A Radioimmunassay Using Cellulose-Bound Antibodies and its Application to Staphylococcal-α-Toxin / Eine Radioimmunprobe mit Cellulose-gebunden en Antikörpern und ihre Anwendung auf Staphylokokken-α-Toxin
Immunglobulins can be covalently bound to cellulose substituted with bromacetyl groups. The conjugates fix labelled (jodine) and unlabelled antigens selectively. Therefore, they are useful for solid phase radio-immunassay of various peptides and proteins. Specific conjugates have been successfully applied for the determination of heterologous albumin, Trasylol® and especially staphylococcal toxin in rabbit blood. Pure staphylococcal α-toxin has been jodinated with 125J resp.131 J according to Greenwood et al. Unlabelled toxin, when injection into rabbits in sublethal amounts (about 1 μg/kg or less) disappears very quickly from the blood stream. The half-life time is less than five minutes. The same is true for jodinated toxin. The slope of the decay indicates the participation of at least two processes. Binding of the hemolysin to erythrocytes is negligible. The labelled toxin does not return into the blood stream when antitoxin is injected intravenously a few minutes afterwards. Previous injection of antiotoxic or normal bovine serum delays, however, the disappearance of labelled toxin. The mode of toxin distribution in rabbit tissues and its fixation are still unknown. In mice, labelled toxin is concentrated in the kidneys shortly after injection and then distributed over various organs, especially the stomach and the gut, whereas the brain takes up only small amounts of labelled material.