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Adenosine triphosphatase activity of mitochondria

  • Masahiro Chiga
Chapter
Part of the Handbuch der Physiologisch- und Pathologisch-Chemischen Analyse book series (HOPPE-SEYLER)

Zusammenfassung

It was shown a number of years ago that rat liver mitochondria contained an activity which can liberate inorganic phosphate from ATP1. Since then, numerous studies dealing with this mitochondrial ATPase activity have established that its action is different from that of other phosphatases. The extensive study of the mitochondrial ATPase activity has been motivated by the assumption that mitochondrial ATPase may be a part of the enzyme system involved in oxidative phosphorylation2–5.

Abbreviations

ATP

adenosine triphosphate

ATPase

adenosine triphosphatase

ADP

adenosine diphosphate

Pi

orthophosphate

DNP

2,4-dinitrophenol

Mw

mitochondria

EDTA

ethylenediaminetetraacetate

ITP

inosine triphosphate

GTP

guanosine triphosphate

UTP

uridine triphosphate

CTP

cytidine triphosphate

AMP

adenosine 5’-monophosphate

Tris

trishydroxymethyl-aminomethane

DPN and DPNH

diphosphopyridine nucleotide, oxidized form and reduced form

FAD

flavin adenine dinucleotide

FMN

flavin mononucleotide

DOC

deoxycholate

pCMB

p-chloro-mercuribenzoate

DCPIP

2,6-dichlorophenolindophenol.

