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A Muscarinic Inhibition of the Noradrenaline Release Evoked by Postganglionic Sympathetic Nerve Stimulation

  • K. Löffelholz
  • E. Muscholl
Chapter

Summary

  1. 1

    The noradrenaline output from isolated rabbit hearts perfused with Tyrode solution was estimated fluorimetrically. The postganglionic sympathetic nerves of the heart were stimulated (10 shocks/sec; 1 msec) for three 1 min periods with intervals of 10 min.

     
  2. 2

    The noradrenaline output evoked by 3 consecutive stimulation periods decreased exponentially.

     
  3. 3

    Acetylcholine (10−9—10−6 g/ml) administered continuously one min before to one min after the second stimulation caused a dose-dependent reduction of the noradrenaline output evoked by the second stimulation to as low as 19% of the normal value. Acetylcholine in the concentrations applied did not cause a noradrenaline output by itself.

     
  4. 4

    The inhibitory action of acetylcholine 10−6 g/ml was fully antagonized by atropine 10−6 g/ml, whereas hexamethonium 3×10−6 g/ml had no significant antagonistic effect.

     
  5. 5

    The noradrenaline output caused by nerve stimulation was not decreased in the presence of DMPP 10−6 g/ml. DMPP 10−5 g/ml applied 3 min before electrical nerve stimulation caused an output of noradrenaline for 2 min but did not inhibit the noradrenaline release by nerve stimulation.

     
  6. 6

    Tyramine 5 × 10−6 g/ml was administered to the rabbit heart for two 6 min periods at an interval of 15 min. Methacholine 7.4 × 10−5 g/ml or atropine 10−6 g/ml if present during the second tyramine infusion did not alter the noradrenaline output produced by tyramine.

     
  7. 7

    It is concluded that low concentrations of acetylcholine by stimulating muscarinic inhibitory receptors interfere with the noradrenaline release from the postganglionic sympathetic nerve fibres evoked by electrical nerve stimulation. The possibility of a peripheral direct interaction of the cholinergic with the adre-nergic nervous system is discussed.

     

Keywords

Acetylcholine Sympathetic Nerve Ending Release of Noradrenaline DMPP Tyramine 

Schlüsselwörter

Acetylcholin Sympathische Nervenendigung Noradrenalinfreisetzung DMPP Tyramin 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • K. Löffelholz
    • 1
  • E. Muscholl
    • 1
  1. 1.Pharmakologisches Institut der UniversitätMainzDeutschland

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