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Influence of Phenobarbital Pretreatment on the in Vitro Ring A Reduction of Δ4-3-Oxo-Steroids in Rat Liver Microsomes

  • Christer von Bahr
  • Belisario P. Lisboa
  • Sten Orrenius
Chapter

Summary

The metabolism of 4-androstene-3, 17-dione has been studied in rat liver microsomes. Treatment of the animals with repeated doses of phenobarbital in vivo caused an enhanced rate of formation of 5α-androstane-3, 17-dione and of polar metabolites from this steroid. On the other hand, no significant increase was found in the 5β-reductase activity present in the soluble cytoplasm after phenobarbital administration to the rats. Carbon monoxide and oxidized cytochrome c abolished the formation of polar metabolites from 4-androstene-3, 17-dione but did not inhibit the 5α-reductase activity. The present findings indicate that the hydroxy-lation and 5α-reduction reactions, although both stimulated by phenobarbital treatment of the animals, do not involve common enzyme components.

Key-Words

Liver Induction 5α-reductase 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • Christer von Bahr
    • 1
    • 2
  • Belisario P. Lisboa
    • 1
    • 3
  • Sten Orrenius
    • 1
    • 2
  1. 1.Department of BiochemistryUniversity of Stockholm and HormonlaboratorietStockholmSweden
  2. 2.Biokeminska InstitutionenKungl. Universitetet i StockholmStockholmSweden
  3. 3.HormonlaboratorietStockholm 60Sweden

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