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Kationenabhängige Membran-ATPasen in lysosomalen Granula aus polymorphkernigen Rinderleukocyten

  • D. Hegner
Chapter

Zusammenfassung

Es wird die Isolierung von Membranen aus einer lysosomalen Granulafraktion von Rinderleukocyten beschrieben. Die Membranen enthalten eine Mg2+-(E.C. 3.6.1.4) und eine Ca2+-stimulierbare ATPase. Es wurde geprüft, ob einige Pharmaka und Gifte, welche die Permeabilität der Granulamembran für Hydrolasen beeinflussen, eine Hemmwirkung auf die ATPasen haben. Die Ergebnisse werden in bezug auf Zusammenhänge zwischen der ATPase-Funktion und der Membranpermeabilität diskutiert.

Schlüsselwörter

Leukocyten Lysosomen Membranen ATPasen Membranpermeabilität. 

Summary

The isolation of membranes from lysosomal granules of bovine polymorphonuclear leucocytes is described. The membranes contain a Mg2+ (E.C. 3.6.1.4) and Ca2+-activated ATPase. In order to determine the importance of some drugs and poisons which influence the permeability of the lysosomal membranes for hydrolases, their effect on inhibition of ATPases were tested. The results are discussed in regard to the relation between ATPase function and membrane permeability.

Key-Words

Leucocytes Lysosomes Membranes ATPase-Membrane Permeability. 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • D. Hegner
    • 1
  1. 1.Institut für Pharmakologie und Toxikologie an der Veterinärmed. FakultätJustus Liebig-UniversitätGießenDeutschland

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