholar
  17. Ebner T, Moser M, Yaman C, Sommergruber M, Tews G (2002) Successful birth after laser assisted immobilization of spermatozoa before intracytoplasmic injection. Fertil Steril 78:417–418PubMedCrossRefGoogle Scholar
  18. Ebner T, Moser M, Sommergruber M, Jesacher K, Tews G (2004) Complete oocyte activation failure after ICSI can be overcome by a modified injection technique. Hum Reprod 19:1837–1841PubMedCrossRefGoogle Scholar
  19. Ebner T, Moser M, Sommergruber M, Shebl O, Tews G (2006) Incomplete denudation of oocytes prior to ICSI enhances embryo quality and blastocyst development. Hum Reprod 21:2972–2977PubMedCrossRefGoogle Scholar
  20. Ebner T, Moser M, Shebl O, Sommergruber M, Yaman C, Tews G (2008) Blood clots in the cumulus-oocyte complex predict poor oocyte quality and post-fertilization development. Reprod Biomed Online 16:801–807PubMedCrossRefGoogle Scholar
  21. Ebner T, Shebl O, Moser M, Mayer RB, Arzt W, Tews G (2011a) Easy sperm processing technique allowing exclusive accumulation and later usage of DNA-strandbreak-free spermatozoa. Reprod Biomed Online 22:37–43PubMedCrossRefGoogle Scholar
  22. Ebner T, Moser M, Shebl O, Mayer R, Tews G (2011b) Assisting in vitro fertilization by manipulating cumulus-oocyte-complexes either mechanically or enzymatically does not prevent IVF failure. J Turk Ger Gynecol Assoc 12:135–139PubMedPubMedCentralCrossRefGoogle Scholar
  23. Ebner T, Filicori M, Tews G, Parmegiani L (2011c) A plea for a more physiological ICSI. Andrologia 44(1):2–19PubMedGoogle Scholar
  24. Ebner T, Tews G, Mayer RB, Ziehr S, Arzt W, Costamoling W, Shebl O (2011d) Pharmacological stimulation of sperm motility in frozen and thawed testicular sperm using the dimethylxanthine theophylline. Fertil Steril 96:1331–1336PubMedCrossRefGoogle Scholar
  25. Ebner T, Montag M, Oocyte Activation Study Group (2015) Live birth after artificial oocyte activation using a ready-to-use ionophore: a prospective multicentre study. Reprod Biomed Online 30:359–365PubMedCrossRefGoogle Scholar
  26. ESHRE Special Interest Group Embryology und Alpha Scientists in Reproductive Medicine (2017) The Vienna consensus: report of an expert meeting on the development of ART laboratory performance indicators. Reprod Biomed Online.  https://doi.org/10.1016/j.rbmo.2017.06.015
  27. Evison M, Pretty C, Taylor E, Franklin C (2009) Human recombinant hyaluronidase (Cumulase) improves intracytoplasmic sperm injection survival and fertilization rates. Reprod Biomed Online 18:811–814PubMedCrossRefGoogle Scholar
  28. Gianaroli L, Fiorentino A, Magli MC, Ferraretti AP, Montanaro N (1996) Prolonged sperm-oocyte exposure and high sperm concentration affect human embryo viability and pregnancy rate. Hum Reprod 11:2505–2511CrossRefGoogle Scholar
  29. Greco E, Scarselli F, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Franco G, Anniballo N, Mendoza C, Tesarik J (2005) Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular spermatozoa. Hum Reprod 20:226–230PubMedCrossRefGoogle Scholar
  30. Hardarson T, Lundin K, Hamberger L (2000) The position of the metaphase II spindle cannot be predicted by the location of the first polar body in the human oocyte. Hum Reprod 15:1372–1376PubMedCrossRefGoogle Scholar
  31. Huszar G, Jak A, Sakkas OCC, Cayli S, Delpiano E, Ozkavukcu S (2007) Fertility testing and ICSI sperm selection by hyaluronic acid binding: clinical and genetics aspects. Reprod Biomed Online 14:650–663PubMedCrossRefGoogle Scholar
  32. Hwang JL, Seow KM, Lin YH, Hsieh BC, Huang LW, Chen HJ, Huang SC, Chen CY, Chen PH, Tzeng CR (2005) IVF versus ICSI in sibling oocytes from patients with polycystic ovarian syndrome: a randomized controlled trial. Hum Reprod 20:1261–1265PubMedCrossRefGoogle Scholar
