Extremely high electron accelerating voltages have been tried in the past in attempts to see internal detail in biological specimens up to 1 μ thick. In addition there is a slight theoretical gain in resolution as the voltage is raised. These two reasons have led to instruments for 220 kV, (1), 300 kV, (2), 400 kV, (3) and 1400 kV (4) among others. The advent of the ultra-microtome in various forms, capable of cutting sections 0.01 μ thick, has eliminated the need for penetration. The improvement in resolution has never been demonstrated since the contrast falls more rapidly as the voltage is raised. At present there is a need to follow the converse of past work and investigate the region below 10 kV for electron microscopy. The resolving power of the instrument will deteriorate only slightly while the gain in contrast may actually improve the overall resolution for a given thin section.
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