Spectrophotometric and electronmicroscopic experiments with organic built-up films exposed to osmium tetroxide
Electron micrographs of cellular structures, fixed and stained with OsO4 or other agents, do not give any chemical information about the details of the image. Results from experiments in vitro on the reaction between lipides or proteins and OsO4 may be misleading. We have, therefore, begun a study of the interaction between OsO4 and artificially deposited multimolecular layers of known chemical substances. The approach is two-fold. Since OsO4 has a well defined absorption band with a peak at 2460 Ă it should be possible to estimate the amount of OsO4 taken up by the multilayers. Furthermore, multilayers deposited on a suitable base may be prepared for examination in the electron microscope.
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