Immunological methods

  • Peter B. Luppa
  • Ralf Junker
  • Ingolf Schimke
  • Enno Stürenburg


The specificity of antibodies for their molecular antigen counterparts is the basis for immunoassay technology, which is also employed in POCT devices, enabling the synthesis of qualitative and quantitative results. Immunological POCT systems generally rely on electrochemical (potentiometric, amperometric, conductometric, capacitive) or optic sensors for detection.


  1. 1.
    Agarwal R, Heinz T (2001) Bedside hemoglobinometry in hemodialysis patients: lessons from point-of-care testing. ASAIO J 47: 240–243CrossRefPubMedGoogle Scholar
  2. 2.
    Briggs C, Guthrie D, Hyde K, Mackie I, Parker N, Popek M et al. British Committee for Standards in Haematology (2008) Guidelines for point-of-care testing: haematology. Br J Haematol 142:904–915CrossRefPubMedGoogle Scholar
  3. 3.
    Bruno JG (2015) Predicting the uncertain future of aptamer-based diagnostics and therapeutics. Molecules 20:6866–6887CrossRefPubMedGoogle Scholar
  4. 4.
    Collinson P (2015) Detecting cardiac events – state-of-the-art. Ann Clin Biochem 52:702–704CrossRefPubMedGoogle Scholar
  5. 5.
    Dunn SG, Visnich MR (2002) Home-based point-of-care testing. In: Kost GJ (ed) Principles and practice of point-of-care testing. Lippincott Williams & Wilkins, Philadelphia, pp 376–390Google Scholar
  6. 6.
    Ekins RP (1999) Immunoassay and other ligand assays: from isotopes to luminescence. J Clin Ligand Assay 22: 61–77Google Scholar
  7. 7.
    Friedewald S, Finke EJ, Dobler G (2006) Patientennahe Diagnostik in Krisensituationen. J Lab Med 30: 211–221Google Scholar
  8. 8.
    Hafner G, Peetz D, Dati F (2003) Patientennahe Bestimmung der Troponine zur Diagnostik akuter Koronarsyndrome. J Lab Med 27: 279–287Google Scholar
  9. 9.
    Inverness medical (2006) BioStar OIA GC. An enhanced optical immunoassay for the rapid detection of Neisseria gonorrhoeae from female endocervical swabs and male urine specimens. Product insert, Revision 02. Inverness Medical Innovations, Waltham, MA, USAGoogle Scholar
  10. 10.
    Khati M (2010) The future of aptamers in medicine. J Clin Pathol 63:480–487CrossRefPubMedGoogle Scholar
  11. 11.
    Klewitz TM (2005) Entwicklung eines quantitativen Lateral-Flow-Immunoassays zum Nachweis von Analyten in geringsten Konzentrationen. Doctoral dissertation, Hannover UniversityGoogle Scholar
  12. 12.
    Kost GJ, Ferguson WJ, Hoe J, Truong AT, Banpavichit A, Kongpila S (2015) The Ebola Spatial Care Path™: Accelerating point-of-care diagnosis, decision making, and community resilience in outbreaks. Am J Disaster Med 10:121–143Google Scholar
  13. 13.
    Lehman CA, Giacini JM (2010) Point-of-care testing in the home and community environment: key ingredients for tomorrow´s community health. In: Price CP, St John A, Kricka LJ (eds) Point-of care testing, 3rd edition. AACC Press, Washington, pp 311–321Google Scholar
  14. 14.
    Luppa PB, Müller C, Schlichtiger A, Schlebusch H (2011) Point-of-care testing (POCT): Current techniques and future perspectives. Tr Anal Chem 30:887–898CrossRefGoogle Scholar
  15. 15.
