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Zur Frage der Bedeutung des Kininsystems beim thermischen Ödem der Rattenpfote

  • D. Urbanitz
  • H. Wiegand
  • E. Habermann
Chapter

Schlüsselwörter

Kinine Permeabilität Hitze Entzündung 

Summary

  1. 1.

    The subcutis of rat paws heated (46, 5° C) in situ has been perfused. Kinin activity could be demonstrated regularly in the fluid which was collected in ice. When the solutions were tested immediately after having passed the tissue, only some of the experiments yielded positive results. Native and 125J-labelled kininogen as well as kininogenase and kininase activities passed into the perfusates. The sensitivity to dextran and the kinin release on heating were, in contrast to recent reports, not correlated.

     
  2. 2.

    The release of the components of the kinin system approximately paralleled that of labelled human albumin. Their concentration rose until about 1 hour after the start of the heating. There was no priority of the components of the kinin system when compared with human albumin which can be regarded as permeability indicator.

     
  3. 3.

    Intravenously injected carboxypeptidase B, because of its lower molecular weight, entered the interstitial fluid more easily than did the plasma carboxypeptidase N. Its blood level decreased rapidly; but sufficient tissue concentrations could be maintained by intravenous infusions. Neither the volume nor the time dependence of the thermic edema changed during carboxypeptidase B-infusions. The same was true for infusions of trasylol, whereas phenylbutazone inhibited the edema significantly. Edema formed by short heating (30 sec, 55° C) was equally resistant to carboxypeptidase B.

     
  4. 4.

    In the skin and muscles of the heated rat paw, carbon particles mainly stained the capillary walls. This finding argues against a considerable involvement of “classical” mediators which should induce venular lesions.

     
  5. 5.

    Infusion of large amounts of bradykinin into the arterial supply did not imitate the thermic edema; neither has bradykinin been found in the perfusate of the subcutis.

     
  6. 6.

    In the light of these findings, a significant role of the kinin system in the thermic edema of the rat paw is to be doubted.

     

Key-Words

Kinins Permeability Heat Inflammation 

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Literatur

  1. Cotran, R.S.: The delayed and prolonged vascular leakage in inflammation. II. An electron microscopic study on the vascular response after thermal injury. Amer. J. Path. 46, 583–620 (1965).Google Scholar
  2. —, and G. Majno: The delayed and prolonged vascular leakage in inflammation. I. Topography of the leaking vessels after thermal injury. Amer. J. Path. 45, 261–281 (1964).PubMedGoogle Scholar
  3. Diniz, C.R., J.F. Carvalho, J. Ryan, and M. Rochae Silva: A micromethod for the determination of bradykininogen in plasma. Nature (Lond.) 192, 1194–1195 (1961).CrossRefGoogle Scholar
  4. Edery, H., and G.P. Lewis: Plasma kinin forming enzyme activity in lymph after tissue injury. J. Physiol. (Lond.) 163, 48–49 (1962).Google Scholar
  5. Eisen, V.: Observations on intrinsic kinin forming factors in human plasma: the effect of acid, acetone, chloroform, heat, and euglobulin separation on kinin formation. J. Physiol. (Lond.) 166, 496–513 (1963).Google Scholar
  6. Erdös, E.G., R.J. Wohler, and M.I. Levine: Blocking of the in vivo effects of bradykinin and kallidin with carboxypeptidase B. J. Pharmacol. exp. Ther. 142, 327–334 (1963).Google Scholar
  7. Folk, J.E., K.A. Piez, W.R. Carroll, and J.A. Gladner: Carboxypeptidase B: IV. Purification and characterization of the porcine enzyme. J. biol. Chem. 235, 2272–2277 (1960).PubMedGoogle Scholar
  8. Greenwood, F. C, W.M. Hunter, and J.S. Glover: The preparation of 131J-labelled human growth hormone of high specific radioactivity. Biochem. J. 89, 114–123 (1963).PubMedGoogle Scholar
  9. Habermann, E.: Über pH-bedingte Modifikationen und das Molekulargewicht von Kininogen I. Biochem. Z. 337, 440–448 (1963).PubMedGoogle Scholar
  10. Jacobsen S. and B.A. Waaler The effect of scalding on the content of kinigen and kininase in limb lymph. Brit. J. Pharmacol. 27 222–229 1966PubMedGoogle Scholar
  11. Kaller, H., F. Hoffmeister u. G. Kroneberg: Die Wirkung von Trasylol® auf verschiedene Ödemformen der Rattenpfote. Arch. int. Pharmacodyn. 161, 398–409 (1966).PubMedGoogle Scholar
  12. Lowry, O.H., N.J. Rosebrough, A.L. Farr, and R.J. Randall: Protein measurements with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)PubMedGoogle Scholar
  13. Majno, G., and G.E. Palade: Studies on inflammation. I. The effect of histamin and serotonin on vascular permeability: An electron microscopic study. J. biophys. biochem. Cytol 11, 571–605 (1961).PubMedCentralPubMedCrossRefGoogle Scholar
  14. Phelps, P., D.J. Prockop, and D.J. McCaety: Crystal induced inflammation in canine joints. III. Evidence against bradykinin as a mediator of inflammation. J. Lab. clin. Med. 68, 433–444 (1966).PubMedGoogle Scholar
  15. Rochaesilva, M.: Definition of bradykinin and other kinins. Biochem. Pharmacol. 10, 3–24 (1962).CrossRefGoogle Scholar
  16. —, and A. Antonio: Kelease of bradykinin and the mechanism of a “thermic edema (46.5°C)” in the rats paw. Med. exp. (Basel) 3, 371–382 (1960).CrossRefGoogle Scholar
  17. —, and S.R. Rosenthal: Release of pharmacologically active substances from the rat skin in vivo following thermal injury. J. Pharmacol. exp. Ther. 132, 110–116 (1961).Google Scholar
  18. Ryan, G.B., and J.V. Hukley: The drug inhibition of increased vascular permeability. J. Path. Bact. 96, 371–379 (1968).PubMedCrossRefGoogle Scholar
  19. Spector, W.G., and D.A. Willoughby: The inflammatory response. Bact. Rev. 27, 117–154 (1963).PubMedGoogle Scholar
  20. Starr, M.S., and G.B. West: Bradykinin and oedema formation in heated paws of rats. Brit. J. Pharmacol. 31, 178–187 (1967).PubMedGoogle Scholar
  21. Urbanitz, D., H. Wiegand u. E. Habermann: Thermisches Ödem der Rattenpfote und Kininsystem. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path. 263, 280 (1969).CrossRefGoogle Scholar
  22. Webster, M.E., N.S. Skinner, and W.J. Powell: Role of the kinins in vasodilatation of the sceletal muscle of the dog. Amer. J. Physiol. 212, 553–558 (1967).PubMedGoogle Scholar
  23. Wilhelm, D.L.: The mediation of increased vascular permeability in inflammation. Pharmacol. Rev. 14, 251–280 (1962).PubMedGoogle Scholar
  24. Yang, H.Y.T., and E.G. Erdös: Second kininase in human blood. Nature (Lond.) 215, 1402–1403 (1967).CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • D. Urbanitz
    • 1
  • H. Wiegand
    • 1
  • E. Habermann
    • 1
  1. 1.Pharmakologisches InstitutJustus Liebig-UniversitätGießenDeutschland

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