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Blocking of Pentose Phosphate Pathway in the Brain of Rats by 6-Aminonicotinamide

  • H. Herken
  • K. Lange
Chapter

Abstract

The biosynthesis of derivatives of NAD and NADP containing 6-aminonicotinamide (6-AN) entails functional disorders in different Organs, amongst which central nervous disorders are most conspicuous (Johnson and McColl,1956; Wolf Cowen, nd Geller,1959). A continuous drop in body temperature and a prolongation of sleepjng time after application of hexobarbital which is not due to a delayed turnover of the drug, are found besides progressive paralysis (Coper and Herken,1963: Coper Hadass, and Lison, 1966; Herken, 1968a). Spectrofluorometric studies showed that the enzymic conversion of nicotinamide to 6-aminonicotinamide was particularly high in the NADP-fraction (Herken and Neuhoff, 1964; Herken, 1968b). This is important because, in the brain, KADP is present in much lower concentrations than NAD, and because the conversion of NADP to 6-AXADP will considerably change the function of the enzymes needing NADP. It was also possible to establish on isolated microsomes that the synthesis of abnormally structured nucleotides by the glycohydrolase after addition of antimetabolites of nicotinamide is faster in the presence of NADP than it is if NAD is used (Brunneman Coper, and Herken, 1963). These abnormally structured nucleotides are unable to act as hydrogen carriers in oxidoreductase systems (Dietrich Friedland, and Kaplan, 1958).

Key-Words

Pentose Phosphate Pathway 6-Aminonicotinamide 6-Phos-pho D-Gluconate: NADP Oxidoreductase 6-Phosphogluconate. 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • H. Herken
    • 1
  • K. Lange
    • 1
  1. 1.Pharmakologisches InstitutFreien UniversitätBerlin 33Deutschland

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