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Labordiagnostik der HIV-Infektion

  • T. Porstmann
Chapter

Zusammenfassung

Die Diagnose einer HIV-Infektion wird unabhängig vom klinischen Verdacht ausschließlich durch labordiagnostische Untersuchungen gestellt und beruht auf der Tatsache, daß HIV-Infizierte Antikörper gegen die viralen Strukturproteine und viralen Enzyme bilden. Serologische Parameter dienen dabei:
  1. 1.

    dem Nachweis einer HIV-Infektion perse

     
  2. 2.

    der Prognostizierung des Krankheitsverlaufs nach erwiesener HIV-Infektion.

     

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Literatur

  1. Agbalika, F., Ferchal, F., Garnier, J.-P., Eugene, M., Bedrossian, J. and Lagrange, H., False-positive HIV-antigens related to emergence of a 25–30 kD protein detected in organ recipients. AIDS 6 (1992) 959–962PubMedCrossRefGoogle Scholar
  2. Archibald, D.W., Johnson, L.P., Nair, P., Alger, L.S., Hebert, C.A., Davis, E. and Mines, S. E., Detection of salivary immunoglobulin A antibodies to HIV-1 in infants and children AIDS 4 (1990) 417–420PubMedCrossRefGoogle Scholar
  3. Berry, N.J., Salker, R., Contreras, M., Barbara, J.A.J. and Tedder, R.S. A comparison of four enzyme immunoassays for the simultaneous detection of HIV-1 — and HIV-2-specific antibody J. Virol. Methods 34 (1991) 91–100PubMedCrossRefGoogle Scholar
  4. Fenouillet, E., Blanes, N., Coutellier, A., Demarquest, J., Rozenbaum, W. and Gluckman, J. C. Monitoring of antibodies against human immunodeficiency virus type 1 p25 core protein as prognostic marker. JID 166 (1992) 611–615PubMedCrossRefGoogle Scholar
  5. Gnann, J.W., Nelson, J.A. and Oldstone, M.B.A. Fine mapping of an immunodominant domain in the transmembrane glycoprotein of human immunodeficiency virus. J. Virol. 61 (1987)2639–2641PubMedCentralPubMedGoogle Scholar
  6. Gürtler, L.G. Diagnostik der HIV-Infektion Therapiewoche 8 (1993) 388–397Google Scholar
  7. Habermehl, K.-O. und Maass, G. Interpretation der Immunoblots zum Nachweis von Antikörpern gegen HIV-1 und HIV-2 Dt. Ärztebl. 89 (1992) 1556–1557Google Scholar
  8. Haseltine, W.A., Replication and pathology of the AIDS virus J.AIDS 1 (1988)217–240Google Scholar
  9. Healey, D. S., Maskill, W. J., Howard, T. S., Armstrong, V.A., Bolton, W. V., Cooper, G.J., Cownie, S.C., Hiley, L.A., Imrie, A.A., Johnson, S., Robertson, P. W. and Schepetiuk, S., HIV-1 Western blot: development and assessment af testing to resolve indeterminate reactivity. AIDS 6 (1992) 629–633PubMedCrossRefGoogle Scholar
  10. Kusk, P., Bugge, T.H., Lindhardt, B., Hulgaard, E.F. and Holmbäck, K. Mapping of linear B-cell epitoges on the major core protein p24 of human immunodeficiency virus type 1 (HIV-1) AIDS Res. Hum. Retrov. 8 (1992) 1789–1794CrossRefGoogle Scholar
  11. Loveday, C. and Tedder, R. D., Enzyme-linked immunosorbent assays for the measurement of human immunodeficiency virus, type 1 reverse transcriptase antigen and antibodies. J. Virol. Methods. 41 (1993) 181–192PubMedCrossRefGoogle Scholar
  12. McAlpine, L., Parry, J. V. and Tosswill, J. H.C., Evaluation of an enzyme immunoassay for the combined detection of antibodies to HIV-1, HIV-2, HTLV-I and HTLV-II AIDS 6 (1992) 387–389PubMedCrossRefGoogle Scholar
  13. Papadopulos-Eleopulos, E., Turner, V. F. and Papadimitriou, J. M., Is a positive Western blot proof of HIV infektion? Biotechn. 11 (1993) 696–707CrossRefGoogle Scholar
  14. Porstmann, T., Nugel, E., Henklein, P., Döpel, H., Rönspeck, W., Pas, P. and von Baehr, R. Two-colour combination enzyme-linked immunosorbent assay for the simultaneous detection of HBV and HIV infection J. Immunol. Methods. 158 (1993) 95–106PubMedCrossRefGoogle Scholar
  15. Spielberg, F., Ryder, R.W., Harris, J., Heyward, W.L., Kabeya, C.M., Kifuani, N.K., Beuder, Th.R. and Quinn, Th.C., Field testing and comparative evaluation of rapid, visually read screening assays for antibody to human immundeficiency virus The Lancet, i (1990) 580–584Google Scholar
  16. Stickler, M. C., Sharpe, S. and Zolla-Pamer, S. p24 antibody production in p24 antibody-negative HIV-infected subjects Viral Immunology 5, (1992) 123–132PubMedCrossRefGoogle Scholar
  17. Van Kerckhoven, G., Vercauteren, P.P. und v. der Groen, G. Comparative evaluation of 36 commercial assays for detecting antibodies to HIV WHO-Bulletin OMS 69 (1991) 753–760Google Scholar

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© Springer-Verlag Berlin Heidelberg 1993

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  • T. Porstmann

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