Verhandlungen pp 1473-1475 | Cite as

A negative staining technique for high resolution of viruses

  • R. W. Horne
  • S. Brenner


The aim of this study is to discuss the possibility of using negative staining techniques on unsectioned material. During investigations concerned with the detailed structure of the protein components of bacteriophage, serious limitations were encountered using existing shadow-casting and sectioning techniques.
Fig. 1

Intact T2 phage particles in PTA droplet. Striations along the tail are clearly visible. No staining of the protein is evident


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    Huxley, H. E.: Electron Microscopy Proc. Stockholm Conference, September 1956, p. 260.Google Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1960

Authors and Affiliations

  • R. W. Horne
    • 1
  • S. Brenner
    • 1
  1. 1.Cavendish LaboratoryUniversity of CambridgeEngland

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