Molecular biological tests

  • Enno Stürenburg
  • Norbert Gässler
  • Aline Schröder
  • Udo Reischl


Molecular biological testing has become a mainstay in the repertoire of infectious disease diagnostics like in no other field of medicine. Direct pathogen detection primarily relies upon two main methods: the polymerase chain reaction (PCR) and immunochromatography – often supplied as test strips or test cards. Alongside pathogen detection, the PCR method has the advantage that it can simultaneously analyze resistance determinants and/or virulence factors. In the age of multidrug-resistant (nosocomial) pathogens, rapid and reliable molecular biological differentiation is becoming increasingly important given the urgency indicated to effectively isolate affected patients at the earliest possible chance.


  1. 1.
    St John A, Price CP (2014) Existing and emerging technologies for point-of-care testing. Clin Biochem Rev 35:155–167Google Scholar
  2. 2.
    Niemz A, Ferguson TM, Boyle DS (2011) Point-of-care nucleic testing for infectious diseases. Trends Biotechnol 240–250Google Scholar
  3. 3.
    Craw P, Balachandran W (2012) Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review. Lab Chip 12:2469–2486CrossRefPubMedGoogle Scholar
  4. 4.
    Stürenburg E, Junker R (2009) Patientennahe Diagnostik in der Mikrobiologie. Dtsch Ärzteblatt 106:48–54Google Scholar
  5. 5.
    Reischl U, Drosten C, Geißdörfer W, Göbel U, Hoffmann KS, Mauch H, Meyer T, Moter A, von Müller L, Panning M et al. (2011) MiQ 1: Nukleinsäure-Amplifikationstechniken (NAT), 3rd Edition, In: Mikrobiologisch-infektiologische Qualitätsstandards (MiQ) Qualitätsstandards in der mikrobiologisch-infektiologischen Diagnostik. Urban & Fischer, Munich, pp 1–80Google Scholar
  6. 6.
    Nie S, Roth RB, Stiles J, Mikhlina A, Lu X, Tang YW, Babady NE (2014) Evaluation of Alere i Influenza A and B for rapid detection of influenza viruses A and B. J Clin Microbiol 52(9):3339–3344Google Scholar
  7. 7.
    Cohen DM, Russo ME, Jaggi P, Kline J, Gluckman W, Parekh A (2015) Multicenter clinical evaluation of the novel Alere i Strep A isothermal nucleic acid amplification Test. J Clin Microbiol 53(7):2258–2261CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Scott L, Gous N, Carmona S, Stevens W (2015) Laboratory Evaluation of the Liat HIV Quant (IQuum) whole-blood and plasma HIV-1 viral load assays for Point-of-Care Testing in South Africa. J Clin Microbiol 53(5): 1616–1621CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Chen L, Tian Y, Chen S, Liesenfeld O (2015) Performance of the Cobas Influenza A/B Assay for rapid PCR-based detection of influenza compared to Prodesse ProFlu+ and viral culture. Eur J Microbiol Immunol (Bp) 5(4): 236–245CrossRefPubMedGoogle Scholar
  10. 10.
    Pearce DM, Shenton DP, Holden J, Gaydos CA (2011) Evaluation of a novel electrochemical detection method for Chlamydia trachomatis: Application for Point-of-Care diagnostics. IEEE Trans Biomed Eng 58(3): 755–758CrossRefPubMedGoogle Scholar
  11. 11.
    Pearce DM, Styles DN, Hardick JP, Gaydos CA (2013) A new rapid molecular point-of-care assay for Trichomonas vaginalis: preliminary performance data. Sex Transm Infect 89(6): 495–497CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Goldenberg SD, Bisnauthsing KN, Patel A, Postulka A, Wyncoll D, Schiff R, French GL (2014) Point-of-Care Testing for Clostridium Difficile infection: A real-world feasibility study of a rapid molecular test in two hospital settings. Infect Dis Ther 3(2):295–306CrossRefPubMedPubMedCentralGoogle Scholar
  13. 13.
    Parcell BJ, Phillips G (2014) Use of Xpert MRSA PCR point-of-care testing beyond the laboratory. J Hosp Infect 87(2):119–121CrossRefPubMedGoogle Scholar
  14. 14.
    Stimpfle F, Karathanos A, Droppa M, Metzger J, Rath D, Müller K, Tavlaki E, Schäffeler E, Winter S, Schwab M, Gawaz M, Geisler T (2014) Impact of point-of-care testing for CYP2C19 on platelet inhibition in patients with acute coronary syndrome and early dual antiplatelet therapy in the emergency setting. Thromb Res 134(1):105–110CrossRefPubMedGoogle Scholar
  15. 15.
    Spina A, Kerr KG, Cormican M, Barbut F, Eigentler A, Zerva L, Tassios P, Popescu GA, Rafila A, Eerola E, Batista J, Maass M, Aschbacher R, Olsen KE, Allerberger F (2015) Spectrum of enteropathogens detected by the Film-Array GI Panel in a multicentre study of community-acquired gastroenteritis. Clin Microbiol Infect 21(8):719–728CrossRefPubMedGoogle Scholar
  16. 16.
    Babady NE (2013) The FilmArray respiratory panel: an automated, broadly multiplexed molecular test for the rapid and accurate detection of respiratory pathogens. Expert Rev Mol Diagn 13(8):779–788CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Enno Stürenburg
    • 1
  • Norbert Gässler
    • 2
  • Aline Schröder
    • 3
  • Udo Reischl
    • 4
  1. 1.Dr. Staber & KollegenHamburgGermany
  2. 2.Zentrum für Labordiagnostik, St. Bernward-KrankenhausHildesheimGermany
  3. 3.Med-Lab Medizinische Dienstleistungs GmbHHamburgGermany
  4. 4.Universitätsklinikum RegensburgInstitut für Medizinische Mikrobiologie und HygieneRegensburgGermany

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