  33. Kastrop PM, Weima SM, Van Kooij RJ, Te Velde ER (1999) Comparison between intracytoplasmic sperm injection and in-vitro fertilization (IVF) with high insemination concentration after total fertilization failure in a previous IVF attempt. Hum Reprod 14:61–69CrossRefGoogle Scholar
  34. Kattera S, Chen C (2003) Short coincubation of gametes in in vitro fertilisation improves implantation and pregnancy rates: a prospective, randomised, controlled study. Fertil Steril 80:1017–1021PubMedCrossRefGoogle Scholar
  35. Kim JW, Kim SD, Yang SH, Yoon SH, Jung JH, Lim JH (2014) Successful pregnancy after SrCl2 oocyte activation in couples with repeated low fertilization rates following calcium ionophore treatment. Syst Biol Reprod Med 60:177–182PubMedCrossRefGoogle Scholar
  36. Komsky-Elbaz A, Raziel A, Friedler S, Strassburger D, Kasterstein E, Komarovsky D, Ron-El R, Ben-Ami I (2013) Conventional IVF versus ICSI in sibling oocytes from couples with endometriosis and normozoospermic semen. J Assist Reprod Genetics 30:251–257CrossRefGoogle Scholar
  37. Krause I, Pohler U, Grosse S, Shebl O, Petek E, Chandra A, Ebner T (2016) Characterization of the injection funnel during intracytoplasmic sperm injection reflects cytoplasmic maturity of the oocyte. Fertil Steril 106:1101–1106PubMedCrossRefGoogle Scholar
  38. Liu J, Tsai YL, Katz E, Compton G, Garcia JE, Baramki TA (1997) High fertilization rate obtained after intracytoplasmic sperm injection with 100 % nonmotile spermatozoa selected by using a simple modified hypo-osmotic swelling test. Fertil Steril 68:373–375PubMedCrossRefGoogle Scholar
  39. Lundqvist M, Johansson U, Lundquist Ø, Milton K, Westin C, Simberg N (2001) Reducing the time of coincubation of gametes in human in vitro fertilisation has no beneficial effects. Reprod Biomed Online 3:21–24PubMedCrossRefGoogle Scholar
  40. Montag M, Rink K, Delacrétaz G, van der Ven H (2000) Laser-induced immobilization and plasma membrane permeabilization in human spermatozoa. Hum Reprod 15:846–852PubMedCrossRefGoogle Scholar
  41. Montag M, van der Ven H, German Pronuclear Morphology Study Group (2001) Evaluation of pronuclear morphology as the only selection criterion for further embryo culture and transfer: results of a prospective multicentre study. Hum Reprod 16:2384–2389PubMedCrossRefGoogle Scholar
  42. Montag M, Köster M, van der Ven K, Bohlen U, van der Ven H (2012) The benefit of artificial oocyte activation is dependent on the fertilization rate in a previous treatment cycle. Reprod Biomed Online 24:521–526PubMedCrossRefGoogle Scholar
  43. Moomjy M, Scott Sills E, Rosenwaks Z, Palermo GD (1998) Implications of complete fertilization failure after intracytoplasmic sperm injection for subsequent fertilization and reproductive outcome. Hum Reprod 13:2212–2216PubMedCrossRefGoogle Scholar
  44. Moser M, Ebner T, Sommergruber M, Gaisswinkler U, Jesacher K, Puchner M, Wiesinger R, Tews G (2004) Laser-assisted zona pellucida thinning prior to routine ICSI. Hum Reprod 19:573–578PubMedCrossRefGoogle Scholar
  45. Moura BR, Gurgel MC, Machado SP, Marques PA, Rolim JR, Lima MC, Salgueiro LL (2017) Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality. JBRA Asist Reprod 21:27–30Google Scholar
  46. Nagy ZP, Liu J, Joris H, Desmet B, Van Ranst H, Vankelecom A, Devroey P, Van Steirteghem AC (1995) The influence of the site of sperm deposition and mode of oolemma breakage at intracytoplasmic sperm injection on fertilization and embryo development rates. Hum Reprod 10:3171–3177PubMedCrossRefGoogle Scholar
  47. Nagy ZP, Oliveira SA, Abdelmassih V, Abdelmassih R (2002) Novel use of laser to assist ICSI for patients with fragile oocytes: a case report. Reprod Biomed Online 4:27–31PubMedCrossRefGoogle Scholar
  48. Ng SC, Bongso A, Ratnam SS, Sathananthan H, Chan CL, Wong PC, Hagglund L, Anandakumar C, Wong YC, Goh VH (1988) Pregnancy after transfer of sperm under zona. Lancet 2:790CrossRefGoogle Scholar