    Luppa PB, Bietenbeck A, Beaudoin C, Giannetti A (2016) Clinically relevant analytical techniques, organizational concepts for application and future perspectives of point-of-care testing. Biotechnol Adv. 2016 Jan 22. pii: S0734–9750(16)30004–0. doi: 10.1016/j.biotechadv.2016.01.003CrossRefPubMedPubMedCentralGoogle Scholar
  16. 16.
    Mairal T, Ozalp VC, Lozano Sánchez P, Mir M, Katakis I, O’Sullivan CK (2008) Aptamers: molecular tools for analytical applications.Anal Bioanal Chem 390:989–1007CrossRefPubMedGoogle Scholar
  17. 17.
    Peetz D, Hafner G, Lackner KJ (2005) Patientennahe Bestimmung natriuretischer Peptide. J Lab Med 29: 219–228Google Scholar
  18. 18.
    Reinert RR (2007) Streptokokken-Schnelltests. J Lab Med 31: 280–293Google Scholar
  19. 19.
    Robinson JE, Wakelin M, Ellis JE (2007) Increased pregnancy rate with use of the Clearblue Easy Fertility Monitor. Fertil Steril 87:329–334Google Scholar
  20. 20.
    Schweiger B (2006) Influenza rapid tests – advantages and limitations. J Lab Med 30: 219–215Google Scholar
  21. 21.
    Siebenhofer A, Jeitler K, Horvath K, Habacher W, Schmidt L, Semlitsch T (2014) Self-management of oral anticoagulation. Dtsch Ärztebl Int 111:83–91Google Scholar
  22. 22.
    Sokoll LJ, Wians FH Jr, Remaley AT (2004) Rapid intraoperative immunoassay of parathyroid hormone and other hormones: a new paradigm for point-of-care testing. Clin Chem 50: 1126–1135CrossRefPubMedGoogle Scholar
  23. 23.
    Takahashi H, Otsuka Y, Patterson BK (2010) Diagnostic tests for influenza and other respiratory viruses: determining performance specifications based on clinical setting. J Infect Chemother 16:155–161CrossRefPubMedGoogle Scholar
  24. 24.
    Tang Z, Louie RF, Kost GJ (2002) Principles and performance of point-of-care testing instruments. In: Kost GJ (ed) Principles and practice of point-of-care Testing. Lippincott Williams & Wilkins, Philadelphia, pp 67–92Google Scholar
  25. 25.
    Thomas L (2012) Labor und Diagnose, 8th Edition, TH Books, Frankfurt/MainGoogle Scholar
  26. 26.
    Wolfbeis OS (2000) Fiber-optic chemical sensors and biosensors. Anal Chem 72: 81R–89RCrossRefGoogle Scholar
  27. 27.
    Wood F, Brookes-Howell L, Hood K, Cooper L, Verheij T, Goossens H, Little P, Godycki-Cwirko M, Adriaenssens N, Jakobsen K, Butler CC (2011) A multi-country qualitative study of clinicians’ and patients’ views on point of care tests for lower respiratory tract infection. Fam Pract 28:661–669CrossRefPubMedGoogle Scholar
  28. 28.
    Yonzon CR, Zhang X, Zhao J, van Duyne RP (2007) Surface-Enhanced Nanosensors. Spectroscopy 22:42–56Google Scholar
  29. 29.
    Zhou W, Huang PJ, Ding J, Liu J (2014) Aptamer-based biosensors for biomedical diagnostics. Analyst 139:2627–2640CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Peter B. Luppa
    • 1
  • Ralf Junker
    • 2
  • Ingolf Schimke
    • 3
  • Enno Stürenburg
    • 4
  1. 1.Klinikum rechts der TU MünchenInstitut für Klinische Chemie und PathobiochemieMünchenGermany
  2. 2.Universitätsklinikum Schleswig-HolsteinInstitut für Klinische ChemieKielGermany
  3. 3.Medizinische Klinik (Kardiologie), Charité-Universitätsmedizin BerlinBerlinGermany
  4. 4.Dr. Staber & KollegenHamburgGermany

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