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Occurrence and Properties of Diamine Oxidases in Salivary Glands and Gastric Mucosa of Man and other Mammals / Vorkommen und Eigenschaften von Diaminoxydasen in Speicheldrüsen und Magen des Menschen und verschiedener Säugetiere

  • J. Kusche
  • W. Lorenz
  • H. Hahn
  • E. Werle
Chapter

Abstract

In the salivary glands of different species diamine oxidases (DAO) (diamine: O2-oxidoreductases EC 1.4.3.6) could be demonstrated by the method of Lorenz et al. [2, 3] and Holmstedt and Tham [1]. The activities of these enzymes with 2.5 × 10−3 M cadaverine as substrate in the submaxillary glands of man, dog, pig and cow were 0.11 ± 0.08; 0.13 ± 0.09; 0.07 ± 0.03 and 0.11 ± 0.05 mU/mg biuret protein of the homogenate, in the parotid glands of dog, pig and cow 0.06 ± 0.05; 0.17 ± 0.07 and 0.26 ± 0.10. In the gastric mucosa DAO could be found only in cow, rat and dog, but not in man, pig, cat and guinea pig. The activities using 1 × 10−3 M histamine as substrate were very low (in cow, rat and dog 0.026, 0.01 and 0.006 mU/mg protein).

References

  1. 1.
    Holmstedt, B., and E. Tham: Acta physiol. scand. 45, 152 (1959).PubMedCrossRefGoogle Scholar
  2. 2.
    Lorenz, W., J. Kusche, H. Hahn, and E. Werle: Z. analyt. Chem. 243, 259 (1968).CrossRefGoogle Scholar
  3. 3.
    — and E. Werle: Hoppe-Seylers Z. physiol. Chem. 348, 561 (1967).PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • J. Kusche
    • 1
  • W. Lorenz
  • H. Hahn
  • E. Werle
  1. 1.Institut für Klinische Chemie und Klinische Biochemie der UniversitätMünchen 15Deutschland

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