  49. Nikiforaki D, Vanden Meerschaut F, de Roo C, Lu Y, Ferrer-Buitrago M, de Sutter P, Heindryckx B (2016) Effect of two assisted oocyte activation protocols used to overcome fertilization failure on the activation potential and calcium releasing pattern. Fertil Steril 105:798–806PubMedCrossRefGoogle Scholar
  50. Oliveira NM de, Sanchez R, Fiesta S, Lopez Salgado T, Rodríguez R, Bethencourt JC, Blanes Zamora R (2004) Pregnancy with frozen–thawed and fresh testicular biopsy after motile and immotile sperm microinjection, using the mechanical touch technique to assess viability. Hum Reprod 19: 262–265Google Scholar
  51. Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, Evenson D, AJJr T, Alvarez JG (2001) Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod 16:1912–1921PubMedCrossRefGoogle Scholar
  52. Palermo G, Joris H, Devroey P, Van Steirteghem AC (1992) Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 340:17–18CrossRefGoogle Scholar
  53. Parinaud J, Vieitez G, Milhet P, Richoilley G (1998) Use of a plant enzyme preparation (Coronase) instead of hyaluronidase for cumulus cell removal before intracytoplasmic sperm injection. Hum Reprod 13:1933–1935PubMedCrossRefGoogle Scholar
  54. Parmegiani L, Cognigni GE, Ciampaglia W, Pocognoli P, Marchi F, Filicori M (2010a) Efficiency of hyaluronic acid (HA) sperm selection. J Assist Reprod Genet 27:13–16PubMedCrossRefGoogle Scholar
  55. Parmegiani L, Cognigni GE, Bernardi S, Troilo E, Ciampaglia W, Filicori M (2010b) „Physiologic ICSI“: hyaluronic acid (HA) favors selection of spermatozoa without DNA fragmentation and with normal nucleus, resulting in improvement of embryo quality. Fertil Steril 93:598–604PubMedCrossRefGoogle Scholar
  56. Patrizio P, Silber S, Ord T, Balmaceda JP, Asch RH (1988) Two births after microsurgical sperm aspiration in congenital absence of vas deferens. Lancet 2:1364PubMedCrossRefGoogle Scholar
  57. Plachot M, Belaisch-Allart J, Mayenga JM, Chouraqui A, Tesquier L, Serkine AM (2002) Outcome of conventional IVF and ICSI on sibling oocytes in mild male factor infertility. Hum Reprod 17:362–369PubMedCrossRefGoogle Scholar
  58. Primi MP, Senn A, Montag M, Van der Ven H, Mandelbaum J, Veiga A, Barri P, Germond M (2004) A European multicentre prospective randomized study to assess the use of assisted hatching with a diode laser and the benefit of an immunosuppressive/antibiotic treatment in different patient populations. Hum Reprod 19:2325–2333PubMedCrossRefGoogle Scholar
  59. Rattanachaiyanont M, Leader A, Léveillé MC (1999) Lack of correlation between oocyte-corona-cumulus complex morphology and nuclear maturity of oocytes collected in stimulated cycles for intracytoplasmic sperm injection. Fertil Steril 71:937–940PubMedCrossRefGoogle Scholar
  60. Rienzi L, Greco E, Ubaldi F, Iacobelli M, Martinez F, Tesarik J (2001) Laser-assisted intracytoplasmic sperm injection. Fertil Steril 76:1045–1047PubMedCrossRefGoogle Scholar
  61. Rybouchkin AV, Van der Straeten F, Quatacker J, De Sutter P, Dhont M (1997) Fertilization and pregnancy after assisted oocyte activation and intracytoplasmic sperm injection in a case of round-headed sperm associated with deficient oocyte activation capacity. Fertil Steril 68:1144–1147PubMedCrossRefGoogle Scholar
  62. Seiringer M, Maurer M, Shebl O, Dreier K, Tews G, Ziehr S, Schappacher-Tilp G, Petek E, Ebner T (2013) Efficacy of a sperm-selection chamber in terms of morphology, aneuploidy and DNA packaging. Reprod Biomed Online 27:81–88PubMedCrossRefGoogle Scholar
  63. Silber SJ, Nagy ZP, Liu J, Godoy H, Devroey P, Van Steirteghem AC (1994) Conventional in-vitro fertilization versus intracytoplasmic sperm injection for patients requiring microsurgical sperm aspiration. Hum Reprod 9:1705–1709PubMedCrossRefGoogle Scholar
