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Computergestützte Auswertung eines In-vitro-Transformationstests

  • C. Nowak
  • G. Lichtenberg
  • G. Heller
  • C. Meckert
  • H.-B. Richter-Reichhelm
Chapter

Zusammenfassung

Transformationstests als In-vitro-Nachweis von Kanzerogenen werden seit einiger Zeit zur Anwendungsreife entwickelt. Bei der Auswertung solcher Tests werden transformierte Zellkolonien mit der Stereolupe ausgezählt. Wir haben in einem Transformationstest, mit epithelialen Nierenzellen vom Hund als Zielzellen, die Möglichkeit einer automatisierten Auswertung von transformierten Kolonien im Soft Agar untersucht. Dazu wurde die Anzahl und Größe der im Soft Agar gewachsenen Kolonien durch Einsatz eines computergestützten Bildverarbeitungsprogrammes bestimmt. Die Ergebnisse nach der Kalibrierung des Systems zeigen, daß mit der hier verwendeten Methodik schnell und reproduzierbar die Anzahl der Kolonien und auch die Koloniefläche bestimmt werden können. Dadurch ist ein hohes Maß an Genauigkeit und eine Minimierung des subjektiven Auswertungsfehlers erreichbar. Diese computergestütze Auswertmethodik, die prinzipiell auch für andere Anwendungszwecke in Frage kommt, macht einen routinemäßigen Einsatz von Transformationstests erheblich kostengünstiger.

Computerized image analysis of an in vitro transformation assay

Summary

The methodology of transformation assays in in vitro carcinogenesis has been continuously improved to make them routinely applicable. In most of the recently employed transformation assays colony counting was performed under a stereo microscope. We have investigated the application of a computerized image analysing system to determine transformed colonies after in vitro cultivation of epithelial canine kidney cells. Special intention was imposed on the calibration of the system. First results show that determination of number and surface of transformed colonies in soft agar were rapidly available. In addition a high level of reproducibility and a thereby enhanced reliability could be achieved. The implementation of such a computerized image analysis in routine performance of transformation assays would enhance its robustness and applicability and minimize the bias due to evaluation under the stereo microscope to an important extent. It may also be used for similar purpos (e.g. cytogenetic tests).

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • C. Nowak
    • 1
  • G. Lichtenberg
    • 1
  • G. Heller
    • 1
  • C. Meckert
    • 1
  • H.-B. Richter-Reichhelm
    • 1
  1. 1.Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin (BgVV)BerlinDeutschland

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