Biochemical and Histochemical Studies on the Distribution of Histamine in the Digestive Tract of Man, Dog and Other Mammals

  • Wilfried Lorenz
  • A. Schauer
  • St. Heitland
  • R. Calvoer
  • E. Werle


  1. 1

    The distribution of histamine was determined in tissues of the digestive tract of man, dog, pig, cow, and sheep, especially in the oral mucosa, stomach, gallbladder, and pancreas.

  2. 2

    After treatment with compound 48/80, histamine was released from the frenulum linguae, soft palate, tongue, and thyroid gland of dogs, but not from the vestibulum oris, hard palate, pharynx, oesophagus, stomach, and pancreas (experiments in the dog). The release of histamine from the tongue showed regional differences and was lowest in the root and highest in the tip.

  3. 3

    A parallelism could be shown between the histamine content and the mast cell density in different parts of the tongue, stomach and in the soft palate of untreated dogs and dogs treated with 48/80. The mast cells in the gastric mucosa could be characterized by their staining properties as “atypical” mast cells, whereas those in the musculature of the tongue were “typical” mast cells.

  4. 4

    The histamine content of the single mast cell was similar in all tissues (3.2 pg/ cell in the tongue, 3.3 in the stomach, 4.8 in the soft palate and 3.4 in the sub-maxillary gland). Only the mast cells in the fundic mucosa showed a significantly lower histamine content (1.9 pg/cell). The mast cells of the fundus and body of the stomach of the dog seemed to store histamine which was released by 48/80.

  5. 5

    A new classification of histamine stores is proposed: “unspecific mast cell stores” and “tissue specific stores”.