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Literatur

  1. 1.
    Schneider, W. C: J. biol. Ch. 165, 585 (1946).Google Scholar
  2. 2.
    Hunter, F. E. jr.; in: McElroy-Glass, Phosphorus Metabolism. Vol. 1, p. 297.Google Scholar
  3. 3.
    Kielley, W. W., and K. K. Kielley: J. biol. Ch. 191, 485 (1951).Google Scholar
  4. 4.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  5. 5.
    Potter, V. K., P. Siekevitz and H. C. Simonson: J. biol. Ch. 205, 893 (1953).Google Scholar
  6. 6.
    Drysdale, G. R., and M. Cohn: J. biol. Ch. 233, 1574 (1958).Google Scholar
  7. 1.
    Kielley, W. W., and E. K. Kielley: J. biol. Cli. 191, 485 (1951).Google Scholar
  8. 2.
    Lardy, H. A., and IT. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  9. 3.
    Chappell, J. B., and S. V. Perry: Biochem. J. 55, 586 (1953).PubMedGoogle Scholar
  10. 4.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Eacker: J. biol. Cli. 235, 3322 (1960).Google Scholar
  11. 5.
    Von Korff, E. W.: Science, N.Y. 126, 308 (1957).Google Scholar
  12. 6.
    Blecher, M., and A. White: J. biol. Ch. 235, 3404 (1960).Google Scholar
  13. 7.
    Dorner, M., and E. Eeich: Biochim. biophys. Acta 48, 534 (1961).PubMedGoogle Scholar
  14. 8.
    Braganca, B. M., and I. Aravindakshan: Biochem. biophys. Ees. Comm. 3, 484 (1960).Google Scholar
  15. 9.
    Cooper, C, and A. L. Lehninger: J. biol. Ch. 224, 547 (1957).Google Scholar
  16. 10.
    Cooper, C: J. biol. Ch. 235, 1815 (1960).Google Scholar
  17. 11.
    Wadkins, C. L.: J. biol. Ch. 235, 3300 (1960).Google Scholar
  18. 12.
    Bronk, J. E., and W. W. Kielley: Biochim. biophys. Acta 24, 440 (1957).PubMedGoogle Scholar
  19. 13.
    Kielley, W. W., and E. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  20. 1.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  21. 2.
    Potter, V. E., P. Siekevitz and H. C. Simonson: J. biol. Ch. 205, 893 (1953).Google Scholar
  22. 3.
    Kielley, W. W., and E. K. Kielley: J. biol. Ch. 191, 485 (1951).Google Scholar
  23. 4.
    Siekevitz, P., H. Low, L. Ernster and O. Lindberg: Biochim. biophys. Acta 29, 378 (1958).PubMedGoogle Scholar
  24. 5.
    Beyer, E. E.: Biochim. biophys. Acta 32, 588 (1959).PubMedGoogle Scholar
  25. 6.
    Cooper, C, and A. L. Lehninger: J. biol. Ch. 224, 547 (1957).Google Scholar
  26. 7.
    Beyer, E. E., S. L. Lamberg and M. A. Neyman: Canad. J. Biochem. Physiol. 39, 73 (1961)Google Scholar
  27. 8.
    Cooper, C: Biochim. biophys. Acta 30, 484 (1958).PubMedGoogle Scholar
  28. 9.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Eacker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  29. 10.
    Beyer, E. E.: Biochim. biophys. Acta 41, 552 (1960).PubMedGoogle Scholar
  30. 11.
    Cooper, C: J. biol. Ch. 235, 1815 (1960).Google Scholar
  31. 1.
    Myers, D. K., and E. C. Slater: Biochem. J. 67, 558 (1957).PubMedGoogle Scholar
  32. 2.
    Penniall, K.: Biochim. biophys. Acta 44, 395 (1960).PubMedGoogle Scholar
  33. 3.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  34. 4.
    Cooper, C: Biochim. biophys. Acta 30, 484 (1958).PubMedGoogle Scholar
  35. 5.
    Hemker, H. C, and W. C. Hülsmann: Biochim. biophys. Acta 48, 221 (1961).PubMedGoogle Scholar
  36. 6.
    Potter, V. R., P. Siekevitz and H. C. Simonson: J. biol. Ch. 205, 893 (1953).Google Scholar
  37. 7.
    Braganca, B. M., and I. Aravindakshan: Biochem. biophys. Res. Comm. 3, 484 (1960).Google Scholar
  38. 8.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Racker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  39. 9.
    Chappell, J. B., and S. V. Perry: Biochem. J. 55, 586 (1953).PubMedGoogle Scholar
  40. 10.
    Beyer, R. E.: Biochim. biophys. Acta 41, 552 (1960).PubMedGoogle Scholar
  41. 11.
    Beyer, R. E.: Exp. Cell Res. 21, 217 (1960).Google Scholar
  42. 12.
    Kielley, W. W., and R. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  43. 1.
    Kielley, W. W., and E. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  44. 2.
    Cooper, C.: Biochim. biophys. Acta 30, 484 (1958).PubMedGoogle Scholar
  45. 3.
    Cooper, C, and A. L. Lehninger: J. biol. Ch. 224, 547 (1957).Google Scholar
  46. 4.
    Gamble Jr., J. L., and A. L. Lehninger: J. biol. Ch. 223, 921 (1956).Google Scholar
  47. 5.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Racker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  48. 6.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  49. 7.
