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Isolierung histaminliberierender und permeationsfördernder Polypeptide aus lysosomalen Granula polymorphkerniger Leukocyten des zirkulierenden Rinderblutes

  • M. Frimmer
  • F. Lutz
Chapter

Zusammenfassung

Aus Granulocyten des peripheren Rinderblutes wurde eine lysosomenreiche Fraktion dargestellt, aus der durch Extraktion, Pikratfällung und Gelelektrophorese sieben teils saure, teils basische Polypeptide gewonnen wurden.

Zwei Fraktionen mit hohem Arginingehalt sind hochwirksame Histaminliberatoren. Eine andere basische Fraktion ist an isolierten Mastzellen weniger wirksam, erhöht aber die Gefäßdurchlässigkeit in der Kaninchenhaut weit stärker als die vorgenannten Peptide. Unsere Ergebnisse stehen in Einklang mit Befunden anderer Autoren, die an Exsudatleukocyten kleiner Laboratoriumstiere gewonnen wurden. Der wichtigste Schluß aus der vorliegenden Studie ist, daß auch der Granulocyt des zirkulierenden Blutes bereits die bei der Emigration bedeutsamen Polypeptide enthält.

Schlüsselwörter

Basische Polypeptide Histaminliberatoren Granulocyten Gefäßpermeabilität Elektrophorese. 

Summary

Isolated lysosomal granules were prepared using the method of Hegner and lysed by Triton X 100. A crude peptide was isolated from concentrated extracts by precipitation with picric acid. Seven homogeneous peptides were separated by preparative Polyvinylchloride block electrophoresis. The IEPs of these products were respectively about 2; 4.5; 6.5; 9.8; 11.2 and > 12. The amino acid composition of two basic peptides (IEPs > 12) was similar to arginine rich products found earlier in exudate leucocytes by Spitznagel and Zeya. MWs of 10,500 and 5,200 were estimated by gel filtration. These two arginine rich polypeptides were potent histamine liberators (0.5–1.0 μg/ml released more than 50% histamine from isolated peritoneal mast cells of the rat).

Another basic polypeptide (MW = 16,000-19,000, IEP = 11.2) increased the permeability of venules in rabbit skin after i.c. injection of 0.2 μg. This peptide was found to be less potent in releasing histamine. Our observations were in agreement with those of Seegers and Janoff Keller et al. In addition, not only lysosomes from leucocytes of exudates contain biologically active peptides, but also granules from PMN leucocytes of the circulating blood which store remarkable amounts of active basic products.

Key-Words

Basic Polypeptides Histamine Liberator Bovine PMN Leucocytes Vascular Permeability Electrophoresis. 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1969

Authors and Affiliations

  • M. Frimmer
    • 1
  • F. Lutz
    • 1
  1. 1.Institut für Pharmakologie und Toxikologie an der Veterinärmed. FakultätJustus Liebig-UniversitätGießenDeutschland

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