Histamine Alimentary Canal Mammals Mast Cells 


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  1. Aesdel, P.P. van, Jr., and R.E. Bray: On the release of histamine from rat peritoneal mast cells by compound 48/80: Effects of metabolic inhibitors. J. Pharmacol. exp. Ther. 133, 319 (1961).Google Scholar
  2. Arvy, L., et D. Quivy: Relation entre la richesse en labrocytes et la teneur en histamine des divers segments du tube digestif chez le chien. C.R. Soc. Biol. (Paris) 149, 658 (19Google Scholar
  3. Arvy, L., et D. Quivy: — Données sur la répartition des labrocytes chez le chien. C.R. Ass. Anat. 42, 234 (1955a).Google Scholar
  4. Aubes, D., R. Håkanson, Ch. Owman, and B. Sporrong: Cellular stores of histamine and monamines in the dog stomach. Life Sci. 7, 1147 (1968).Google Scholar
  5. Boréus, L.O., and N. Chakravarty: The histamine content of guinea-pig mast cells. Experientia (Basel) 16, 192 (1960).CrossRefGoogle Scholar
  6. Brodie, B.B., M.A. Beaven, F. Erjevec, and H.L. Johnson: Uptake and release of H3-histamine. In: Mechanisms of release of biogenic amines, Wenner-Gren Center International Symposium Series, Vol. 5, p. 401. Oxford: Pergamon Press 1966.Google Scholar
  7. Code, C.F.: The source in blood of the histamine like contituent. J. Physiol. (Lond.) 90, 349 (1937).Google Scholar
  8. — Histamine and gastric secretion a later look 1955–1965. Fed. Proc. 25, 1311 (1965).Google Scholar
  9. Ehinger, B., R. Håkanson, Ch. Owman, and B. Sporrong: Histochemical demonstration of histamine in paraffin sections by a fluorescence method. Bio-chem. Pharmacol. 17, 1997 (1968).CrossRefGoogle Scholar
  10. Ellenberger, W., u. H. Baum: Handbuch der vergleichenden Anatomie der Haustiere, S. 419. Berlin: A. Hirschwald 1912.Google Scholar
  11. Enerbäck, L.: Mast cells in rat gastrointestinal mucosa. 1. Effects of fixation. Acta path, microbiol. scand. 66, 289 (1966a).Google Scholar
  12. — Mast cells in rat gastrointestinal mucosa. 2. Dye-binding and metachromatic properties. Acta path, microbiol. scand. 66, 303 (1966b).Google Scholar
  13. — Mast cells in rat gastrointestinal mucosa. 3. Reactivity towards compound 48/80. Acta path, microbiol. scand. 66, 313 (1966c).Google Scholar
  14. — Mast cells in rat gastrointestinal mucosa. 4. Monoamine storing capacity. Acta path, microbiol. scand. 67, 365 (1966 d).Google Scholar
  15. Feldberg, W., and G.W. Harris: Distribution of histamine in the mucosa of the gastro-intestinal tract of the dog. J. Physiol. (Lond.) 120, 352 (1953)Google Scholar
  16. —, and J. Talesnik: Reduction of tissue histamine by compound 48/80. J. Physiol. (Lond.) 120, 550 (1953)Google Scholar
  17. Gastpar, H., u. W. Lorenz: Speicherung, Bildung und Umsatz von Histamin in Speicheldrüsen und Tonsillen. Arch. klin. exp. Ohr.-, Nas.-u. Kehlk. Heilk. 191, 715 (1968).CrossRefGoogle Scholar
  18. Graham, H.T., D.H. Lowry, N. Wahl, and M.K. Priebat: Mast cells as sources of tissue histamine. J. exp. Med. 102, 307 (1955).PubMedCentralPubMedCrossRefGoogle Scholar
  19. Håkanson, R., B. Lilja, and Ch. Owman: Properties of a new system of amine-storing cells in the gastric mucosa of the rat. Europ. J. Pharmacol. 1, 188 (1967a).CrossRefGoogle Scholar
  20. —, and S. Thunell: Changes in gastric secretion induced by certain amines, amine precursors and related enzyme inhibitors. Europ. J. Pharmacol. 1, 425 (1967b).CrossRefGoogle Scholar
  21. — Ch. Owman, and N.O. Sjöberg: Cellular stores of gastric histamine in the developing rat. Life Sci. 6, 2535 (1967).PubMedCrossRefGoogle Scholar
  22. Juhlin, L., and W.B. Shelley: Detection of histamine by a new fluorescent ophthalaldehyde stain. J. Histochem. Cytochem. 14, 525 (1966).PubMedCrossRefGoogle Scholar
  23. Kataoka, K., and E. De Robertis: Histamine in isolated small nerve endings and synaptic vesicles of rat brain cortex. J. Pharmacol. exp. Ther. 156, 114 (1967).PubMedGoogle Scholar
  24. Lorenz, W.: Lokalisation, Stoffwechsel und Frage einer physiologischen Funktion von Histamin in Speicheldrüsen, Schilddrüse und Magen. Habilitationsschrift, München 1969.Google Scholar
  25. — M. Gerant, and E. Werle: Induction of the specific histidine decarboxylase by TSH and 2-mercaptobenzimidazole-1, 3-dimethylol (MBI). Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 260, 171 (1968a).CrossRefGoogle Scholar