    Berg, P., and W. K. Joklik: J. biol. Ch. 210, 657 (1954).Google Scholar
  50. 8.
    Chiga, M., and G. W. E. Plaut: J. biol. Ch. 234, 3059 (1959).Google Scholar
  51. 9.
    Chappell, J. B., and S. V. Perry: Biochem. J. 55, 586 (1953).PubMedGoogle Scholar
  52. 1.
    Novikoff, A. B.: 3. Int. Congr. B.ochem. Bruxelles 1956, p. 315.Google Scholar
  53. 2.
    Gregg, C. T., C. R. Heisler and L. F. Remmert: Biochim. biophys. Acta 31, 593 (1959).PubMedGoogle Scholar
  54. 3.
    Gregg, C. T., C. R. Heisler and L. F. Remmert: Biochim. biophys. Acta 45, 561 (1960).PubMedGoogle Scholar
  55. 4.
    Siekevitz, P., H. Low, L. Ernster and O. Lindberg: Biochim. biophys. Acta 29, 378 (1958).PubMedGoogle Scholar
  56. 5.
    Devlin, T. M., and A. L. Leiininger: J. biol. Ch. 233, 1586 (1958).Google Scholar
  57. 6.
    Cooper, G, and A. L. Leiininger: J. biol. Ch. 219, 489 (1956).Google Scholar
  58. 7.
    Hogeboom, G. IL, W. G Schneider and G. E. Pallade: J. biol. Ch. 172, 619 (1948).Google Scholar
  59. 1.
    Kielley, W. W., and J. R. Bronk: Biochim. biophys. Acta 23, 448 (1957).PubMedGoogle Scholar
  60. 2.
    Bronk, J. K., and W. W. Kielley: Biochim. biophys. Acta 24, 440 (1957).PubMedGoogle Scholar
  61. 3.
    Kielley, W. W., and R. K. Kielley: J. biol. Ch. 191, 485 (1951).Google Scholar
  62. 4.
    Gamble Jr., J. L., and A. L. Lehninger: J. biol. Ch. 223, 921 (1956).Google Scholar
  63. 5.
    Schneider, W. C: J. biol. Ch. 176, 259 (1948).Google Scholar
  64. 6.
    Cooper, C: J. biol. Ch. 235, 1815 (1960).Google Scholar
  65. 7.
    Kielley, W. W., and R. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  66. 1.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  67. 2.
    Penniall, R.: Biochim. biophys. Acta 44, 395 (1960).PubMedGoogle Scholar
  68. 3.
    Tabata, T., D. Szafarz and L. Wyssmann: Bull. Soc. Chim. biol. 41, 1605 (1959).PubMedGoogle Scholar
  69. 4.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Racker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  70. 5.
    Braganca, B. M., and I. Aravindakshan: Biochem. biophys. Res. Comm. 3, 484 (1960).Google Scholar
  71. 6.
    Petrushka, E., J. H. Quastel and P. G. Scholefield: Canad. J. Biochem. Physiol. 37, 989 (1959).PubMedGoogle Scholar
  72. 7.
    Beyer, R. E.: Biochim. biophys. Acta 41, 552 (1960).PubMedGoogle Scholar
  73. 8.
    Penefsky, II. S., M. E. Pullman, A. Datta and E. Racker: J. biol. Ch. 235, 3330 (1960).Google Scholar
  74. 9.
    Green, D. E., R. L. Lester and D. M. Ziegler: Biochim. biophys. Acta 23, 516 (1957).PubMedGoogle Scholar
  75. 10.
    Crane, F. L., J. L. Glenn and D. E. Green: Biochim. biophys. Acta 22, 475 (1956).PubMedGoogle Scholar
  76. 11.
    Nossal, P. M.: Austral. J. exp. Biol. med. Sci. 31, 583 (1953).Google Scholar
  77. 1.
    Penefsky, H. S., M. E. Pullman, A. Datta and E. Racker: J. biol. Ch. 235, 3330 (1960).Google Scholar
  78. 2.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  79. 3.
    Hunter jr., F. M.: 3. Int. Congr. Biochem. Bruxelles 1956, p. 298.Google Scholar
  80. 4.
    Lehninger, A. L.: Fed. Proc. 19, 952 (1960).PubMedGoogle Scholar
  81. 5.
    Drysdale, G. R., and M. Cohn: J. biol. Ch. 233, 1574 (1958).Google Scholar
  82. 1.
    Wadkins, C. L.: J. biol. Ch. 235, 3300 (1960).Google Scholar
  83. 2.
    Sacktor, B.: J. gen. Physiol. 36, 371 (1953).PubMedCentralPubMedGoogle Scholar
  84. 3.
    Cooper, C, and A. L. Lehninger: J. biol. Ch. 224, 547 (1957).Google Scholar
  85. 4.
    Kielley, W. W., and K. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  86. 5.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Backer: J. biol. Ch. 235, 3322 (1960).Google Scholar
  87. 6.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  88. 7.
    Chappell, J. B., and G. D. Greville: Nature 174, 930 (1954).PubMedGoogle Scholar
  89. 8.
    Weinbach, E. C: J. biol. Ch. 221, 609 (1956).Google Scholar
  90. 9.
    Wadkins, C. L.: J. biol. Ch. 236, 221 (1961).Google Scholar
  91. 10.
    Lardy, H. A., D. Johnson and W. C. McMurray: Arch. Biochem. 78, 587 (1958).PubMedGoogle Scholar
  92. 11.
    Witter, R. F., and W. Mink: J. biophys. biochem. Cytol. 4, 73 (1958).PubMedCentralPubMedGoogle Scholar
  93. 12.
    Penniall, R.: Biochim. biophys. Acta 30, 247 (1958).PubMedGoogle Scholar
  94. 13.
    Klemperer, H. Gl.: Biochim. biophys. Acta 23, 404 (1957).PubMedGoogle Scholar
  95. 14.
    Blecher, M., and A. White: J. biol. Ch. 235, 3404 (1960).Google Scholar
  96. 15.
    Cooper, C: J. biol. Ch. 235, 1815 (1960).Google Scholar
