Ein Substanz P-Standardpräparat

  • J. Baldauf
  • K. Gebhardt


Substanz P-Standardpräparat Hirnpeptid 


  1. 1

    Fraction Fa of crude substance P (SP), made from brain and gut, causes, in contrast to the SP peptide Fb, a relaxation of the isolated rat duodenum. Fraction Fa is therefore not a suitable SP standard preparation.

  2. 2

    By means of sephadex gel filtration, a relaxing component can be separated from the contracting Fa peptide.

  3. 3

    The relaxing Fa component is acidic, but is neither a peptide nor prosta-glandin F

  4. 4

    Purified Fa material from brain is recommended as SP standard preparation.



Substance P-Standard Preparation Brain Peptide 


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  1. Baldauf, J., Dobek, W., Zetler, G.: Freie und gebundene Substanz P-Peptide in Cortex und Subcortex des Rinderhirns. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path, (im Druck) (1969).Google Scholar
  2. Dobek, W., Zetler, G. — Zetler, G.: Darmkontrahierende Hirnpeptide in Cortex und Subcortex. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 260, 242–253 (1968).CrossRefGoogle Scholar
  3. Bertaccini, G., Cei, K.M., Erspamer, V.: The action of Physalaemin on the systemic arterial blood pressure of some experimental animals. Brit. J. Pharmacol. 25, 380–391 (1965).PubMedGoogle Scholar
  4. Cleugh, J., Gaddum, J.H., Holton, P., Leach, E.: Assay of substance P on the fowl rectal caecum. Brit. J. Pharmacol. 17, 144–158 (1961).PubMedGoogle Scholar
  5. Erspamer, V., Anastasi, A.: Polypeptides active on plain muscle in the amphibian skin: In: Hypotensive peptides. Hrsg. von E.G. Erdös, N. Back, F. Sicuteri, S. 63. Berlin-Heidelberg-New York: Springer 1966.CrossRefGoogle Scholar
  6. Euler, U.S.V.: Herstellung und Eigenschaften von Substanz P. Acta physiol. scand 4, 373–385 (1942).CrossRefGoogle Scholar
  7. Hohnes, S.W., Horton, E.W.: The identification of four prostaglandins in dog brain and their regional distribution in the central nervous system. J. Physiol. (Lond.) 195, 731–741 (1968).Google Scholar
  8. Hori, S.: The presence of bradykinin-like polypeptide, kinin-releasing and destroying activity in brain. Jap. J. Physiol. 18, 746–771 (1968).CrossRefGoogle Scholar
  9. Lembeck, F., Fischer, G.: Gekreuzte Tachyphylaxie von Peptiden. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 258, 452–456 (1967).CrossRefGoogle Scholar
  10. Fischer, G. — Starke, K.: Substanz P und Speichelsekretion. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 259, 375–385 (1968a).CrossRefGoogle Scholar
  11. Fischer, G. — Behandlung von Substanz P-Präparaten mit Aluminiumoxid. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 259, 307–313 (1968b).CrossRefGoogle Scholar
  12. Fischer, G. — Zetler, G.: Substance P. A polypeptide of possible physiological significance, expecially within the nervous system. Int. Rev. Neurobiol. 4, 159–215 (1962).Google Scholar
  13. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurements with the Folin phenol reagent. J. biol. Chem. 193, 265 (1951).PubMedGoogle Scholar
  14. Shaw, J.E., Ramwell, P.W.: Release of a substance P polypeptide from the cerebral cortex. Amer. J. Physiol. 215, 262–267 (1968).PubMedGoogle Scholar
  15. Zetler, G.: Zwei neue pharmakologisch aktike Polypeptide in einem Substanz Phaltigen Hirnextrakt. Naunyn-Schmiedebergs Arch. exp. Path. Pharmak. 242, 330–352 (1961).CrossRefGoogle Scholar
  16. — Über das Vorkommen pharmakologisch aktiver Substanzen mit Polypeptid-Charakter in Extrakten aus Darm und Gehirn. Naunyn-Schmiedebergs Arch, exp. Path. Pharmak. 246, 504–518 (1964).CrossRefGoogle Scholar
  17. — Baldauf, J.: Chromatographische Analyse eines Substanz P-Präparates aus Gehirn. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 256, 86–98 (1967).CrossRefGoogle Scholar
  18. — Mönkemeier, D., Wiechell, H.: Peptid-Rezeptoren für Tachykinine in der Tuba uterina des Menschen. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 262, 97–111 (1969).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • J. Baldauf
    • 1
  • K. Gebhardt
    • 1
  1. 1.Institut für Pharmakologie und ToxikologieUniversität des SaarlandesHomburg/SaarDeutschland

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