  26. St. Halbach, M. Gerant, and E. Werle: Specific histidine decarboxylases in the gastric mucosa of man and other mammals: Determination, location and properties. Biochem. Pharmacol. (in press) (1969).Google Scholar
  27. — G. Haubensak, M. Hutzel u. E. Werle: Histaminliberierung in Gl. submaxillaris und Pankreas durch Parasympathicomimetica, Peptidhormone, Histamin und Mepyramin. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 260, 416 (1968b).CrossRefGoogle Scholar
  28. — St. Heitland, A. Schauer, H. Gastpar u. E. Werle: Histamin in Speicheldrüsen, Tonsillen und Thymes und adaptative Histaminbildung in der Gl. submaxillaris. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 259, 319 (1968c).CrossRefGoogle Scholar
  29. — M. Hutzel, E. Matekja, and E. Werle: In preparation.Google Scholar
  30. —, u. K. Pfleger: Stoffwechsel und physiologische Funktion von Histamin im Magen. Klin. Wschr. 46, 57 (1968d).CrossRefGoogle Scholar
  31. — u. E. Werle: In vitro-Hemmung der Histidindecarboxylasen durch antithyreoidale Substanzen. Biochem. Pharmacol. 17, 539 (1968e).Google Scholar
  32. —, and E. Werle: Inhibition of the salivary secretion by antihistaminic drugs and the question of a physiological rôle of histamine in the submaxillary and parotid glands, p. 362. Abstr. Intern. Congress of Pharmacology, Basel (1969a).Google Scholar
  33. — Occurrence, cellular distribution and subcellular localization of histamine in man, animals and plants. Int. Encyclopedia of Pharmacology and Therapeutics. Oxford: Pergamon Press 1969 (in press).Google Scholar
  34. Macintosh, F. C, and W.D.M. Paton: The liberation of histamine by certain organic bases. J. Physiol. (Lond.) 109, 190 (1949).Google Scholar
  35. Minard, D.: The presence and distribution of histamine in blood. Amer. J. Physiol. 132, 327 (1941).Google Scholar
  36. Mota, I., W.T. Beraldo, A.G. Ferri, and L.C. U. Junqueira: Intracellular distribution of histamine. Nature (Lond.) 174, 698 (1954).CrossRefGoogle Scholar
  37. — A.G. Ferri, and S. Yoneda: The distribution of mast-cells in the digestive tract of laboratory animals: its bearings on the problem of the location of histamine in tissues. Quart. J. micr. Sci. 97, 251 (1956).Google Scholar
  38. Riley, J.F.: The mast cells. Edinburgh-London: E. &. S. Livingstone 1959.Google Scholar
  39. —, and G.B. West: The presence of histamine in tissue mast cells. J. Physiol. (Lond.) 120, 528 (1953).Google Scholar
  40. Schauer, A.: The mast cell. In: Veröffentlichungen aus der morphologischen Pathologie, F. Buchnerund W. Giese(Eds.). Stuttgart: G. Fischer 1964.Google Scholar
  41. Schauer, A., u. E. Werle: Zur histochemischen Darstellung des Histamins der Mastzellen. Z. ges. exp. Med. 181, 100 (1959).CrossRefGoogle Scholar
  42. Schayer, R.W.: Formation and binding of histamine by free mast cells of rat peritoneal fluid. Amer. J. Physiol. 196, 189 (1956).Google Scholar
  43. — Evidence that induced histamine is an intrinsic regulator of the microcirculatory system. Amer. J. Physiol. 202, 66 (1962).PubMedGoogle Scholar
  44. Selye, H.: The mast cells, p. 95. London: Butterworths 1965.Google Scholar
  45. Shore, P.A., A. Burkhalter, and V.H. Cohn, Jr.: A method for the fluorometric assay of histamine in tissues. J. Pharmacol. exp. Ther. 127, 182 (1959).PubMedGoogle Scholar
  46. Snyder, S.H., J. Glowinski, and J. Axelrod: The physiological disposition of H3-histamine in the rat brain. J. Pharmacol. exp. Ther. 153, 8 (1966).Google Scholar
  47. Thunberg, R.: Localization of cells containing and forming histamine in the gastric mucosa of the rat. Exp. Cell Res. 47, 108 (1967).PubMedCrossRefGoogle Scholar
  48. Uvnäs, B., and I.L. Thon: A physico-chemical model of histamine release from mast cells. In: Mechanisms of release of biogenic amines. Proc. International Wenner Gren Center Symposium Series, Vol. 5, p. 361. Oxford: Pergamon Press 1966.Google Scholar
  49. Weinshelbaum, E.J., and D.J. Ferguson: Localization of carbon-14 from histamine in enterochromaffin cells. Gastroenterology 51, 1028 (1968).Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • Wilfried Lorenz
    • 1
  • A. Schauer
    • 1
  • St. Heitland
    • 1
  • R. Calvoer
    • 1
  • E. Werle
    • 1
  1. 1.Institut für Klinische Chemie und Klinische Biochemie and Pathologisches InstitutUniversität MünchenMünchen 15Deutschland

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