  97. 1.
    Cooper, C: J. biol. Ch. 235, 1815 (1960).Google Scholar
  98. 2.
    Wadkins, C. L.: J. biol. Ch. 235, 3300 (1960).Google Scholar
  99. 3.
    Slocum, D. H., and J. E. Varner: J. biol. Ch. 235, 492 (1960).Google Scholar
  100. 4.
    Pressman, B. C, and H. A. Lardy: Biochim. biophys. Acta 21, 458 (1956).PubMedGoogle Scholar
  101. 5.
    Polis, B. D., and H. W. Shmukler: J. biol. Ch. 227, 419 (1957).Google Scholar
  102. 6.
    Hülsmann, W. C, W. B. Elliot and E. C. Slater: Biochim. biophys. Acta 39, 267 (1960).PubMedGoogle Scholar
  103. 7.
    Lehninger, A. L., and L. F. Remmert: J. biol. Ch. 234, 2459 (1959).Google Scholar
  104. 8.
    Lewis, S. E., and K. S. Fowler: Biochim. biophys. Acta 38, 564 (1960).PubMedGoogle Scholar
  105. 9.
    Wojtczak, L., and A. B. Wojtczak: Biochim. biophys. Acta 39, 277 (1960).PubMedGoogle Scholar
  106. 10.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  107. 11.
    Potter, V. E., P. Siekevitz and H. C. Simonson: J. biol. Ch. 205, 893 (1953).Google Scholar
  108. 12.
    Cooper, C, and A.L. Lehninger: J. biol. Ch. 224, 547 (1957).Google Scholar
  109. 13.
    Bronk, J. R., and W. W. Kielley: Biochim. biophys. Acta 24, 440 (1957).PubMedGoogle Scholar
  110. 14.
    Myers, D. K., and E. C. Slater: Biochem. J. 67, 558 (1957).PubMedGoogle Scholar
  111. 15.
    Penniall, R.: Am. Soc. 82, 6195 (1960).Google Scholar
  112. 16.
    Beyer, R. E.: Biochim. biophys. Acta 41, 552 (1960).PubMedGoogle Scholar
  113. 17.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Racker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  114. 1.
    Löw, H.: Biochim. biophys. Acta 32, 1 (1959).PubMedGoogle Scholar
  115. 2.
    Löw, H.: Biochim. biophys. Acta 32, 11 (1959).PubMedGoogle Scholar
  116. 3.
    Beyer, E. E.: Exp. Cell Ees. 21, 217 (1960).Google Scholar
  117. 4.
    Penniall, E.: Am. Soc. 82, 6195 (1960).Google Scholar
  118. 5.
    Beyer, E. E., S. L. Lamberg and M. A. Neyman: Canad. J. Biochem. Physiol. 39, 73 (1961).Google Scholar
  119. 6.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Eacker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  120. 7.
    Kielley, W. W., and E. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  121. 8.
    Bowen, W. J., and T. D. Kerwin: J. biol. Ch. 211, 237 (1954).Google Scholar
  122. 9.
    Siekevitz, P., H. Low, L. Ernster and O. Lindberg: Biochim. biophys. Acta 29, 378 (1958).PubMedGoogle Scholar
  123. 10.
    Chappell, J. B., and S. V. Perry: Biochem. J. 55, 586 (1953).PubMedGoogle Scholar
  124. 11.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  125. 12.
    Myers, D. K., and E. C. Slater: Biochem. J. 67, 558 (1957).PubMedGoogle Scholar
  126. 1.
    Siekevitz, P., H. Löw, L. Ernster and O. Lindberg: Biochim. biophys. Acta 29, 378 (1958).PubMedGoogle Scholar
  127. 2.
    Wadkins, C. L.: J. biol. Ch. 235, 3300 (1960).Google Scholar
  128. 3.
    Pullman, M. E., H. S. Penefsky, A. Datta and E. Eacker: J. biol. Ch. 235, 3322 (1960).Google Scholar
  129. 4.
    Lardy, H. A., D. Johnson and W. C. Me Murray: Arch. Biochem. 78, 587 (1958).Google Scholar
  130. 5.
    Lardy, H. A., and H. Wellman: J. biol. Ch. 201, 357 (1953).Google Scholar
  131. 6.
    Beyer, E. E.: Biochim. biophys. Acta 41, 552 (1960).PubMedGoogle Scholar
  132. 7.
    Low, H.: Biochim. biophys. Acta 32, 1 (1959).PubMedGoogle Scholar
  133. 8.
    Low, H.: Biochim. biophys. Acta 32, 11 (1959).PubMedGoogle Scholar
  134. 9.
    Myers, D. K., and E. C. Slater: Biochem. J. 67, 572 (1957).PubMedGoogle Scholar
  135. 10.
    Witter, E. F., and W. Mink: J. biophys. biochem. Cytol. 4, 73 (1958).PubMedCentralPubMedGoogle Scholar
  136. 1.
    Blecher, M., and A. White: J. biol. Ch. 235, 3404 (1960).Google Scholar
  137. 2.
    Kielley, W. W., and R. K. Kielley: J. biol. Ch. 200, 213 (1953).Google Scholar
  138. 3.
    Cooper, C, and A. L. Lehninger: J. biol. Ch. 224, 547 (1957).Google Scholar
  139. 4.
    Wadkins, C. L.: J. biol. Ch. 235, 3300 (1960).Google Scholar
  140. 5.
    Martin, J. B., and D. M. Doty: Analyt. Chem., Washington 21, 965 (1949).Google Scholar
  141. 6.
    Pullman, M. E., H. S. Penefsky, A. Datta, and E. Racker: J. biol. Ch. 235, 3322 (1960).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1967

Authors and Affiliations

  • Masahiro Chiga
    • 1
  1. 1.From the Department of PathologyUniversity of Utah, College of MedicineSalt Lake CityUSA

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