Freie und gebundene Substanz P-Peptide in Cortex und Subcortex des Rinderhirns
SchlüsselwörterSubstanz P Aktive Peptide Gewebsbindung Rinderhirn Ultrafiltrate
Substance P (SP) was extracted from cortical and subcortical tissue of bovine brains, first by hyposmotic shock and ultrafiltration, and than from the residues by boiling at pH 1–2. The ultrafiltrates contained about 20% of total brain SP.
When chromatographed on alumina columns, the ultrafiltrates of cortex and subcortex yielded mainly the SP peptide Fa, the residue from cortex the peptide Fc, and that of subcortex the peptide Fb.
Fingerprints of the ultrafiltrates of cortex and sub cortex revealed no difference in number and position of peptide spots.
Fa, i.e. the SP principle in the strict sense, exists in brain tissue predominantly in the free form, whereas the complex molecule Fb is mainly bound to tissue particles. The cortex-specific peptide Fc is also particle-bound. The existence of Fc only in cortical tissue remains so far the only difference between the grey matter of cortex and that of subcortex.
Key-WordsSubstance P Brain Peptides Tissue Binding Bovine Brain Ultrafiltrates
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