  64. Stanger JD, Stevenson K, Lakmaker A, Woolcott R (2001) Pregnancy following fertilization of zona free, coronal cell intact human ova. Hum Reprod 16:164–167PubMedCrossRefGoogle Scholar
  65. Steptoe PC, Edwards RG (1978) Birth after the reimplantation of a human embryo. Lancet 2:366CrossRefGoogle Scholar
  66. Swann K, Windsor S, Campbell K, Elgmati K, Nomikos M, Zernicka-Goetz M, Amso N, Lai FA, Thomas A, Graham C (2012) Phospholipase C-ζ-induced Ca2+ oscillations cause coincident cytoplasmic movements in human oocytes that failed to fertilize after intracytoplasmic sperm injection. Fertil Steril 97:742–747PubMedPubMedCentralCrossRefGoogle Scholar
  67. Tesarik J, Rienzi L, Ubaldi F, Mendoza C, Greco E (2002) Use of a modified intracytoplasmic sperm injection technique to overcome sperm-borne and oocyte-borne oocyte activation failures. Fertil Steril 78:619–624PubMedCrossRefGoogle Scholar
  68. Tomsu M, Sharma V, Miller D (2002) Embryo quality and IVF treatment outcomes may correlate with different sperm comet assay parameters. Hum Reprod 17:1856–1862PubMedCrossRefGoogle Scholar
  69. Van de Velde H, Nagy ZP, Joris H, De Vos A, Van Steirteghem AC (1997) Effects of different hyaluronidase concentrations and mechanical procedures for cumulus cell removal on the outcome of intracytoplasmic sperm injection. Hum Reprod 12:2246–2250PubMedCrossRefGoogle Scholar
  70. Van de Velde H, De Vos A, Joris H, Nagy ZP, Van Steirteghem AC (1998) Effect of timing of oocyte denudation and micro-injection on survival, fertilization and embryo quality after intracytoplasmic sperm injection. Hum Reprod 13:3160–3164PubMedCrossRefGoogle Scholar
  71. Van den Bergh M, Bertrand E, Biramane J, Englert Y (1995) Importance of breaking a spermatozoon’s tail before intracytoplasmic injection: a prospective randomized trial. Hum Reprod 10:2819–2820PubMedCrossRefGoogle Scholar
  72. Van den Bergh MJ, Fahy-Deshe M, Hohl MK (2009) Pronuclear zygote score following intracytoplasmic injection of hyaluronan-bound spermatozoa: a prospective randomized study. Reprod Biomed Online 19:796–801CrossRefGoogle Scholar
  73. Vanderzwalmen P, Bertin G, Lejeune B, Nijs M, Vandamme B, Schoysman R (1996) Two essential steps for a successful intracytoplasmic injection: Injection of immobilized spermatozoa after rupture of the oolemma. Hum Reprod 11:540–547PubMedCrossRefGoogle Scholar
  74. Veeck LL (1999) The human oocyte. In: Veeck LL (Hrsg) An atlas of human gametes and conceptuses, 1. Aufl. Parthenon Publishing, New York/LondonCrossRefGoogle Scholar
  75. Wöber M, Ebner T, Steiner SL, Strohmer H, Oppelt P, Plas E, Obruca A (2015) A new method to process testicular sperm: combining enzymatic digestion, accumulation of spermatozoa, and stimulation of motility. Arch Gynecol Obstet 291:689–694PubMedCrossRefGoogle Scholar
  76. World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen, 5. Aufl. WHO Press, GenevaGoogle Scholar
  77. Yanagida K, Katayose H, Yazawa H, Kimura Y, Konnai K, Sato A (1999) The usefulness of a piezo-micromanipulator in intracytoplasmic sperm injection in humans. Hum Reprod 14:448–453PubMedCrossRefGoogle Scholar
  78. Yoon SY, Eum JH, Lee JE, Lee HC, Kim YS, Han JE, Won HJ, Park SH, Shim SH, Lee WS, Fissore RA, Lee DR, Yoon TK (2012) Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes. Hum Reprod 27:1768–1780PubMedCrossRefGoogle Scholar

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© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2020

Authors and Affiliations

  1. 1.Klinik für Gynäkologie, Geburtshilfe und gyn. EndokrinologieKepler Universitätsklinikum GmbHLinzÖsterreich
  2. 2.Facharzt-Zentrum für Kinderwunsch, Pränatale Medizin, Endokrinologie und Osteologie Hamburg GmbHamedes expertsHamburgDeutschland
  3. 3.Universitäts-Frauenklinik LübeckLübeckDeutschland

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